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AB132258

Recombinant Human SPAM1 protein

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(1 Publication)

Recombinant Human SPAM1 protein is a Human Full Length protein, in the 1 to 509 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

HYAL3, PH20, SPAM1, Hyaluronidase PH-20, Hyal-PH20, Hyaluronoglucosaminidase PH-20, Sperm adhesion molecule 1, Sperm surface protein PH-20

1 Images
SDS-PAGE - Recombinant Human SPAM1 protein (AB132258)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SPAM1 protein (AB132258)

12.5% SDS-PAGE analysis of ab132258 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

P38567

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Hyaluronidase PH20.
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Sequence info

[{"sequence":"MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIVSILFLIISSVASL","proteinLength":"Full Length","predictedMolecularWeight":"84.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":509,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P38567","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPAM1 also known as sperm adhesion molecule 1 or PH-20 refers to a glycoprotein found on the sperm cell surface. Its molecular mass is approximately 64 kDa. This protein is primarily expressed in the testes and epididymis where it plays a role in sperm maturation. SPAM1 possesses hyaluronidase activity breaking down hyaluronic acid present in the cumulus cell matrix of the female reproductive tract during fertilization. It aids sperm in penetrating the egg's outer layers which is important for successful fertilization.
Biological function summary

SPAM1 participates in sperm penetration through the zona pellucida an essential step in fertilization. It acts independently facilitating sperm passage by degrading surrounding extracellular matrices. SPAM1 while not part of a larger protein complex interacts directly with the female reproductive tract's biochemical structures. These interactions ensure an efficient sperm-vs-egg recognition process that aids in sperm-egg fusion. Its enzymatic activity is central to this sperm-egg binding and membrane fusion.

Pathways

SPAM1 contributes significantly to the fertilization pathway specifically the Acrosome Reaction. It interacts indirectly with proteins like ZP3 a zona pellucida protein that triggers the acrosome reaction necessary for sperm to penetrate the ovum. Additionally SPAM1 plays a minor role in the hyaluronan-mediated motility pathway interacting faintly with the extracellular matrix components to enhance sperm motility and penetration.

SPAM1 has been implicated in certain infertility cases particularly those involving the failure of sperm to penetrate the egg successfully. This condition might relate to defects or alterations in SPAM1 activity or expression. Moreover some studies suggest a possible link between SPAM1 and testicular cancer where aberrant expression could influence tumorigenesis. SPAM1 anomalies may associate with other proteins like EZH2 involved in various cancers suggesting broader functional implications beyond reproductive biology.
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Specifications

Form

Liquid

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General info

Function

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Sequence similarities

Belongs to the glycosyl hydrolase 56 family.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
See full target information SPAM1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 60:1431-1441 PubMed30947333

2019

PH20 Inhibits TGFβ1-Induced Differentiation of Perimysial Orbital Fibroblasts via Hyaluronan-CD44 Pathway in Thyroid-Associated Ophthalmopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqi Ma,Hui Ren,Binbin Xu,Yun Cheng,Lu Gan,Rui Zhang,Jihong Wu,Jiang Qian
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