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AB140730

Recombinant Human SSBP1 protein (His tag N-Terminus)

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Recombinant Human SSBP1 protein (His tag N-Terminus) is a Human Full Length protein, in the 17 to 148 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

SSBP, SSBP1, Mt-SSB, MtSSB, PWP1-interacting protein 17

1 Images
SDS-PAGE - Recombinant Human SSBP1 protein (His tag N-Terminus) (AB140730)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SSBP1 protein (His tag N-Terminus) (AB140730)

15% SDS-PAGE analysis of ab140730 (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q04837

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSESETTTSLVLERSLNRVHLLGRVGQDPVLRQVEGKNPVTIFSLATNEMWRSGDSEVYQLGDVSQKTTWHRISVFRPGLRDVAYQYVKKGSRIYLEGKIDYGEYMDKNNVRRQATTIIADNIIFLSDQTKEKE","proteinLength":"Full Length","predictedMolecularWeight":"17 kDa","actualMolecularWeight":null,"aminoAcidEnd":148,"aminoAcidStart":17,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q04837","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SSBP1 also known as Single-Stranded DNA Binding Protein 1 is a mitochondrial protein with an approximate mass of 14 kDa. SSBP1 binds to single-stranded DNA and stabilizes it during DNA replication and repair processes. It functions by preventing the DNA from re-annealing or forming secondary structures which helps in maintaining genomic stability. This protein is mostly expressed in tissues with high energy demands such as muscle and liver reflecting its significant role in mitochondrial activities.
Biological function summary

Single-Stranded DNA Binding Protein 1 participates in essential mitochondrial DNA maintenance processes and interacts with other proteins to form functional complexes. It associates with mitochondrial DNA polymerase gamma and other replication machinery components to ensure accurate DNA replication. These interactions are significant for proper mitochondrial function since they prevent mutations that could compromise cellular energy production.

Pathways

SSBP1 engages actively in the mitochondrial DNA replication and repair pathway. It works closely with proteins like TWINKLE helicase and DNA polymerase gamma complex facilitating efficient replication and repair of mitochondrial DNA. These pathways are pivotal for mitochondrial biogenesis and function impacting cellular energy metabolism and overall mitochondrial health.

Alterations in SSBP1 have links to mitochondrial dysfunctions including disorders like mitochondrial myopathy and progressive external ophthalmoplegia. Dysfunctional SSBP1 may lead to impaired DNA replication or repair in mitochondria contributing to these conditions. Additionally it relates to TWINKLE helicase mutations which also disrupt mitochondrial DNA maintenance further emphasizing the importance of SSBP1 in maintaining cellular energy balance.
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Specifications

Form

Liquid

Additional notes

ab140730 was purified using conventional chromatography techniques.

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General info

Function

Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA) (PubMed : 21953457, PubMed : 23290262, PubMed : 31550240). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork (PubMed : 12975372, PubMed : 15167897, PubMed : 21953457, PubMed : 26446790, PubMed : 31550240). Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity (PubMed : 21953457, PubMed : 26446790, PubMed : 31550240). In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK (PubMed : 12975372). May also function in mtDNA repair (PubMed : 23290262).

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA) (PubMed : 21953457, PubMed : 23290262, PubMed : 31550240). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork (PubMed : 12975372, PubMed : 15167897, PubMed : 21953457, PubMed : 26446790, PubMed : 31550240). Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity (PubMed : 21953457, PubMed : 26446790, PubMed : 31550240). In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK (PubMed : 12975372). May also function in mtDNA repair (PubMed : 23290262).
See full target information SSBP1
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Complementary Products

Primary Antibodies

AB224053

Anti-SSBP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB224053)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB228576

Anti-POLRMT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLRMT antibody (AB228576)

  • 0
  • 0
Primary Antibodies

AB185352

Anti-DUBA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUBA2 antibody (AB185352)

  • 0
  • 0

Product promise

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