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AB114675

Recombinant Human SRSF3 protein (GST tag N-Terminus)

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(1 Publication)

Recombinant Human SRSF3 protein (GST tag N-Terminus) is a Human Fragment protein, in the 1 to 85 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

SFRS3, SRP20, SRSF3, Serine/arginine-rich splicing factor 3, Pre-mRNA-splicing factor SRP20

1 Images
SDS-PAGE - Recombinant Human SRSF3 protein (GST tag N-Terminus) (AB114675)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SRSF3 protein (GST tag N-Terminus) (AB114675)

SDS-PAGE analysis of ab114675 on a 12.5% gel stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

P84103

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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AB101049

Recombinant Human Calneuron 1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Calneuron 1 protein (His tag N-Terminus) (AB101049)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB198291

Anti-SRSF3 antibody [EPR16976]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SRSF3 antibody [EPR16976] (AB198291)

  • 5
  • 6
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as SFRS3.
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Sequence info

[{"sequence":"MHRDSCPLDCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEK","proteinLength":"Fragment","predictedMolecularWeight":"34.98 kDa","actualMolecularWeight":null,"aminoAcidEnd":85,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P84103","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Splicing factor, which binds the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing (PubMed : 17036044, PubMed : 26876937, PubMed : 32440474). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons (PubMed : 26876937). Also functions as an adapter involved in mRNA nuclear export (PubMed : 11336712, PubMed : 18364396, PubMed : 28984244). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity (PubMed : 11336712, PubMed : 18364396). Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1 : interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed : 28984244).

Sequence similarities

Belongs to the splicing factor SR family.

Post-translational modifications

Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Splicing factor, which binds the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing (PubMed : 17036044, PubMed : 26876937, PubMed : 32440474). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons (PubMed : 26876937). Also functions as an adapter involved in mRNA nuclear export (PubMed : 11336712, PubMed : 18364396, PubMed : 28984244). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity (PubMed : 11336712, PubMed : 18364396). Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1 : interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed : 28984244).
See full target information SRSF3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 18:3696-707 PubMed19602482

2009

Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Vânia Gonçalves,Paulo Matos,Peter Jordan
View all publications
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