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AB176053

Recombinant Human ST3GAL5 protein (denatured) (His tag N-Terminus)

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Recombinant Human ST3GAL5 protein (denatured) (His tag N-Terminus) is a Human Fragment protein, in the 83 to 418 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

SIAT9, UNQ2510/PRO5998, ST3GAL5, GM3 synthase, Ganglioside GM3 synthase, ST3Gal V, Sialyltransferase 9, ST3GalV

1 Images
SDS-PAGE - Recombinant Human ST3GAL5 protein (denatured) (His tag N-Terminus) (AB176053)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ST3GAL5 protein (denatured) (His tag N-Terminus) (AB176053)

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9UNP4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris-HCl buffer

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSLKLNYTTEECDMKKMHYVDPDHVKRAQKYAQQVLQKECRPKFAKTSMALLFEHRYSVDLLPFVQKAPKDSEAESKYDPPFGFRKFSSKVQTLLELLPEHDLPEHLKAKTCRRCVVIGSGGILHGLELGHTLNQFDVVIRLNSAPVEGYSEHVGNKTTIRMTYPEGAPLSDLEYYSNDLFVAVLFKSVDFNWLQAMVKKETLPFWVRLFFWKQVAEKIPLQPKHFRILNPVIIKETAFDILQYSEPQSRFWGRDKNVPTIGVIAVVLATHLCDEVSLAGFGYDLNQPRTPLHYFDSQCMAAMNFQTMHNVTTETKFLLKLVKEGVVKDLSGGIDREF","proteinLength":"Fragment","predictedMolecularWeight":"41 kDa","actualMolecularWeight":null,"aminoAcidEnd":418,"aminoAcidStart":83,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UNP4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST3GAL5 also known as GM3 synthase or SAT-I is an enzyme that catalyzes the transfer of sialic acid to lactosylceramide forming ganglioside GM3. This enzyme belongs to the glycosyltransferase family and has a molecular mass of approximately 43 kDa. It is expressed in a variety of tissues with significant presence in the brain where it plays role in neural cell interactions.
Biological function summary

The enzyme ST3GAL5 influences cell communication proliferation and differentiation through the synthesis of gangliosides. It does not function as part of a larger complex but acts independently to produce ganglioside GM3. GM3 is significant in membrane microdomains known as lipid rafts which are involved in cellular signaling. Through influencing these lipid rafts ST3GAL5 indirectly affects numerous cellular processes including signaling events in nerve cells.

Pathways

ST3GAL5 is integral in the lipid metabolism pathway particularly in ganglioside biosynthesis. It interacts with other enzymes involved in the production of more complex gangliosides such as ST8SIA1 which further converts GM3 to GD3. Additionally it plays role in the insulin signaling pathway where GM3 participates in modulating insulin receptor function. ST3GAL5's enzymatic product GM3 can affect receptor activity by insertion into lipid rafts influencing insulin signaling efficiency.

ST3GAL5 links to disorders like hereditary spastic paraplegia and epilepsy. Mutations in the ST3GAL5 gene can lead to disruptions in ganglioside metabolism resulting in impaired neural function. Moreover its dysfunctional biosynthesis affects the vascular endothelial growth factor (VEGF) signaling pathway contributing to irregularities in angiogenesis that may relate to diseases like cancer. The intricate balance maintained by ST3GAL5 and associated proteins in these pathways highlights its importance in maintaining normal cellular function.
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Specifications

Form

Liquid

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General info

Function

Transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose (Gal) of glycosphingolipids forming gangliosides (important molecules involved in the regulation of multiple cellular processes, including cell proliferation and differentiation, apoptosis, embryogenesis, development, and oncogenesis) (PubMed : 16934889, PubMed : 9822625). Mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1), although less preferentially than beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer (LacCer) (PubMed : 16934889).

Sequence similarities

Belongs to the glycosyltransferase 29 family.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

Transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose (Gal) of glycosphingolipids forming gangliosides (important molecules involved in the regulation of multiple cellular processes, including cell proliferation and differentiation, apoptosis, embryogenesis, development, and oncogenesis) (PubMed : 16934889, PubMed : 9822625). Mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1), although less preferentially than beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer (LacCer) (PubMed : 16934889).
See full target information ST3GAL5
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