Recombinant Human SOX2 protein is a Human Full Length protein, in the 1 to 317 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, HPLC, sELISA, WB.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, HPLC, sELISA, WB
- Biologically active
- No
Amino acid sequence
M Y N M M E T E L K P P G P Q Q T S G G G G G N S T A A A A G G N Q K N S P D R V K R P M N A F M V W S R G Q R R K M A Q E N P K M H N S E I S K R L G A E W K L L S E T E K R P F I D E A K R L R A L H M K E H P D Y K Y R P R R K T K T L M K K D K Y T L P G G L L A P G G N S M A S G V G V G A G L G A G V N Q R M D S Y A H M N G W S N G S Y S M M Q D Q L G Y P Q H P G L N A H G A A Q M Q P M H R Y D V S A L Q Y N S M T S S Q T Y M N G S P T Y S M S Y S Q Q G T P G M A L G S M G S V V K S E A S S S P P V V T S S S H S R A P C Q A G D L R D M I S M Y L P G A E V P E P A A P S R L H M S Q H Y Q S G P V P G T A I N G T L P L S H M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab79950 can be used as a WB positive control in conjunction with Anti-SOX2 antibody ab97959. |
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Target data
Function
The protein expressed by the gene SOX2 is critical for early embryogenesis and for the pluripotency of embryonic stem cells (PubMed:18035408). This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
Transcription factor SOX-2, SOX2
Recommended products
Recombinant Human SOX2 protein is a Human Full Length protein, in the 1 to 317 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, HPLC, sELISA, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, HPLC, sELISA, WB
- Biologically active
- No
- Accession
- P48431-1
- Animal free
- No
- Species
- Human
- Concentration
Sequence info
Amino acid sequence
- M Y N M M E T E L K P P G P Q Q T S G G G G G N S T A A A A G G N Q K N S P D R V K R P M N A F M V W S R G Q R R K M A Q E N P K M H N S E I S K R L G A E W K L L S E T E K R P F I D E A K R L R A L H M K E H P D Y K Y R P R R K T K T L M K K D K Y T L P G G L L A P G G N S M A S G V G V G A G L G A G V N Q R M D S Y A H M N G W S N G S Y S M M Q D Q L G Y P Q H P G L N A H G A A Q M Q P M H R Y D V S A L Q Y N S M T S S Q T Y M N G S P T Y S M S Y S Q Q G T P G M A L G S M G S V V K S E A S S S P P V V T S S S H S R A P C Q A G D L R D M I S M Y L P G A E V P E P A A P S R L H M S Q H Y Q S G P V P G T A I N G T L P L S H M
- Accession
- P48431
- Protein length
- Full Length
- Amino acids
- 1 to 317
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
ab79950 is greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per µg (1EU/µg).
General info
- Function
The protein expressed by the gene SOX2 is critical for early embryogenesis and for the pluripotency of embryonic stem cells (PubMed:18035408).
This supplementary information is collated from multiple sources and compiled automatically.- Post-translational modifications
Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Recombinant Human SOX2 protein (ab79950)
Standard Curve for SOX2 (Analyte: SOX2 protein (Human) (ab79950)); dilution range 1pg/ml to
1μg/ml using Capture Antibody Mouse monoclonal [57CT23.3.4] to SOX2 (ab75485) at
0.2μg/ml and Detector Antibody Rabbit monoclonal [EPR3131] to SOX2 (Anti-SOX2 antibody [EPR3131] ab92494) at
0.5μg/ml.
Western blot - Recombinant Human SOX2 protein (ab79950)
All lanes: Western blot - Anti-SOX2 antibody (Anti-SOX2 antibody ab97959) at 1/1000 dilution
All lanes: Western blot - Recombinant Human SOX2 protein (ab79950) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Exposure time: 30s
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