Recombinant Human Sprouty 4/Spry-4 protein (denatured) is a Human Full Length protein, in the 1 to 299 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M E P P I P Q S A P L T P N S V M V Q P L L D S R M S H S R L Q H P L T I L P I D Q V K T S H V E N D Y I D N P S L A L T T G P K R T R G G A P E L A P T P A R C D Q D V T H H W I S F S G R P S S V S S S S S T S S D Q R L L D H M A P P P V A D Q A S P R A V R I Q P K V V H C Q P L D L K G P A V P P E L D K H F L L C E A C G K C K C K E C A S P R T L P S C W V C N Q E C L C S A Q T L V N Y G T C M C L V Q G I F Y H C T N E D D E G S C A D H P C S C S R S N C C A R W S F M G A L S V V L P C L L C Y L P A T G C V K L A Q R G Y D R L R R P G C R C K H T N S V I C K A A S G D A K T S R P D K P F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras (PubMed:12027893). Probably impairs the formation of GTP-Ras (PubMed:12027893). Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 (PubMed:12717443). Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin (PubMed:15584898).
Alternative names
Protein sprouty homolog 4, Spry-4, SPRY4
Recommended products
Recombinant Human Sprouty 4/Spry-4 protein (denatured) is a Human Full Length protein, in the 1 to 299 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9C004-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M E P P I P Q S A P L T P N S V M V Q P L L D S R M S H S R L Q H P L T I L P I D Q V K T S H V E N D Y I D N P S L A L T T G P K R T R G G A P E L A P T P A R C D Q D V T H H W I S F S G R P S S V S S S S S T S S D Q R L L D H M A P P P V A D Q A S P R A V R I Q P K V V H C Q P L D L K G P A V P P E L D K H F L L C E A C G K C K C K E C A S P R T L P S C W V C N Q E C L C S A Q T L V N Y G T C M C L V Q G I F Y H C T N E D D E G S C A D H P C S C S R S N C C A R W S F M G A L S V V L P C L L C Y L P A T G C V K L A Q R G Y D R L R R P G C R C K H T N S V I C K A A S G D A K T S R P D K P F
- Accession
- Q9C004
- Protein length
- Full Length
- Predicted molecular weight
- 34.9 kDa
- Amino acids
- 1 to 299
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras (PubMed:12027893). Probably impairs the formation of GTP-Ras (PubMed:12027893). Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 (PubMed:12717443). Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin (PubMed:15584898).
- Sequence similarities
Belongs to the sprouty family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as Sprouty 4.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Spry-4 protein also known as Sprouty 4 is an inhibitor of receptor tyrosine kinase signaling pathways. It weighs approximately 35 kDa and plays a role in modulating fibroblast growth factor (FGF) and epidermal growth factor (EGF) signaling. Spry-4 is expressed in various tissues including the brain lungs and kidneys. Its expression varies during different developmental stages and under different physiological conditions.
- Biological function summary
Sprouty family proteins such as Spry-4 negatively regulate cell growth differentiation and angiogenesis. This protein functions as part of a negative feedback loop in growth factor-mediated signaling. By modulating signaling Spry-4 helps maintain balanced cellular responses ensuring cells do not undergo uncontrolled proliferation or differentiation. Spry-4's regulatory role is important in tissue development and repair.
- Pathways
Spry-4 plays a significant role in the MAPK/ERK and PI3K/AKT pathways. In these pathways Spry-4 interacts with other proteins like RAF1 and Erk. Its inhibitory effects prevent excessive activation of these pathways which are essential for processes like cell division and survival. Spry-4's ability to modulate these pathways determines how cells respond to external stimuli maintaining cellular homeostasis.
- Associated diseases and disorders
Spry-4's dysregulation has been linked to cancer and congenital disorders. For example altered expression of Spry-4 can lead to tumorigenesis since the balance in growth factor signaling is disrupted. In cancer Spry-4 often interacts with other regulatory proteins such as SOS1 which further modulates signaling pathways important for tumor growth. In congenital disorders mutations in Spry-4 or related pathways affect normal tissue development leading to developmental anomalies.
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1 product image
SDS-PAGE - Recombinant Human Sprouty 4/Spry-4 protein (denatured) (ab171684)
15% SDS-PAGE analysis of ab171684 (3µg).
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