Recombinant Human ST14 protein is a Human Full Length protein, in the 1 to 855 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M G S D R A R K G G G G P K D F G A G L K Y N S R H E K V N G L E E G V E F L P V N N V K K V E K H G P G R W V V L A A V L I G L L L V L L G I G F L V W H L Q Y R D V R V Q K V F N G Y M R I T N E N F V D A Y E N S N S T E F V S L A S K V K D A L K L L Y S G V P F L G P Y H K E S A V T A F S E G S V I A Y Y W S E F S I P Q H L V E E A E R V M A E E R V V M L P P R A R S L K S F V V T S V V A F P T D S K T V Q R T Q D N S C S F G L H A R G V E L M R F T T P G F P D S P Y P A H A R C Q W A L R G D A D S V L S L T F R S F D L A S C D E R G S D L V T V Y N T L S P M E P H A L V Q L C G T Y P P S Y N L T F H S S Q N V L L I T L I T N T E R R H P G F E A T F F Q L P R M S S C G G R L R K A Q G T F N S P Y Y P G H Y P P N I D C T W N I E V P N N Q H V K V R F K F F Y L L E P G V P A G T C P K D Y V E I N G E K Y C G E R S Q F V V T S N S N K I T V R F H S D Q S Y T D T G F L A E Y L S Y D S S D P C P G Q F T C R T G R C I R K E L R C D G W A D C T D H S D E L N C S C D A G H Q F T C K N K F C K P L F W V C D S V N D C G D N S D E Q G C S C P A Q T F R C S N G K C L S K S Q Q C N G K D D C G D G S D E A S C P K V N V V T C T K H T Y R C L N G L C L S K G N P E C D G K E D C S D G S D E K D C D C G L R S F T R Q A R V V G G T D A D E G E W P W Q V S L H A L G Q G H I C G A S L I S P N W L V S A A H C Y I D D R G F R Y S D P T Q W T A F L G L H D Q S Q R S A P G V Q E R R L K R I I S H P F F N D F T F D Y D I A L L E L E K P A E Y S S M V R P I C L P D A S H V F P A G K A I W V T G W G H T Q Y G G T G A L I L Q K G E I R V I N Q T T C E N L L P Q Q I T P R M M C V G F L S G G V D S C Q G D S G G P L S S V E A D G R I F Q A G V V S W G D G C A Q R N K P G V Y T R L P L F R D W I K E N T G V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site (PubMed:10373424). Involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing (PubMed:18843291). Proteolytically cleaves and therefore activates TMPRSS13 (PubMed:28710277).
Alternative names
PRSS14, SNC19, TADG15, ST14, Suppressor of tumorigenicity 14 protein, Matriptase, Membrane-type serine protease 1, Prostamin, Serine protease 14, Serine protease TADG-15, Tumor-associated differentially-expressed gene 15 protein, MT-SP1
Recommended products
Recombinant Human ST14 protein is a Human Full Length protein, in the 1 to 855 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q9Y5Y6-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M G S D R A R K G G G G P K D F G A G L K Y N S R H E K V N G L E E G V E F L P V N N V K K V E K H G P G R W V V L A A V L I G L L L V L L G I G F L V W H L Q Y R D V R V Q K V F N G Y M R I T N E N F V D A Y E N S N S T E F V S L A S K V K D A L K L L Y S G V P F L G P Y H K E S A V T A F S E G S V I A Y Y W S E F S I P Q H L V E E A E R V M A E E R V V M L P P R A R S L K S F V V T S V V A F P T D S K T V Q R T Q D N S C S F G L H A R G V E L M R F T T P G F P D S P Y P A H A R C Q W A L R G D A D S V L S L T F R S F D L A S C D E R G S D L V T V Y N T L S P M E P H A L V Q L C G T Y P P S Y N L T F H S S Q N V L L I T L I T N T E R R H P G F E A T F F Q L P R M S S C G G R L R K A Q G T F N S P Y Y P G H Y P P N I D C T W N I E V P N N Q H V K V R F K F F Y L L E P G V P A G T C P K D Y V E I N G E K Y C G E R S Q F V V T S N S N K I T V R F H S D Q S Y T D T G F L A E Y L S Y D S S D P C P G Q F T C R T G R C I R K E L R C D G W A D C T D H S D E L N C S C D A G H Q F T C K N K F C K P L F W V C D S V N D C G D N S D E Q G C S C P A Q T F R C S N G K C L S K S Q Q C N G K D D C G D G S D E A S C P K V N V V T C T K H T Y R C L N G L C L S K G N P E C D G K E D C S D G S D E K D C D C G L R S F T R Q A R V V G G T D A D E G E W P W Q V S L H A L G Q G H I C G A S L I S P N W L V S A A H C Y I D D R G F R Y S D P T Q W T A F L G L H D Q S Q R S A P G V Q E R R L K R I I S H P F F N D F T F D Y D I A L L E L E K P A E Y S S M V R P I C L P D A S H V F P A G K A I W V T G W G H T Q Y G G T G A L I L Q K G E I R V I N Q T T C E N L L P Q Q I T P R M M C V G F L S G G V D S C Q G D S G G P L S S V E A D G R I F Q A G V V S W G D G C A Q R N K P G V Y T R L P L F R D W I K E N T G V
- Accession
- Q9Y5Y6
- Protein length
- Full Length
- Predicted molecular weight
- 120.12 kDa
- Amino acids
- 1 to 855
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site (PubMed:10373424). Involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing (PubMed:18843291). Proteolytically cleaves and therefore activates TMPRSS13 (PubMed:28710277).
- Sequence similarities
Belongs to the peptidase S1 family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ST14 also known as matriptase is a serine protease with a molecular mass of about 92 kDa. It exists as a single-chain zymogen and activates through cleavage to form a two-chain form. Matriptase resides on the surface of epithelial cells and shows high expression in several tissues such as the epidermis prostate and gastrointestinal tract. This protease plays a part in tissue homeostasis by initiating proteolytic cascades that modify the extracellular matrix and influence cellular processes.
- Biological function summary
Matriptase regulates proteolytic activation events in the extracellular environment. It instigates cascades that activate specific growth factors and proteases playing a role in cell proliferation and migration. Matriptase forms a complex with hepatocyte growth factor activator inhibitor-1 (HAI-1) which modulates its activity. The balance between matriptase and its inhibitors is important for controlling epithelial cell dynamics and maintaining a healthy cellular environment.
- Pathways
Matriptase contributes to key signaling pathways like the epidermal growth factor receptor (EGFR) pathway and the plasminogen activation pathway. Within the EGFR signaling matriptase activates growth factors that influence cell proliferation and survival. It also intersects with proteins like epithelial growth factor receptor and plasminogen which are significant for cellular functions and homeostasis. These pathways are important for maintaining normal tissue function and responding to cellular stress.
- Associated diseases and disorders
Deregulation of matriptase is linked to conditions like cancer and ichthyosis. Matriptase overexpression or aberrant activity often relates to tumor progression and cancer metastasis particularly in breast and prostate cancer. Dysregulation also associates with the skin disorder ichthyosis due to imbalance with its inhibitor HAI-1 leading to defective epidermal permeability. Understanding matriptase's role in these diseases helps in designing therapeutic strategies and targeting the protease with specific inhibitors.
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1 product image
SDS-PAGE - Recombinant Human ST14 protein (ab114597)
ab114597 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.
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