Recombinant Human Spa-1 protein (GST tag N-Terminus)

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spa-1 also known as human SPA plays a role in the regulation of small GTPase activity specifically targeting Rap1. This protein weighing approximately 120 kDa resides within various tissues with notable expression in the hematopoietic system and neuronal tissues. Researchers use names like Anti-SPA and Anti-Spa when referring to assays designed to recognize this protein. SPA-1 functions as a Rap1 GTPase-activating protein (GAP) facilitating the conversion of active GTP-bound Rap1 to its inactive GDP-bound form.

Biological function summary

Spa-1 engages in the control of cell adhesion and migration through its interaction with the Ras-related protein Rap1. As part of larger signal transduction complexes Spa-1 influences the organization of actin cytoskeleton and impacts integrin-mediated cell adhesion. Through its GAP activity it maintains cellular responsiveness to external stimuli impacting processes like immune cell trafficking and neuronal growth. Studies have noted its significance in maintaining homeostasis in tissues where it is expressed.

Pathways

Spa-1 integrates into key signaling cascades impacting the Ras superfamily of GTPases. Within the Rap1 signaling pathway Spa-1 works alongside proteins like RapGEF to finely regulate signal transduction processes affecting cell adhesion and cytoskeletal dynamics. In the context of the integrin signaling pathway Spa-1 modulates the actions of B-Raf in connection with Rap1 dictating responses necessary for cellular motility and positioning.

Spa-1 displays relevance to leukemia and neurological conditions. Its role in negative regulation of Rap1 activity links it to the aberrant proliferation seen in certain leukemia types where altered Rap1 signaling contributes to uncontrolled cell growth. Furthermore disruptions in Spa-1's modulation of neuronal Rap1 activity have associations with neurodegenerative diseases where proper cell migration and adhesion are impaired. Research explores its connections to proteins like BCR-ABL in the context of leukemia highlighting Spa-1 as a therapeutic target in disease management.