Recombinant Human SPARC protein (His tag) is a Human Full Length protein, in the 1 to 303 aa range, expressed in HEK 293, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M R A W I F F L L C L A G R A L A A P Q Q E A L P D E T E V V E E T V A E V T E V S V G A N P V Q V E V G E F D D G A E E T E E E V V A E N P C Q N H H C K H G K V C E L D E N N T P M C V C Q D P T S C P A P I G E F E K V C S N D N K T F D S S C H F F A T K C T L E G T K K G H K L H L D Y I G P C K Y I P P C L D S E L T E F P L R M R D W L K N V L V T L Y E R D E D N N L L T E K Q K L R V K K I H E N E K R L E A G D H P V E L L A R D F E K N Y N M Y I F P V H W Q F G Q L D Q H P I D G Y L S H T E L A P L R A P L I P M E H C T T R F F E T C D L D N D K Y I A L D E W A G C F G I K Q K D I D K D L V I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Alternative names
ON, SPARC, Basement-membrane protein 40, Osteonectin, Secreted protein acidic and rich in cysteine, BM-40
Recommended products
Recombinant Human SPARC protein (His tag) is a Human Full Length protein, in the 1 to 303 aa range, expressed in HEK 293, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P09486-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M R A W I F F L L C L A G R A L A A P Q Q E A L P D E T E V V E E T V A E V T E V S V G A N P V Q V E V G E F D D G A E E T E E E V V A E N P C Q N H H C K H G K V C E L D E N N T P M C V C Q D P T S C P A P I G E F E K V C S N D N K T F D S S C H F F A T K C T L E G T K K G H K L H L D Y I G P C K Y I P P C L D S E L T E F P L R M R D W L K N V L V T L Y E R D E D N N L L T E K Q K L R V K K I H E N E K R L E A G D H P V E L L A R D F E K N Y N M Y I F P V H W Q F G Q L D Q H P I D G Y L S H T E L A P L R A P L I P M E H C T T R F F E T C D L D N D K Y I A L D E W A G C F G I K Q K D I D K D L V I
- Accession
- P09486
- Protein length
- Full Length
- Predicted molecular weight
- 34 kDa
- Amino acids
- 1 to 303
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
- Sequence similarities
Belongs to the SPARC family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SPARC also known as Secreted Protein Acidic and Rich in Cysteine or osteonectin is a glycoprotein with a molecular mass of approximately 32 to 43 kDa. It is widely expressed in various tissues notably within the bone skin and extracellular matrix. SPARC influences cell-matrix interactions and modulates cellular functions such as proliferation and migration. The protein plays a role in tissue remodeling and wound healing by interacting with structural components of the matrix and regulating cell adhesion.
- Biological function summary
SPARC impacts processes related to cell communication and matrix dynamics. It does not form part of large complexes but functions through interactions with matrix molecules and receptors. SPARC regulates collagen fibrillogenesis and influences the bioavailability of growth factors. It further affects angiogenesis through its ability to alter cellular adhesion and spreading which impacts vascular development and repair processes.
- Pathways
SPARC integrates into regulatory cascades such as the WNT signaling and TGF-β pathways. These pathways are essential for cellular growth repair and differentiation. In the context of the TGF-β signaling pathway SPARC modulates interactions with proteins like integrins and collagens impacting fibrotic processes and matrix assembly.
- Associated diseases and disorders
Alterations in SPARC expression show associations with cancer and fibrosis. The protein functions in tumor progression where it can influence tumor cell interactions with the stroma and affect cell migration and invasion. Furthermore in fibrotic diseases SPARC regulates the deposition and organization of collagen contributing to tissue stiffening. Connections are evident between SPARC and other matrix proteins like fibronectin known for their roles in these pathological conditions.
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1 product image
SDS-PAGE - Recombinant Human SPARC protein (His tag) (ab276339)
SDS-PAGE analysis of ab276339
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