JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB167864

Recombinant Human SPOC1 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human SPOC1 protein is a Human Full Length protein, in the 1 to 300 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PHD finger protein 13, Survival time-associated PHD finger protein in ovarian cancer 1, SPOC1, PHF13

1 Images
SDS-PAGE - Recombinant Human SPOC1 protein (AB167864)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SPOC1 protein (AB167864)

15% SDS-PAGE analysis of ab167864 (3µg)

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q86YI8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 1.7% Imidazole Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris-HCl buffer, 0.03% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Previously labelled as PHF13.
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMDSDSCAAAFHPEEYSPSCKRRRTVEDFNKFCTFVLAYAGYIPYPKEELPLRSSPSPANSTAGTIDSDGWDAGFSDIASSVPLPVSDRCFSHLQPTLLQRAKPSNFLLDRKKTDKLKKKKKRKRRDSDAPGKEGYRGGLLKLEAADPYVETPTSPTLQDIPQAPSDPCSGWDSDTPSSGSCATVSPDQVKEIKTEGKRTIVRQGKQVVFRDEDSTGNDEDIMVDSDDDSWDLVTCFCMKPFAGRPMIECNECHTWIHLSCAKIRKSNVPEVFVCQKCRDSKFDIRRSNRSRTGSRKLFLD","proteinLength":"Full Length","predictedMolecularWeight":"36 kDa","actualMolecularWeight":null,"aminoAcidEnd":300,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q86YI8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPOC1 also known as Scaffold/Matrix Attachment Region-Binding Protein 1 (SPMARBP1) is a nuclear protein with a molecular mass of approximately 27 kDa. It expresses itself predominantly in nucleated cells such as lymphocytes and fibroblasts. The protein contains a SPOC domain that facilitates protein-protein interactions playing an important role in chromatin structure. SPOC1 binds to chromatin and contributes to chromatin compaction influencing the transcriptional activity within the nucleous.
Biological function summary

SPOC1 functions as a chromatin-associated protein that aids in genomic stability and regulation of transcription. It is involved in forming chromatin loops. SPOC1 can interact with other nuclear proteins like histones modifying chromatin dynamics. Its role extends to safeguarding genomic integrity by stabilizing chromatin architecture ensuring proper DNA repair and promoting efficient replication of DNA. Additionally the protein helps in cellular response to genotoxic stress integrating signals to modulate chromatin structure accordingly.

Pathways

SPOC1 actively participates in DNA damage repair and chromatin remodeling pathways. In the context of DNA damage response the protein associates with the repair pathways ensuring high-fidelity DNA replication and repair. SPOC1 works alongside proteins like ATM (Ataxia Telangiectasia Mutated) and ATR (ATM and Rad3-related) in pathways that maintain genomic stability. These interactions highlight its role in protecting cellular integrity and preventing mutation accumulation during the cell cycle.

Altered expression or mutations of SPOC1 have links to cancer and autoimmune diseases. The protein may promote oncogenesis when its regulation in chromatin dynamics is disrupted leading to abnormal gene expression patterns and genomic instability. Changes in SPOC1 function potentially contribute to malignancies by interfering with normal cell cycle regulation. In autoimmune disorders dysregulation of SPOC1 might interact with proteins like p53 affecting immune cell proliferation and response. Understanding the role of SPOC1 in these diseases offers insights into therapeutic target development.
style
left-column

Specifications

Form

Liquid

Additional notes

ab167864 was purified using conventional chromatography.

style
left-column

General info

Function

Modulates chromatin structure and DNA damage response by regulating key determinants of chromatin compaction and DNA damage response (PubMed : 19638409). Binds H3K4me3-containing chromatin and promotes DNA condensation by recruiting corepressors such as TRIM28 and H3K9 methyltransferase SETDB1 (PubMed : 23034801). Required for normal chromosome condensation during the early stages of mitosis. Required for normal chromosome separation during mitosis (PubMed : 19638409). Increases both chromatin-associated levels and activity of H3K9 methyltransferases, such as SETDB1, thus enhancing H3K9 trimethylation (PubMed : 23034801). Essential for testicular stem-cell differentiation and sustained spermatogenesis (By similarity).

Post-translational modifications

Subject to proteasomal degradation. Stable when bound to chromatin. The soluble form is rapidly degraded.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Modulates chromatin structure and DNA damage response by regulating key determinants of chromatin compaction and DNA damage response (PubMed : 19638409). Binds H3K4me3-containing chromatin and promotes DNA condensation by recruiting corepressors such as TRIM28 and H3K9 methyltransferase SETDB1 (PubMed : 23034801). Required for normal chromosome condensation during the early stages of mitosis. Required for normal chromosome separation during mitosis (PubMed : 19638409). Increases both chromatin-associated levels and activity of H3K9 methyltransferases, such as SETDB1, thus enhancing H3K9 trimethylation (PubMed : 23034801). Essential for testicular stem-cell differentiation and sustained spermatogenesis (By similarity).
See full target information PHF13
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Secondary Antibodies

AB102199

Rabbit Anti-Rat IgG+IgM+IgA H&L (HRP)

  • 0
  • 0
Secondary Antibodies

AB150095

Goat Anti-Rabbit IgM mu chain (Alexa Fluor® 647)

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgM mu chain (Alexa Fluor® 647) (AB150095)

  • 0
  • 0
Proteins & Peptides

AB171689

Recombinant Human ZNF346 protein

SDS-PAGE - Recombinant Human ZNF346 protein (AB171689)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com