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AB233601

Recombinant human ST2 protein (Active) (His tag C-Terminus)

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(1 Publication)

Recombinant human ST2 protein (Active) (His tag C-Terminus) is a Human Fragment protein, in the 19 to 328 aa range, expressed in Baculovirus infected insect cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

DER4, ST2, T1, IL1RL1, Interleukin-1 receptor-like 1, Protein ST2

1 Images
SDS-PAGE - Recombinant human ST2 protein (Active) (His tag C-Terminus) (AB233601)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human ST2 protein (Active) (His tag C-Terminus) (AB233601)

15% SDS-PAGE analysis of 3 μg ab233601.

MW : 40-57 kDa (SDS-PAGE under reducing conditions).

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 10 ng/ml with IL-33.

Accession

Q01638

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"KFSKQSWGLENEALIVRCPRQGKPSYTVDWYYSQTNKSIPTQERNRVFASGQLLKFLPAAVADSGIYTCIVRSPTFNRTGYANVTIYKKQSDCNVPDYLMYSTVSGSEKNSKIYCPTIDLYNWTAPLEWFKNCQALQGSRYRAHKSFLVIDNVMTEDAGDYTCKFIHNENGANYSVTATRSFTVKDEQGFSLFPVIGAPAQNEIKEVEIGKNANLTCSACFGKGTQFLAAVLWQLNGTKITDFGEPRIQQEEGQNQSFSNGLACLDMVLRIADVKEEDLLLQYDCLALNLHGLRRHTVRLSRKNPIDHHSLEHHHHHH","proteinLength":"Fragment","predictedMolecularWeight":"36 kDa","actualMolecularWeight":null,"aminoAcidEnd":328,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":"Q01638","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.
Biological function summary

ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.

Pathways

IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.

ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.
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Specifications

Form

Liquid

Additional notes

Affinity purified

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General info

Function

Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed : 35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed : 16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. Isoform B. Inhibits IL-33 signaling.

Sequence similarities

Belongs to the interleukin-1 receptor family.

Post-translational modifications

Ubiquitinated at Lys-321 in a FBXL19-mediated manner; leading to proteasomal degradation. Ubiquitination by TRAF6 via 'Lys-27'-linked polyubiquitination and deubiquitination by USP38 serves as a critical regulatory mechanism for fine-tuning IL1RL1-mediated inflammatory response (PubMed:35238669).

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Product protocols

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Target data

Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed : 35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed : 16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. Isoform B. Inhibits IL-33 signaling.
See full target information IL1RL1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 15:10539-10546 PubMed33408473

2020

Development of a Novel Homogeneous Nanoparticle-Based Assay for Rapid and High-Throughput Quantitation of the sST2 Protein in Human Serum.

Applications

Unspecified application

Species

Unspecified reactive species

Shenxia Gao,Junpu Li
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