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Recombinant Human SOS1 protein (His tag) is a Human Fragment protein, in the 562 to 1052 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

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Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

Biologically active

No

Sequence

A D P E E Q M R L P S A D V Y R F A E P D S E E N I I F E E N M Q P K A G I P I I K A G T V I K L I E R L T Y H M Y A D P N F V R T F L T T Y R S F C K P Q E L L S L I I E R F E I P E P E P T E A D R I A I E N G D Q P L S A E L K R F R K E Y I Q P V Q L R V L N V C R H W V E H H F Y D F E R D A Y L L Q R M E E F I G T V R G K A M K K W V E S I T K I I Q R K K I A R D N G P G H N I T F Q S S P P T V E W H I S R P G H I E T F D L L T L H P I E I A R Q L T L L E S D L Y R A V Q P S E L V G S V W T K E D K E I N S P N L L K M I R H T T N L T L W F E K C I V E T E N L E E R V A V V S R I I E I L Q V F Q E L N N F N G V L E V V S A M N S S P V Y R L D H T F E Q I P S R Q K K I L E E A H E L S E D H Y K K Y L A K L R S I N P P C V P F F G I Y L T N I L K T E E G N P E V L K R H G K E L I N F S K R R K V A E I T G E I Q Q Y Q N Q P Y C L R V E S D I K R F F E N L N P M G N S M E K E F T D Y L F N K S L E I E P R N P K P L P R F P K K Y S Y P L K S P G V R P S N P R P G T H H H H H H

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Shipping To:
United States

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant Human SOS1 protein (His tag) is a Human Fragment protein, in the 562 to 1052 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Applications

SDS-PAGE

Predicted molecular weight

58 kDa

Amino acids

562 to 1052

Sequence

A D P E E Q M R L P S A D V Y R F A E P D S E E N I I F E E N M Q P K A G I P I I K A G T V I K L I E R L T Y H M Y A D P N F V R T F L T T Y R S F C K P Q E L L S L I I E R F E I P E P E P T E A D R I A I E N G D Q P L S A E L K R F R K E Y I Q P V Q L R V L N V C R H W V E H H F Y D F E R D A Y L L Q R M E E F I G T V R G K A M K K W V E S I T K I I Q R K K I A R D N G P G H N I T F Q S S P P T V E W H I S R P G H I E T F D L L T L H P I E I A R Q L T L L E S D L Y R A V Q P S E L V G S V W T K E D K E I N S P N L L K M I R H T T N L T L W F E K C I V E T E N L E E R V A V V S R I I E I L Q V F Q E L N N F N G V L E V V S A M N S S P V Y R L D H T F E Q I P S R Q K K I L E E A H E L S E D H Y K K Y L A K L R S I N P P C V P F F G I Y L T N I L K T E E G N P E V L K R H G K E L I N F S K R R K V A E I T G E I Q Q Y Q N Q P Y C L R V E S D I K R F F E N L N P M G N S M E K E F T D Y L F N K S L E I E P R N P K P L P R F P K K Y S Y P L K S P G V R P S N P R P G T H H H H H H

Accession
Q07889-1
Protein length

Fragment

Animal free

No

Nature

Recombinant

Species

Human

Concentration
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Storage buffer

pH: 7.5
Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Magnesium chloride

Specifications

Form

Liquid

Additional notes

Affinity purified

General info

Function

Promotes the exchange of Ras-bound GDP by GTP (PubMed:8493579). Probably by promoting Ras activation, regulates phosphorylation of MAP kinase MAPK3 in response to EGF (PubMed:17339331). Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity (By similarity).

Post-translational modifications

Phosphorylation at Ser-1134 and Ser-1161 by RPS6KA3 create YWHAB and YWHAE binding sites and which contribute to the negative regulation of EGF-induced MAPK1/3 phosphorylation.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

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