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AB128446

Recombinant Human spindlin 1 protein

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Recombinant Human spindlin 1 protein is a Human Full Length protein, in the 1 to 262 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

OCR, SPIN, SPIN1, Spindlin-1, Ovarian cancer-related protein, Spindlin1

1 Images
SDS-PAGE - Recombinant Human spindlin 1 protein (AB128446)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human spindlin 1 protein (AB128446)

15% SDS-PAGE analysis of ab128446 (3ug).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9Y657

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 32% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMKTPFGKTPGQRSRADAGHAGVSANMMKKRTSHKKHRSSVGPSKPVSQPRRNIVGCRIQHGWKEGNGPVTQWKGTVLDQVPVNPSLYLIKYDGFDCVYGLELNKDERVSALEVLPDRVATSRISDAHLADTMIGKAVEHMFETEDGSKDEWRGMVLARAPVMNTWFYITYEKDPVLYMYQLLDDYKEGDLRIMPDSNDSPPAEREPGEVVDSLVGKQVEYAKEDGSKRTGMVIHQVEAKPSVYFIKFDDDFHIYVYDLVKTS","proteinLength":"Full Length","predictedMolecularWeight":"32 kDa","actualMolecularWeight":null,"aminoAcidEnd":262,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y657","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spindlin 1 also known as SPIN1 is a protein that functions as a histone code reader. It weighs approximately 29 kDa and is highly expressed in the testes ovaries and certain cancer cell lines. Spindlin 1 has three tandem Tudor domains which allow it to interact with methylated histones specifically histone H3. These interactions enable SPIN1 to regulate gene expression by binding to modified histones and influencing the transcriptional machinery.
Biological function summary

SPIN1 acts as a regulator of gene expression by interacting with epigenetic markers on histones. It is part of the transcriptional regulation complex which includes other proteins involved in chromatin remodeling and gene activation. Its role in binding to trimethylated lysine 4 on histone H3 (H3K4me3) highlights its impact on transcriptional regulation processes. This interaction is important for maintaining the pluripotency of stem cells and possibly influencing cell differentiation.

Pathways

SPIN1 functions mainly within the epigenetic regulation and gene expression pathways. It affects the histone modification pathway and plays an important role in regulating the transcriptional activation process. SPIN1's relationship with other proteins like the histone methyltransferase SETD3 and components of the chromatin-remodeling complex defines its role in gene regulation mechanisms. Together these proteins determine chromatin structure and gene activity impacting transcriptional outcomes.

SPIN1 has associations with cancer particularly ovarian and testicular cancers. This link arises from its overexpression which can promote tumorigenesis by altering gene expression profiles in cancerous cells. SPIN1 also interacts with oncogenic pathways involving proteins like MYC further implicating it in cancer development. Additionally its abnormal functioning can contribute to infertility issues due to its vital role in reproductive tissues with links to proteins involved in spermatogenesis and oogenesis.
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Specifications

Form

Liquid

Additional notes

ab128446 was purified using conventional chromatography.

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General info

Function

Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway downstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway (PubMed : 24589551). Overexpression induces metaphase arrest and chromosomal instability. Localizes to active rDNA loci and promotes the expression of rRNA genes (PubMed : 21960006). May play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I : may act by regulating maternal transcripts to control meiotic resumption.

Sequence similarities

Belongs to the SPIN/STSY family.

Post-translational modifications

Phosphorylated during oocyte meiotic maturation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway downstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway (PubMed : 24589551). Overexpression induces metaphase arrest and chromosomal instability. Localizes to active rDNA loci and promotes the expression of rRNA genes (PubMed : 21960006). May play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I : may act by regulating maternal transcripts to control meiotic resumption.
See full target information SPIN1
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