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AB84627

Recombinant Human STAT5a protein

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(1 Publication)

Recombinant Human STAT5a protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB.

View Alternative Names

STAT5, STAT5A, Signal transducer and activator of transcription 5A

2 Images
Western blot - Recombinant Human STAT5a protein (AB84627)
  • WB

Unknown

Western blot - Recombinant Human STAT5a protein (AB84627)

All lanes:

Western blot - Anti-STAT5a antibody [E289] (<a href='/en-us/products/primary-antibodies/stat5a-antibody-e289-ab32043'>ab32043</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant Human STAT5a protein (ab84627) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 90 kDa

true

Exposure time: 20s

SDS-PAGE - Recombinant Human STAT5a protein (AB84627)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human STAT5a protein (AB84627)

SDS Page analysis of ab84627

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P42229

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p><a href='/en-us/products/primary-antibodies/stat5a-antibody-e289-ab32043'>ab32043</a> can be used as a WB positive control in conjunction with ab84627.</p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P42229","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAT5a also known as Signal Transducer and Activator of Transcription 5A acts as a transcription factor in the cell. STAT5a weighs approximately 90 kDa and expresses in various tissues including the liver muscle and hematopoietic cells. It plays a central role in mediating cellular responses to cytokines and growth factors particularly those in the interleukin-2 (IL-2) family and growth hormone (GH).
Biological function summary

Many systems use STAT5a to regulate cell proliferation differentiation and survival. It often forms a complex with other proteins like STAT5b which is a closely related counterpart and both contribute to similar cellular processes. STAT5a can dimerize upon activation allowing it to move to the cell nucleus to influence gene expression directly.

Pathways

Researchers place STAT5a prominently in the JAK-STAT signaling pathway. This pathway is essential for transmitting information received from extracellular chemical signals to the cell nucleus resulting in the activation of genes controlling cellular functions. STAT5a works together with Janus kinases (JAKs) to mediate various biological responses including hormone signaling and immune function. It also interacts with proteins like IL-2 receptor subunits which are involved in many cytokine-driven processes.

Disturbances in STAT5a activity link specifically with conditions like leukemia and breast cancer. Overactivation or mutation can lead to uncontrolled cell growth and survival contributing to these diseases. In leukemia STAT5a frequently partners with other proteins like BCR-ABL which is known for its role in chronic myeloid leukemia influencing oncogenic pathways. In breast cancer alterations in STAT5a signaling may interact with estrogen and progesterone receptors affecting tumor progression and response to therapies.
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Specifications

Form

Liquid

Additional notes

Purity >90% as determined by densitometry. Affinity purified.

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General info

Function

Carries out a dual function : signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

Sequence similarities

Belongs to the transcription factor STAT family.

Post-translational modifications

Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO (By similarity). Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus (PubMed:21135090). Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity (By similarity). Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3 (PubMed:14504097). Alternatively, can be phosphorylated by JAK2 at Tyr-694 (PubMed:12529425).. ISGylated.

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Product protocols

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Target data

Carries out a dual function : signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
See full target information STAT5A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Carcinogenesis 35:2244-53 PubMed24925029

2014

Inhibition of STAT5a by Naa10p contributes to decreased breast cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zeng,Li Min,Yong Han,Lin Meng,Caiyun Liu,Yuntao Xie,Bin Dong,Lixin Wang,Beihai Jiang,Huiyu Xu,Qing Zhuang,Chuanke Zhao,Like Qu,Chengchao Shou
View all publications
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