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AB174402

Recombinant Human SPF45 protein (His tag N-Terminus)

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Recombinant Human SPF45 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 401 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

SPF45, RBM17, Splicing factor 45, 45 kDa-splicing factor, RNA-binding motif protein 17

1 Images
SDS-PAGE - Recombinant Human SPF45 protein (His tag N-Terminus) (AB174402)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SPF45 protein (His tag N-Terminus) (AB174402)

15% SDS-PAGE analysis of ab174402 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q96I25

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as RBM17
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSLYDDLGVETSDSKTEGWSKNFKLLQSQLQVKKAALTQAKSQRTKQSTVLAPVIDLKRGGSSDDRQIVDTPPHVAAGLKDPVPSGFSAGEVLIPLADEYDPMFPNDYEKVVKRQREERQRQRELERQKEIEEREKRRKDRHEASGFARRPDPDSDEDEDYERERRKRSMGGAAIAPPTSLVEKDKELPRDFPYEEDSRPRSQSSKAAIPPPVYEEQDRPRSPTGPSNSFLANMGGTVAHKIMQKYGFREGQGLGKHEQGLSTALSVEKTSKRGGKIIVGDATEKDASKKSDSNPLTEILKCPTKVVLLRNMVGAGEVDEDLEVETKEECEKYGKVGKCVIFEIPGAPDDEAVRIFLEFERVESAIKAVVDLNGRYFGGRVVKACFYNLDKFRVLDLAEQV","proteinLength":"Full Length","predictedMolecularWeight":"47.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":401,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96I25","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPF45 also known as RBM17 (RNA binding motif protein 17) is a protein that plays an essential role in pre-mRNA splicing. With a molecular weight of approximately 45 kDa SPF45 is expressed widely in human tissues with significant levels in the testes ovaries and other proliferative tissues. The protein consists of an RNA recognition motif which facilitates its binding to RNA molecules during the splicing process. Through these interactions SPF45 contributes significantly to the modification and maturation of pre-mRNA molecules.
Biological function summary

SPF45 interacts with components of the spliceosome a large and dynamic ribonucleoprotein complex essential for the removal of introns from pre-mRNA. This protein serves as an activator in the alternative splicing of transcripts thereby influencing gene expression patterns. SPF45's binding with other spliceosome-associated proteins and its participation in splicing regulation highlight its role in generating diverse protein isoforms which are important for cell differentiation and development. The interactions within the spliceosomal complex mark SPF45 as a significant player in mRNA biogenesis.

Pathways

SPF45 participates prominently in the regulation of the spliceosome and consequently in the overall RNA processing pathway. By modulating alternative splicing SPF45 affects pathways such as signal transduction and cell cycle control. SPF45 closely interacts with other splicing factors like SF1 and U2AF65 forming important nodes in the network of splicing regulation. Its activity in spliceosome assembly and function is integral influencing various downstream pathways essential for cellular response and adaptation.

SPF45 alterations have been implicated in cancer and neurodegenerative diseases. SPF45's regulation of alternative splicing can lead to aberrant gene expression when disrupted contributing to tumorigenesis. Increased or altered expression levels are observed in several cancers linking it to oncogenic pathways. Additionally in neurodegenerative disorders SPF45's role in splicing regulation intersects with proteins such as TDP-43 impacting neuronal health and function. Its intricate involvement highlights the importance of SPF45 in both normal cellular processes and pathological conditions.
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Specifications

Form

Liquid

Additional notes

ab174402 is purified using conventional chromatography techniques.

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General info

Function

Splice factor that binds to the single-stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Splice factor that binds to the single-stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
See full target information RBM17
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