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AB177658

Recombinant Human STEAP4 protein (denatured)

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Recombinant Human STEAP4 protein (denatured) is a Human Fragment protein, in the 1 to 152 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

STAMP2, TNFAIP9, STEAP4, Metalloreductase STEAP4, Six-transmembrane epithelial antigen of prostate 4, SixTransMembrane protein of prostate 2

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SDS-PAGE - Recombinant Human STEAP4 protein (denatured) (AB177658)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human STEAP4 protein (denatured) (AB177658)

SDS-PAGE of ab177658 (3µg) under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DDDDK tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q687X5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMEKTCIDALPLTMNSSEKQETVCIFGTGDFGRSLGLKMLQCGYSVVFGSRNPQKTTLLPSGAEVLSYSEAAKKSGIIIIAIHREHYDFLTELTEVLNGKILVDISNNLKINQYPESNAEYLAHLVPGAHVVKAFNTISAWALQSGALDASRQ","proteinLength":"Fragment","predictedMolecularWeight":"20.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":152,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q687X5","tags":[{"tag":"His-DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STEAP4 also known as STEAP4 metalloreductase or TIARP is a membrane-associated protein with a molecular mass of approximately 51 kDa. It mainly functions as a metalloreductase playing a role in the reduction of metal ions particularly iron and copper within cells. You find its expression in various tissues with higher levels in the adipose tissue liver and prostate. This protein is integral to cellular processes that require metal ion homeostasis influencing several pathways through its enzymatic activity on metal reduction.
Biological function summary

The function of STEAP4 extends beyond mere metal ion reduction. It contributes to cellular metabolism and inflammatory response regulation. Researchers have noted its involvement in the modulation of reactive oxygen species (ROS) which are important for detoxification and signaling. This protein does not function in isolation but may work in concert with other proteins in complexes related to the cell membrane and energy metabolism.

Pathways

STEAP4 participates in critical metabolic and inflammatory pathways. In particular it plays a role in the JNK and NF-kB signaling pathways which impact cellular stress responses and inflammation. Interactions with additional regulatory proteins such as TNF-alpha and IL-6 further integrate STEAP4 into these pathways linking its functions with larger cellular processes that respond to external stimuli and stress factors.

STEAP4 has important connections to metabolic and inflammatory conditions. Research shows its involvement in obesity-related inflammation and insulin resistance linking it with metabolic syndrome conditions. You also find associations between STEAP4 expression and type 2 diabetes as its dysfunction may impact glucose and lipid metabolism. This protein's interactions with TNF-alpha and IL-6 also suggest a strong link to chronic inflammatory states which can exacerbate metabolic disorders.
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Specifications

Form

Liquid

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General info

Function

Integral membrane protein that functions as a NADPH-dependent ferric-chelate reductase, using NADPH from one side of the membrane to reduce a Fe(3+) chelate that is bound on the other side of the membrane. Mediates sequential transmembrane electron transfer from NADPH to FAD and onto heme, and finally to the Fe(3+) chelate (PubMed : 30337524). Can also reduce Cu(2+) to Cu(1+) (By similarity). Plays a role in systemic metabolic homeostasis, integrating inflammatory and metabolic responses (By similarity). Associated with obesity and insulin-resistance (PubMed : 18381574, PubMed : 18430367). Involved in inflammatory arthritis, through the regulation of inflammatory cytokines (PubMed : 19660107). Inhibits anchorage-independent cell proliferation (PubMed : 19787193).

Sequence similarities

Belongs to the STEAP family.

Subcellular localisation

Early endosome membrane

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Product protocols

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Target data

Integral membrane protein that functions as a NADPH-dependent ferric-chelate reductase, using NADPH from one side of the membrane to reduce a Fe(3+) chelate that is bound on the other side of the membrane. Mediates sequential transmembrane electron transfer from NADPH to FAD and onto heme, and finally to the Fe(3+) chelate (PubMed : 30337524). Can also reduce Cu(2+) to Cu(1+) (By similarity). Plays a role in systemic metabolic homeostasis, integrating inflammatory and metabolic responses (By similarity). Associated with obesity and insulin-resistance (PubMed : 18381574, PubMed : 18430367). Involved in inflammatory arthritis, through the regulation of inflammatory cytokines (PubMed : 19660107). Inhibits anchorage-independent cell proliferation (PubMed : 19787193).
See full target information STEAP4
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