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AB190389

Recombinant human Sps1/Ste20 related kinase 4 protein

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Recombinant human Sps1/Ste20 related kinase 4 protein is a Human Fragment protein, in the 941 to 1328 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

RCK, YSK4, MAP3K19, Mitogen-activated protein kinase kinase kinase 19, SPS1/STE20-related protein kinase YSK4

4 Images
Functional Studies - Recombinant human Sps1/Ste20 related kinase 4 protein (AB190389)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Sps1/Ste20 related kinase 4 protein (AB190389)

Kinase assay showing the specific activity of ab190389 as 38 nmol/min/mg.

Functional Studies - Recombinant human Sps1/Ste20 related kinase 4 protein (AB190389)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Sps1/Ste20 related kinase 4 protein (AB190389)

The specific activity of Sps1/Ste20 related kinase 4 (ab190389) was determined to be 44 nmol/min/mg as per activity assay protocol.

SDS-PAGE - Recombinant human Sps1/Ste20 related kinase 4 protein (AB190389)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Sps1/Ste20 related kinase 4 protein (AB190389)

SDS-PAGE analysis of ab190389.

SDS-PAGE - Recombinant human Sps1/Ste20 related kinase 4 protein (AB190389)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Sps1/Ste20 related kinase 4 protein (AB190389)

SDS PAGE analysis of ab190389

Key facts

Purity

>80% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS, WB

applications

Biologically active

Yes

Biological activity

The specific activity of ab190389 was determined to be 38 nmol/min/mg.

Accession

Q56UN5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.1% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Complementary Products

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Primary Antibodies

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Primary Antibodies

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"linker":null,"sequence":"MFGKTLSGTNSISQEIMDSVNNEELTDELLGCLAAELLALDEKDNNSCQKMANETDPENLNLVLRWRGSTPKEMGRETTKVKIQRHSSGLRIYDREEKFLISNEKKIFSENSLKSEEPILWTKGEILGKGAYGTVYCGLTSQGQLIAVKQVALDTSNKLAAEKEYRKLQEEVDLLKALKHVNIVAYLGTCLQENTVSIFMEFVPGGSISSIINRFGPLPEMVFCKYTKQILQGVAYLHENCVVHRDIKGNNVMLMPTGIIKLIDFGCARRLAWAGLNGTHSDMLKSMHGTPYWMAPEVINESGYGRKSDIWSIGCTVFEMATGKPPLASMDRMAAMFYIGAHRGLMPPLPDHFSENAADFVRMCLTRDQHERPSALQLLKHSFLERSH","proteinLength":"Fragment","predictedMolecularWeight":"83 kDa","actualMolecularWeight":null,"aminoAcidEnd":1328,"aminoAcidStart":941,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q56UN5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sps1/Ste20 related kinase 4 also known as STK4 or MST1 is a serine/threonine kinase with a molecular mass of approximately 56 kDa. This kinase belongs to the Germinal Center Kinase (GCK) group of the STE20-like kinase family and it plays a significant role in signal transduction processes. STK4 is broadly expressed with prominent presence in tissues like the liver and pancreas. The protein localizes both in the cytoplasm and nucleus and is known to translocate in response to stress signals.
Biological function summary

This kinase helps regulate apoptosis and cell proliferation. As a part of the Hippo signaling pathway STK4/MST1 operates within a cascade of interactions involving multiple cellular proteins. It forms a complex with other proteins such as SAV1 and LATS1/2 to execute control over organ size by modulating cell growth and apoptosis. Through these interactions STK4 maintains cellular homeostasis and prevents abnormal cellular developments such as overgrowth.

Pathways

STK4 plays an integral part in the Hippo signaling and MAPK pathways. Within the Hippo pathway it partners with MST2 to phosphorylate downstream effectors which impacts transcription regulation and ultimately cellular proliferation and apoptosis. In the MAPK pathway STK4’s activities interface with those of the YAP and TAZ proteins further linking it to cellular signaling processes that control cell fate decisions.

Aberrant STK4 activity connects to cancer and neurodegenerative disorders. Its dysfunctional signaling in the Hippo pathway can lead to tumorigenesis especially when downregulation occurs reducing its inhibitory control over cell growth. In the context of neurodegenerative disorders altered STK4 activity associates with conditions like Alzheimer's disease where its interaction with tau protein influences tau phosphorylation and aggregation both hallmarks of the disease.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

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Product protocols

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Target data

See full target information MAP3K19
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Product promise

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