Recombinant human SPARC protein (Active) is a Human Fragment protein, in the 18 to 297 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.
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Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, HPLC
- Biologically active
- Yes
Amino acid sequence
A P Q Q E A L P D E T E V V E E T V A E V T E V S V G A N P V Q V E V G E F D D G A E E T E E E V V A E N P C Q N H H C K H G K V C E L D E N N T P M C V C Q D P T S C P A P I G E F E K V C S N D N K T F D S S C H F F A T K C T L E G T K K G H K L H L D Y I G P C K Y I P P C L D S E L T E F P L R M R D W L K N V L V T L Y E R D E D N N L L T E K Q K L R V K K I H E N E K R L E A G D H P V E L L A R D F E K N Y N M Y I F P V H W Q F G Q L D Q H P I D G Y L S H T E L A P L R A P L I P M E H C T T R F F E T C D L D N D K Y I A L D E W A G C F G I K Q K D I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Alternative names
ON, SPARC, Basement-membrane protein 40, Osteonectin, Secreted protein acidic and rich in cysteine, BM-40
Recommended products
Recombinant human SPARC protein (Active) is a Human Fragment protein, in the 18 to 297 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.
Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, HPLC
- Biologically active
- Yes
- Biological activity
- Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 ug/mL, corresponding to a specific activity of >333 IU/mg.
- Accession
- P09486-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in water or 0.1% BSA aqueous buffer
- Concentration
- Storage buffer
Constituents: PBS
Sequence info
Amino acid sequence
- A P Q Q E A L P D E T E V V E E T V A E V T E V S V G A N P V Q V E V G E F D D G A E E T E E E V V A E N P C Q N H H C K H G K V C E L D E N N T P M C V C Q D P T S C P A P I G E F E K V C S N D N K T F D S S C H F F A T K C T L E G T K K G H K L H L D Y I G P C K Y I P P C L D S E L T E F P L R M R D W L K N V L V T L Y E R D E D N N L L T E K Q K L R V K K I H E N E K R L E A G D H P V E L L A R D F E K N Y N M Y I F P V H W Q F G Q L D Q H P I D G Y L S H T E L A P L R A P L I P M E H C T T R F F E T C D L D N D K Y I A L D E W A G C F G I K Q K D I
- Accession
- P09486
- Protein length
- Fragment
- Predicted molecular weight
- 32.7 kDa
- Amino acids
- 18 to 297
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
> 98 % by HPLC.
General info
- Function
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
- Sequence similarities
Belongs to the SPARC family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SPARC also known as Secreted Protein Acidic and Rich in Cysteine or osteonectin is a glycoprotein with a molecular mass of approximately 32 to 43 kDa. It is widely expressed in various tissues notably within the bone skin and extracellular matrix. SPARC influences cell-matrix interactions and modulates cellular functions such as proliferation and migration. The protein plays a role in tissue remodeling and wound healing by interacting with structural components of the matrix and regulating cell adhesion.
- Biological function summary
SPARC impacts processes related to cell communication and matrix dynamics. It does not form part of large complexes but functions through interactions with matrix molecules and receptors. SPARC regulates collagen fibrillogenesis and influences the bioavailability of growth factors. It further affects angiogenesis through its ability to alter cellular adhesion and spreading which impacts vascular development and repair processes.
- Pathways
SPARC integrates into regulatory cascades such as the WNT signaling and TGF-β pathways. These pathways are essential for cellular growth repair and differentiation. In the context of the TGF-β signaling pathway SPARC modulates interactions with proteins like integrins and collagens impacting fibrotic processes and matrix assembly.
- Associated diseases and disorders
Alterations in SPARC expression show associations with cancer and fibrosis. The protein functions in tumor progression where it can influence tumor cell interactions with the stroma and affect cell migration and invasion. Furthermore in fibrotic diseases SPARC regulates the deposition and organization of collagen contributing to tissue stiffening. Connections are evident between SPARC and other matrix proteins like fibronectin known for their roles in these pathological conditions.
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1 product image
SDS-PAGE - Recombinant human SPARC protein (Active) (ab243161)
SDS-PAGE analysis of ab243161
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