Recombinant Human ST2 protein (Active) is a Human Fragment protein, in the 19 to 328 aa range, expressed in HEK 293, with >=95% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
>=95% SDS-PAGE
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
Yes
Amino acid sequence
K F S K Q S W G L E N E A L I V R C P R Q G K P S Y T V D W Y Y S Q T N K S I P T Q E R N R V F A S G Q L L K F L P A A V A D S G I Y T C I V R S P T F N R T G Y A N V T I Y K K Q S D C N V P D Y L M Y S T V S G S E K N S K I Y C P T I D L Y N W T A P L E W F K N C Q A L Q G S R Y R A H K S F L V I D N V M T E D A G D Y T C K F I H N E N G A N Y S V T A T R S F T V K D E Q G F S L F P V I G A P A Q N E I K E V E I G K N A N L T C S A C F G K G T Q F L A A V L W Q L N G T K I T D F G E P R I Q Q E E G Q N Q S F S N G L A C L D M V L R I A D V K E E D L L L Q Y D C L A L N L H G L R R H T V R L S R K N P I D H H S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for interleukin-33 (IL-33); signaling requires association of the coreceptor IL1RAP. Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.Isoform BInhibits IL-33 signaling.
Alternative names
Interleukin-1 receptor-like 1, Protein ST2, T1, ST2, IL1RL1, DER4
Recommended products
Recombinant Human ST2 protein (Active) is a Human Fragment protein, in the 19 to 328 aa range, expressed in HEK 293, with >=95% purity and suitable for SDS-PAGE.
Alternative names
Interleukin-1 receptor-like 1, Protein ST2, T1, ST2, IL1RL1, DER4
Key facts
- Purity
>=95% SDS-PAGE
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
Yes
- Biological activity
Fully biologically active determined by its ability to inhibit IL-33 dependent proliferation of D10S cells. The ED50 ≤ 0.27 μg/ml, corresponding to a specific activity of 3.7 x 103 units/mg.
- Accession
- Q01638-1
- Animal free
Yes
- Species
Human
- Reconstitution
Store lyophilized form at room temperature. Reconstitute in PBS, aliquot and store at -80 °C for 12 months or +4 °C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
K F S K Q S W G L E N E A L I V R C P R Q G K P S Y T V D W Y Y S Q T N K S I P T Q E R N R V F A S G Q L L K F L P A A V A D S G I Y T C I V R S P T F N R T G Y A N V T I Y K K Q S D C N V P D Y L M Y S T V S G S E K N S K I Y C P T I D L Y N W T A P L E W F K N C Q A L Q G S R Y R A H K S F L V I D N V M T E D A G D Y T C K F I H N E N G A N Y S V T A T R S F T V K D E Q G F S L F P V I G A P A Q N E I K E V E I G K N A N L T C S A C F G K G T Q F L A A V L W Q L N G T K I T D F G E P R I Q Q E E G Q N Q S F S N G L A C L D M V L R I A D V K E E D L L L Q Y D C L A L N L H G L R R H T V R L S R K N P I D H H S
- Accession
- Protein length
Fragment
- Predicted molecular weight
35 kDa
- Amino acids
19 to 328
- Nature
Recombinant
Specifications
- Form
Lyophilized
General info
- Function
Receptor for interleukin-33 (IL-33); signaling requires association of the coreceptor IL1RAP. Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.
- Sequence similarities
Belongs to the interleukin-1 receptor family.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- Activity summary
ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.
- Biological function summary
ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.
- Pathways
IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.
- Associated diseases and disorders
ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.
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2 product images
SDS-PAGE - Recombinant Human ST2 protein (Active) (ab283466)
SDS-page analysis of ab283466
Biological Activity - Recombinant Human ST2 protein (Active) (ab283466)
Fully biologically active determined by its ability to inhibit IL-33 dependent proliferation of D10S cells. The ED50 ≤ 0.27 µg/ml, corresponding to a specific activity of 3.7 x 10^3 units/mg.
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