Recombinant human SPAM1 protein is a Human Fragment protein, in the 36 to 482 aa range, expressed in HEK 293, with >92% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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- Cell 175:117-132.e212018Extracellular Matrix Remodeling Regulates Glucose Metabolism through TXNIP Destabilization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWilliam J Sullivan,Peter J Mullen,Ernst W Schmid,Aimee Flores,Milica Momcilovic,Mark S Sharpley,David Jelinek,Andrew E Whiteley,Matthew B Maxwell,Blake R Wilde,Utpal Banerjee,Hilary A Coller,David B Shackelford,Daniel Braas,Donald E Ayer,Thomas Q de Aguiar Vallim,William E Lowry,Heather R ChristofkPubMed 30197082
Key facts
- Purity
- >92% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
L N F R A P P V I P N V P F L W A W N A P S E F C L G K F D E P L D M S L F S F I G S P R I N A T G Q G V T I F Y V D R L G Y Y P Y I D S I T G V T V N G G I P Q K I S L Q D H L D K A K K D I T F Y M P V D N L G M A V I D W E E W R P T W A R N W K P K D V Y K N R S I E L V Q Q Q N V Q L S L T E A T E K A K Q E F E K A G K D F L V E T I K L G K L L R P N H L W G Y Y L F P D C Y N H H Y K K P G Y N G S C F N V E I K R N D D L S W L W N E S T A L Y P S I Y L N T Q Q S P V A A T L Y V R N R V R E A I R V S K I P D A K S P L P V F A Y T R I V F T D Q V L K F L S Q D E L V Y T F G E T V A L G A S G I V I W G T L S I M R S M K S C L L L D N Y M E T I L N P Y I I N V T L A A K M C S Q V L C Q E Q G V C I R K N W N S S D Y L H L N P D N F A I Q L E K G G K F T V R G K P T L E D L E Q F S E K F Y C S C Y S T L S C K E K A D V K D T D A V D V C I A D G V C I D A F L K P P M E T E E P Q I F Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes DTT-reduced Protein migrates as 64-66 kDa due to glycosylation. |
Associated Products
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Target data
Function
Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Alternative names
HYAL3, PH20, SPAM1, Hyaluronidase PH-20, Hyal-PH20, Hyaluronoglucosaminidase PH-20, Sperm adhesion molecule 1, Sperm surface protein PH-20
Recommended products
Recombinant human SPAM1 protein is a Human Fragment protein, in the 36 to 482 aa range, expressed in HEK 293, with >92% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >92% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- Yes
- Biological activity
- The activity of ab174000 is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is >40,000 U/mg. Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 ml reaction mixture.
- Accession
- P38567-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute with 20% glycerol to a concentration of 100 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 5% Trehalose, 0.61% Tris, 0.58% Sodium chloride
Sequence info
Amino acid sequence
- L N F R A P P V I P N V P F L W A W N A P S E F C L G K F D E P L D M S L F S F I G S P R I N A T G Q G V T I F Y V D R L G Y Y P Y I D S I T G V T V N G G I P Q K I S L Q D H L D K A K K D I T F Y M P V D N L G M A V I D W E E W R P T W A R N W K P K D V Y K N R S I E L V Q Q Q N V Q L S L T E A T E K A K Q E F E K A G K D F L V E T I K L G K L L R P N H L W G Y Y L F P D C Y N H H Y K K P G Y N G S C F N V E I K R N D D L S W L W N E S T A L Y P S I Y L N T Q Q S P V A A T L Y V R N R V R E A I R V S K I P D A K S P L P V F A Y T R I V F T D Q V L K F L S Q D E L V Y T F G E T V A L G A S G I V I W G T L S I M R S M K S C L L L D N Y M E T I L N P Y I I N V T L A A K M C S Q V L C Q E Q G V C I R K N W N S S D Y L H L N P D N F A I Q L E K G G K F T V R G K P T L E D L E Q F S E K F Y C S C Y S T L S C K E K A D V K D T D A V D V C I A D G V C I D A F L K P P M E T E E P Q I F Y
- Accession
- P38567
- Protein length
- Fragment
- Predicted molecular weight
- 52.1 kDa
- Amino acids
- 36 to 482
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
- Sequence similarities
Belongs to the glycosyl hydrolase 56 family.
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Reconstitute for long term storage
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product was previously labelled as Hyaluronidase PH20. The protein migrates as 64-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SPAM1 also known as sperm adhesion molecule 1 or PH-20 refers to a glycoprotein found on the sperm cell surface. Its molecular mass is approximately 64 kDa. This protein is primarily expressed in the testes and epididymis where it plays a role in sperm maturation. SPAM1 possesses hyaluronidase activity breaking down hyaluronic acid present in the cumulus cell matrix of the female reproductive tract during fertilization. It aids sperm in penetrating the egg's outer layers which is important for successful fertilization.
- Biological function summary
SPAM1 participates in sperm penetration through the zona pellucida an essential step in fertilization. It acts independently facilitating sperm passage by degrading surrounding extracellular matrices. SPAM1 while not part of a larger protein complex interacts directly with the female reproductive tract's biochemical structures. These interactions ensure an efficient sperm-vs-egg recognition process that aids in sperm-egg fusion. Its enzymatic activity is central to this sperm-egg binding and membrane fusion.
- Pathways
SPAM1 contributes significantly to the fertilization pathway specifically the Acrosome Reaction. It interacts indirectly with proteins like ZP3 a zona pellucida protein that triggers the acrosome reaction necessary for sperm to penetrate the ovum. Additionally SPAM1 plays a minor role in the hyaluronan-mediated motility pathway interacting faintly with the extracellular matrix components to enhance sperm motility and penetration.
- Associated diseases and disorders
SPAM1 has been implicated in certain infertility cases particularly those involving the failure of sperm to penetrate the egg successfully. This condition might relate to defects or alterations in SPAM1 activity or expression. Moreover some studies suggest a possible link between SPAM1 and testicular cancer where aberrant expression could influence tumorigenesis. SPAM1 anomalies may associate with other proteins like EZH2 involved in various cancers suggesting broader functional implications beyond reproductive biology.
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1 product image
SDS-PAGE - Recombinant human SPAM1 protein (ab174000)
SDS-PAGE analysis of reduced ab174000 stained overnight with Coomassie Blue.
DTT-reduced Protein migrates as 64-66 kDa due to glycosylation.
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