Recombinant Human Spastin protein is a Human Fragment protein, in the 200 to 304 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Publications
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- Neural regeneration research 14:1271-12792019Effect of exogenous spastin combined with polyethylene glycol on sciatic nerve injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao-Fa Lin,Zheng Xie,Jun Zhou,Hui-Hao Chen,Wan-Wan Shao,Hao-Dong LinPubMed 30804259
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
P V L P F S K S Q T D V Y N D S T N L A C R N G H L Q S E S G A V P K R K D P L T H T S N S L P R S K T V M K T G S A G L S G H H R A P S Y S G L S M V S G V K Q G S G P A P T T H K G T P K T N R T N K P S T P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed:11809724, PubMed:15716377, PubMed:16219033, PubMed:17389232, PubMed:20530212, PubMed:22637577, PubMed:26875866). Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Severing activity is not dependent on tubulin acetylation or detyrosination (PubMed:26875866). Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex (PubMed:19000169, PubMed:21310966, PubMed:26040712). Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex (PubMed:21310966). Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed:26040712). Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling (PubMed:23897888). Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing (PubMed:23897888). Probably plays a role in axon growth and the formation of axonal branches (PubMed:15716377). Isoform 1. Involved in lipid metabolism by regulating the size and distribution of lipid droplets.
Alternative names
ADPSP, FSP2, KIAA1083, SPG4, SPAST, Spastin, Spastic paraplegia 4 protein
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Recombinant Human Spastin protein is a Human Fragment protein, in the 200 to 304 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q9UBP0-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- P V L P F S K S Q T D V Y N D S T N L A C R N G H L Q S E S G A V P K R K D P L T H T S N S L P R S K T V M K T G S A G L S G H H R A P S Y S G L S M V S G V K Q G S G P A P T T H K G T P K T N R T N K P S T P
- Accession
- Q9UBP0
- Protein length
- Fragment
- Predicted molecular weight
- 37.29 kDa
- Amino acids
- 200 to 304
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed:11809724, PubMed:15716377, PubMed:16219033, PubMed:17389232, PubMed:20530212, PubMed:22637577, PubMed:26875866). Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Severing activity is not dependent on tubulin acetylation or detyrosination (PubMed:26875866). Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex (PubMed:19000169, PubMed:21310966, PubMed:26040712). Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex (PubMed:21310966). Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed:26040712). Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling (PubMed:23897888). Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing (PubMed:23897888). Probably plays a role in axon growth and the formation of axonal branches (PubMed:15716377).
- Sequence similarities
Belongs to the AAA ATPase family. Spastin subfamily.
- Subcellular localisation
- Cytoskeleton, Microtubule organizing center, Centrosome, Nucleus, Spindle, Nucleus membrane, Endosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Spastin also known as SPG4 is a microtubule-severing ATPase that plays an important role in cellular mechanics by cutting microtubules in cells. It weighs approximately 68 kDa and is present mainly in neural tissue but can also be found in other tissues. The protein's localization includes the cytoplasm and is associated with centrosomes and subcellular regions with dense microtubule networks.
- Biological function summary
Spastin participates in regulating microtubule dynamics. It plays a critical role in axonal growth and maintenance by modulating microtubule lengths and branching. Spastin does not function alone; it forms part of the AAA protein family functioning in significant processes like neurogenesis and neural maintenance. Its activity affects neural cell structure and transport by influencing cytoskeletal rearrangement.
- Pathways
Any disturbances in Spastin function can impact the microtubule dynamics pathway and the axonal transport pathway. Spastin interacts closely with proteins such as Tubulin and Katanin which are also involved in microtubule severing and organization. Proper Spastin function is necessary to maintain intracellular transport and signal transduction critical for neural cell health and function.
- Associated diseases and disorders
Alterations in Spastin activity lead to neurodegenerative conditions like Hereditary Spastic Paraplegia (HSP). This disorder often relates to mutations in the SPG4 gene which encodes Spastin affecting motor neuron functions. In HSP disrupted Spastin function correlates with deficiencies in proteins such as Tubulin exacerbating neuroaxonal deficits and influencing disease progression.
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1 product image
SDS-PAGE - Recombinant Human Spastin protein (ab152700)
12.5% SDS-PAGE analysis of ab152700 stained with Coomassie Blue.
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