Recombinant Human SOS1 protein (His tag) is a Human Fragment protein, in the 562 to 1052 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A D P E E Q M R L P S A D V Y R F A E P D S E E N I I F E E N M Q P K A G I P I I K A G T V I K L I E R L T Y H M Y A D P N F V R T F L T T Y R S F C K P Q E L L S L I I E R F E I P E P E P T E A D R I A I E N G D Q P L S A E L K R F R K E Y I Q P V Q L R V L N V C R H W V E H H F Y D F E R D A Y L L Q R M E E F I G T V R G K A M K K W V E S I T K I I Q R K K I A R D N G P G H N I T F Q S S P P T V E W H I S R P G H I E T F D L L T L H P I E I A R Q L T L L E S D L Y R A V Q P S E L V G S V W T K E D K E I N S P N L L K M I R H T T N L T L W F E K C I V E T E N L E E R V A V V S R I I E I L Q V F Q E L N N F N G V L E V V S A M N S S P V Y R L D H T F E Q I P S R Q K K I L E E A H E L S E D H Y K K Y L A K L R S I N P P C V P F F G I Y L T N I L K T E E G N P E V L K R H G K E L I N F S K R R K V A E I T G E I Q Q Y Q N Q P Y C L R V E S D I K R F F E N L N P M G N S M E K E F T D Y L F N K S L E I E P R N P K P L P R F P K K Y S Y P L K S P G V R P S N P R P G T H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Promotes the exchange of Ras-bound GDP by GTP (PubMed:8493579). Probably by promoting Ras activation, regulates phosphorylation of MAP kinase MAPK3 in response to EGF (PubMed:17339331). Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity (By similarity).
Alternative names
Son of sevenless homolog 1, SOS-1, SOS1
Recommended products
Recombinant Human SOS1 protein (His tag) is a Human Fragment protein, in the 562 to 1052 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q07889-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Magnesium chloride
Sequence info
Amino acid sequence
- A D P E E Q M R L P S A D V Y R F A E P D S E E N I I F E E N M Q P K A G I P I I K A G T V I K L I E R L T Y H M Y A D P N F V R T F L T T Y R S F C K P Q E L L S L I I E R F E I P E P E P T E A D R I A I E N G D Q P L S A E L K R F R K E Y I Q P V Q L R V L N V C R H W V E H H F Y D F E R D A Y L L Q R M E E F I G T V R G K A M K K W V E S I T K I I Q R K K I A R D N G P G H N I T F Q S S P P T V E W H I S R P G H I E T F D L L T L H P I E I A R Q L T L L E S D L Y R A V Q P S E L V G S V W T K E D K E I N S P N L L K M I R H T T N L T L W F E K C I V E T E N L E E R V A V V S R I I E I L Q V F Q E L N N F N G V L E V V S A M N S S P V Y R L D H T F E Q I P S R Q K K I L E E A H E L S E D H Y K K Y L A K L R S I N P P C V P F F G I Y L T N I L K T E E G N P E V L K R H G K E L I N F S K R R K V A E I T G E I Q Q Y Q N Q P Y C L R V E S D I K R F F E N L N P M G N S M E K E F T D Y L F N K S L E I E P R N P K P L P R F P K K Y S Y P L K S P G V R P S N P R P G T H H H H H H
- Accession
- Q07889
- Protein length
- Fragment
- Predicted molecular weight
- 58 kDa
- Amino acids
- 562 to 1052
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Promotes the exchange of Ras-bound GDP by GTP (PubMed:8493579). Probably by promoting Ras activation, regulates phosphorylation of MAP kinase MAPK3 in response to EGF (PubMed:17339331). Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity (By similarity).
- Post-translational modifications
Phosphorylation at Ser-1134 and Ser-1161 by RPS6KA3 create YWHAB and YWHAE binding sites and which contribute to the negative regulation of EGF-induced MAPK1/3 phosphorylation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human SOS1 protein (His tag) (ab233594)
15% SDS-PAGE analysis of 3 μg ab233594.
MW: 50-70 kDa (SDS-PAGE under reducing conditions).
Downloads
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