Recombinant Human STAT4 protein
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Recombinant Human STAT4 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB.
View Alternative Names
Signal transducer and activator of transcription 4, STAT4
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human STAT4 protein (AB84634)
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
STAT4 is integral to the differentiation of naïve T cells into T helper 1 (Th1) cells. These Th1 cells play a central role in orchestrating immune responses against intracellular pathogens. STAT4 activation requires two distinct steps involving JAK kinases and subsequent binding to DNA regulatory elements of target genes. STAT4 does not function as part of a large protein complex but rather operates as a dimer following phosphorylation.
Pathways
STAT4 participates strongly in the JAK-STAT signaling pathway fundamental for immune cell maturation and response. The JAK-STAT pathway regulates several biological processes including proliferation and inflammation. STAT4 works closely with STAT1 in promoting Th1 differentiation while also interacting with other proteins like IL-12 to modulate immune activity. These interactions emphasize its influence in immune responses and could affect inflammatory pathways.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response (PubMed : 10961885, PubMed : 37256972, PubMed : 8943379). Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma (PubMed : 12213961, PubMed : 35614130). Participates also in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps (By similarity). After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated (PubMed : 10415122, PubMed : 7638186). Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes. Although IL12 appears to be the predominant activating signal, STAT4 can also be phosphorylated and activated in response to IFN-gamma stimulation via JAK1 and TYK2 and in response to different interleukins including IL23, IL2 and IL35 (PubMed : 11114383, PubMed : 34508746). Transcription activation of IFN-gamma gene is mediated by interaction with JUN that forms a complex that efficiently interacts with the AP-1-related sequence of the IFN-gamma promoter (By similarity). In response to IFN-alpha/beta signaling, acts as a transcriptional repressor and suppresses IL5 and IL13 mRNA expression during response to T-cell receptor (TCR) activation (PubMed : 26990433).
Sequence similarities
Belongs to the transcription factor STAT family.
Post-translational modifications
Acetylation at Lys-667 is required for JAK2-mediated phosphorylation and activation of STAT4.. Tyrosine phosphorylated upon IL12 and IFN-alpha activation, but not by IFN-gamma in T-lymphocytes and NK cells (PubMed:8943379). Serine phosphorylation is required for maximal transcriptional activity but not for DNA binding (PubMed:8943379). Phosphorylation by MAP2K6 at Ser-721 is required for full transcriptional activity induced by IL12 (PubMed:10961885). However this serine phosphorylation is not required for cell proliferation although critical for IFN-gamma production (PubMed:12213961).
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
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