Recombinant Human SQSTM1 / p62 protein is a Human Full Length protein, in the 1 to 440 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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- Clinical and experimental pharmacology & physiology 50:431-4422023LncRNA PRKCQ-AS1 regulates paclitaxel resistance in triple-negative breast cancer cells through miR-361-5p/PIK3C3 mediated autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShurong Zheng,Weida Fu,Qidi Huang,Jieyu Zhou,Kangkang Lu,Junwei Gu,Ruimin Ma,Guilong GuoPubMed 36732923
- PloS one 14:e02104742019Formation of high molecular weight p62 by CORM-3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesToshihiko Aki et. al.PubMed 30620762
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A S L T V K A Y L L G K E D A A R E I R R F S F C C S P E P E A E A E A A A G P G P C E R L L S R V A A L F P A L R P G G F Q A H Y R D E D G D L V A F S S D E E L T M A M S Y V K D D I F R I Y I K E K K E C R R D H R P P C A Q E A P R N M V H P N V I C D G C N G P V V G T R Y K C S V C P D Y D L C S V C E G K G L H R G H T K L A F P S P F G H L S E G F S H S R W L R K V K H G H F G W P G W E M G P P G N W S P R P P R A G E A R P G P T A E S A S G P S E D P S V N F L K N V G E S V A A A L S P L G I E V D I D V E H G G K R S R L T P V S P E S S S T E E K S S S Q P S S C C S D P S K P G G N V E G A T Q S L A E Q M R K I A L E S E G R P E E Q M E S D N C S G G D D D W T H L S S K E V D P S T G E L Q S L Q M P E S E G P S S L D P S Q E G P T G L K E A A L Y P H L P P E A D P R L I E S L S Q M L S M G F S D E G G W L T R L L Q T K N Y D I G A A L D T I Q Y S K H P P P L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
The protein expressed by the SQSTM1 gene is an autophagy receptor essential for selective macroautophagy (aggrephagy). It acts as a bridge between polyubiquitinated cargo and autophagosomes, interacting with both the cargo and an autophagy modifier from the MAP1 LC3 family. Alongside WDFY3, it plays a role in forming and autophagically degrading cytoplasmic ubiquitin-containing inclusions and recruiting ubiquitinated proteins to nuclear PML bodies. SQSTM1 may regulate NFKB1 activation by TNF-alpha, nerve growth factor (NGF), and interleukin-1, and may influence titin/TTN signaling in muscle cells. It may also affect signaling cascades via ubiquitination and be involved in cell differentiation, apoptosis, immune response, and regulation of potassium channels. The protein is involved in endosome organization by retaining vesicles in the perinuclear cloud; ubiquitination by RNF26 allows it to attract vesicle-associated adapters, forming a molecular bridge to organize endosomal pathways. Additionally, it promotes the relocalization of Lys-63-linked ubiquitinated STING1 to autophagosomes and acts as an activator of the NFE2L2/NRF2 pathway by interacting with KEAP1, which inactivates the BCR(KEAP1) complex, resulting in nuclear accumulation of NFE2L2/NRF2 and expression of cytoprotective genes. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
ORCA, OSIL, SQSTM1, Sequestosome-1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBIAP, p60, p62
Recommended products
Recombinant Human SQSTM1 / p62 protein is a Human Full Length protein, in the 1 to 440 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q13501-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A S L T V K A Y L L G K E D A A R E I R R F S F C C S P E P E A E A E A A A G P G P C E R L L S R V A A L F P A L R P G G F Q A H Y R D E D G D L V A F S S D E E L T M A M S Y V K D D I F R I Y I K E K K E C R R D H R P P C A Q E A P R N M V H P N V I C D G C N G P V V G T R Y K C S V C P D Y D L C S V C E G K G L H R G H T K L A F P S P F G H L S E G F S H S R W L R K V K H G H F G W P G W E M G P P G N W S P R P P R A G E A R P G P T A E S A S G P S E D P S V N F L K N V G E S V A A A L S P L G I E V D I D V E H G G K R S R L T P V S P E S S S T E E K S S S Q P S S C C S D P S K P G G N V E G A T Q S L A E Q M R K I A L E S E G R P E E Q M E S D N C S G G D D D W T H L S S K E V D P S T G E L Q S L Q M P E S E G P S S L D P S Q E G P T G L K E A A L Y P H L P P E A D P R L I E S L S Q M L S M G F S D E G G W L T R L L Q T K N Y D I G A A L D T I Q Y S K H P P P L
- Accession
- Q13501
- Protein length
- Full Length
- Predicted molecular weight
- 74.14 kDa
- Amino acids
- 1 to 440
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
The protein expressed by the SQSTM1 gene is an autophagy receptor essential for selective macroautophagy (aggrephagy). It acts as a bridge between polyubiquitinated cargo and autophagosomes, interacting with both the cargo and an autophagy modifier from the MAP1 LC3 family. Alongside WDFY3, it plays a role in forming and autophagically degrading cytoplasmic ubiquitin-containing inclusions and recruiting ubiquitinated proteins to nuclear PML bodies. SQSTM1 may regulate NFKB1 activation by TNF-alpha, nerve growth factor (NGF), and interleukin-1, and may influence titin/TTN signaling in muscle cells. It may also affect signaling cascades via ubiquitination and be involved in cell differentiation, apoptosis, immune response, and regulation of potassium channels. The protein is involved in endosome organization by retaining vesicles in the perinuclear cloud; ubiquitination by RNF26 allows it to attract vesicle-associated adapters, forming a molecular bridge to organize endosomal pathways. Additionally, it promotes the relocalization of Lys-63-linked ubiquitinated STING1 to autophagosomes and acts as an activator of the NFE2L2/NRF2 pathway by interacting with KEAP1, which inactivates the BCR(KEAP1) complex, resulting in nuclear accumulation of NFE2L2/NRF2 and expression of cytoprotective genes.
This supplementary information is collated from multiple sources and compiled automatically.- Post-translational modifications
Phosphorylation at Ser-407 by ULK1 destabilizes the UBA dimer interface and increases binding affinity to ubiquitinated proteins (By similarity). Phosphorylation at Ser-407 also primes for subsequent phosphorylation at Ser-403 (By similarity). Phosphorylation at Ser-403 by CK2 or ULK1 promotes binding to ubiquitinated proteins by increasing the affinity between the UBA domain and polyubiquitin chains (PubMed:22017874, PubMed:25040165). Phosphorylation at Ser-403 by ULK1 is stimulated by SESN2 (PubMed:25040165). Phosphorylated at Ser-403 by TBK1, leading to promote relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes (PubMed:29496741). Phosphorylation at Ser-349 by ULK1 promotes interaction with KEAP1 and inactivation of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes (PubMed:37306101). Phosphorylated in vitro by TTN (PubMed:15802564).
- Subcellular localisation
- Late endosome, Lysosome, Nucleus, PML body
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human SQSTM1 / p62 protein (ab132366)
12.5% SDS-PAGE analysis of ab132366 stained with Coomassie Blue.
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