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Recombinant human ST2 protein (Active) is a Human Fragment protein, in the 19 to 328 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.

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Images

SDS-PAGE - Recombinant human ST2 protein (Active) (AB233601), expandable thumbnail

Publications

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

K F S K Q S W G L E N E A L I V R C P R Q G K P S Y T V D W Y Y S Q T N K S I P T Q E R N R V F A S G Q L L K F L P A A V A D S G I Y T C I V R S P T F N R T G Y A N V T I Y K K Q S D C N V P D Y L M Y S T V S G S E K N S K I Y C P T I D L Y N W T A P L E W F K N C Q A L Q G S R Y R A H K S F L V I D N V M T E D A G D Y T C K F I H N E N G A N Y S V T A T R S F T V K D E Q G F S L F P V I G A P A Q N E I K E V E I G K N A N L T C S A C F G K G T Q F L A A V L W Q L N G T K I T D F G E P R I Q Q E E G Q N Q S F S N G L A C L D M V L R I A D V K E E D L L L Q Y D C L A L N L H G L R R H T V R L S R K N P I D H H S L E H H H H H H

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed:35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed:16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).Isoform BInhibits IL-33 signaling.

Alternative names

Recommended products

Recombinant human ST2 protein (Active) is a Human Fragment protein, in the 19 to 328 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Applications

SDS-PAGE, FuncS

Biological activity

Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 10 ng/ml with IL-33.

Accession
Q01638-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)

Sequence info

Amino acid sequence

K F S K Q S W G L E N E A L I V R C P R Q G K P S Y T V D W Y Y S Q T N K S I P T Q E R N R V F A S G Q L L K F L P A A V A D S G I Y T C I V R S P T F N R T G Y A N V T I Y K K Q S D C N V P D Y L M Y S T V S G S E K N S K I Y C P T I D L Y N W T A P L E W F K N C Q A L Q G S R Y R A H K S F L V I D N V M T E D A G D Y T C K F I H N E N G A N Y S V T A T R S F T V K D E Q G F S L F P V I G A P A Q N E I K E V E I G K N A N L T C S A C F G K G T Q F L A A V L W Q L N G T K I T D F G E P R I Q Q E E G Q N Q S F S N G L A C L D M V L R I A D V K E E D L L L Q Y D C L A L N L H G L R R H T V R L S R K N P I D H H S L E H H H H H H

Accession

Q01638

Protein length

Fragment

Predicted molecular weight

36 kDa

Amino acids

19 to 328

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Liquid

Additional notes

Affinity purified

General info

Function

Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed:35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed:16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).

Sequence similarities

Belongs to the interleukin-1 receptor family.

Post-translational modifications

Ubiquitinated at Lys-321 in a FBXL19-mediated manner; leading to proteasomal degradation. Ubiquitination by TRAF6 via 'Lys-27'-linked polyubiquitination and deubiquitination by USP38 serves as a critical regulatory mechanism for fine-tuning IL1RL1-mediated inflammatory response (PubMed:35238669).

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.

Biological function summary

ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.

Pathways

IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.

Associated diseases and disorders

ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.

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1 product image

  • SDS-PAGE - Recombinant human ST2 protein (Active) (ab233601), expandable thumbnail

    SDS-PAGE - Recombinant human ST2 protein (Active) (ab233601)

    15% SDS-PAGE analysis of 3 μg ab233601.

    MW: 40-57 kDa (SDS-PAGE under reducing conditions).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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