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AB316737

Recombinant Human Sorbitol Dehydrogenase Protein Standard (His tag)

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Recombinant Human Sorbitol Dehydrogenase Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, sELISA.

View Alternative Names

Sorbitol dehydrogenase, SDH, L-iditol 2-dehydrogenase, Polyol dehydrogenase, Ribitol dehydrogenase, Xylitol dehydrogenase, RDH, XDH, SORD

2 Images
Sandwich ELISA - Recombinant Human Sorbitol Dehydrogenase Protein Standard (His tag) (AB316737)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Sorbitol Dehydrogenase Protein Standard (His tag) (AB316737)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human Sorbitol Dehydrogenase Protein Standard (His tag) (AB316737)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Sorbitol Dehydrogenase Protein Standard (His tag) (AB316737)

SDS-PAGE analysis of ab316737 under reducing conditions for 2ug protein.

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

Q00796

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

Constituents: 12.012% Urea, 8.71% L-Arginine, 5% Glycerol (glycerin, glycerine), 1% Glycine, 0.8544% disodium;hydrogen phosphate;dodecahydrate, 0.0154% Potassium phosphate monobasic

storage-buffer

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Complementary Products

Primary Antibodies

AB244731

Anti-Sorbitol Dehydrogenase antibody [EPR20780-22] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Sorbitol Dehydrogenase antibody [EPR20780-22] - BSA and Azide free (Capture) (AB244731)

  • 0
  • 0
Assay Kits

AB233613

Human Sorbitol Dehydrogenase ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (AB233613)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

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Sequence info

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"39.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q00796","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sorbitol Dehydrogenase (SDH) also known as SORD is an enzyme that catalyzes the oxidation of sorbitol to fructose. This reaction involves the conversion of a secondary alcohol group to a ketone. SDH has a molecular mass of approximately 38 kDa. It is expressed in various human tissues most importantly in the liver kidneys and seminal vesicles. The enzyme requires NAD+ as a cofactor for its catalytic activity.
Biological function summary

SDH plays a part in the polyol pathway which is important for the metabolism of glucose into fructose. It functions alongside aldose reductase to regulate the conversion of glucose into sorbitol and then sorbitol into fructose. SDH acts independently without forming a complex with other proteins. The amount and activity of SDH can affect cellular osmotic balance and energy storage.

Pathways

SDH is involved in the polyol pathway and influences sorbitol levels. An excess of sorbitol can contribute to cellular damage especially in the context of diabetes. SDH’s role is linked to the exclusive conversion of sorbitol to fructose contrasting with aldose reductase which initiates sorbitol production from glucose. Both enzymes are key in maintaining the balance between extracellular and intracellular glucose.

Alterations in SDH activity relate to diabetic complications like diabetic retinopathy and neuropathy. The accumulation of sorbitol due to insufficient SDH activity contributes to the development of these conditions. SDH's connection with aldose reductase is particularly notable here as both proteins impact the sorbitol levels that affect various tissues under hyperglycemic conditions.
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Specifications

Form

Liquid

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General info

Function

Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols. Is mostly active with D-sorbitol (D-glucitol), L-threitol, xylitol and ribitol as substrates, leading to the C2-oxidized products D-fructose, L-erythrulose, D-xylulose, and D-ribulose, respectively (PubMed : 3365415). Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. The polyol pathway is believed to be involved in the etiology of diabetic complications, such as diabetic neuropathy and retinopathy, induced by hyperglycemia (PubMed : 12962626, PubMed : 25105142, PubMed : 29966615). May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility (PubMed : 16278369). May have a more general function in the metabolism of secondary alcohols since it also catalyzes the stereospecific oxidation of (2R,3R)-2,3-butanediol. To a lesser extent, can also oxidize L-arabinitol, galactitol and D-mannitol and glycerol in vitro. Oxidizes neither ethanol nor other primary alcohols. Cannot use NADP(+) as the electron acceptor (PubMed : 3365415).

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family.

Subcellular localisation

Mitochondrion membrane

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Product protocols

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Target data

Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols. Is mostly active with D-sorbitol (D-glucitol), L-threitol, xylitol and ribitol as substrates, leading to the C2-oxidized products D-fructose, L-erythrulose, D-xylulose, and D-ribulose, respectively (PubMed : 3365415). Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. The polyol pathway is believed to be involved in the etiology of diabetic complications, such as diabetic neuropathy and retinopathy, induced by hyperglycemia (PubMed : 12962626, PubMed : 25105142, PubMed : 29966615). May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility (PubMed : 16278369). May have a more general function in the metabolism of secondary alcohols since it also catalyzes the stereospecific oxidation of (2R,3R)-2,3-butanediol. To a lesser extent, can also oxidize L-arabinitol, galactitol and D-mannitol and glycerol in vitro. Oxidizes neither ethanol nor other primary alcohols. Cannot use NADP(+) as the electron acceptor (PubMed : 3365415).
See full target information SORD
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