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AB79950

Recombinant Human SOX2 protein

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Recombinant Human SOX2 protein is a Human Full Length protein, in the 1 to 317 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, HPLC, sELISA, WB.

View Alternative Names

Transcription factor SOX-2, SOX2

2 Images
Sandwich ELISA - Recombinant Human SOX2 protein (AB79950)
  • sELISA

Unknown

Sandwich ELISA - Recombinant Human SOX2 protein (AB79950)

Standard Curve for SOX2 (Analyte : SOX2 protein (Human) (ab79950)); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [57CT23.3.4] to SOX2 (ab75485) at 0.2μg/ml and Detector Antibody Rabbit monoclonal [EPR3131] to SOX2 (ab92494) at 0.5μg/ml.

Western blot - Recombinant Human SOX2 protein (AB79950)
  • WB

Unknown

Western blot - Recombinant Human SOX2 protein (AB79950)

All lanes:

Western blot - Anti-SOX2 antibody (<a href='/en-us/products/primary-antibodies/sox2-antibody-ab97959'>ab97959</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant Human SOX2 protein (ab79950) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 34 kDa

true

Exposure time: 30s

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

WB, HPLC, SDS-PAGE, sELISA

applications

Biologically active

No

Accession

P48431

Animal free

No

Carrier free

No

Species

Human

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Primary Antibodies

AB97959

Anti-SOX2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB97959)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab79950 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/sox2-antibody-ab97959'>ab97959</a>.</p>" } } }
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Sequence info

[{"sequence":"MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":317,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P48431","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
False
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Specifications

Form

Lyophilized

Additional notes

ab79950 is greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per µg (1EU/µg).

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General info

Function

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed : 18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).

Post-translational modifications

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.. Methylation at Lys-42 and Lys-117 is necessary for the regulation of SOX2 proteasomal degradation.. Ubiquitinated by WWP2, leading to proteasomal degradation.

Subcellular localisation

Nucleus speckle

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Product protocols

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Target data

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed : 18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).
See full target information SOX2
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Product promise

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