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AB134623

Recombinant Human STELLAR protein (denatured) (His tag N-Terminus)

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Recombinant Human STELLAR protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 159 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

STELLAR, DPPA3, Developmental pluripotency-associated protein 3, Stella-related protein

1 Images
SDS-PAGE - Recombinant Human STELLAR protein (denatured) (His tag N-Terminus) (AB134623)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human STELLAR protein (denatured) (His tag N-Terminus) (AB134623)

15% SDS-PAGE analysis of 3 µg ab134623.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q6W0C5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Stella
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMDPSQFNPTYIPGSPQMLTEENSRDDSGASQISSETLIKNLSNLTINASSESVSPLSEALLRRESVGAAVLREIEDEWLYSRRGVRTLLSVQREKMARLRYMLLGGVRTHERRPTNKEPKGVKKESRPFKCPCSFCVSNGWDPSENARIGNQDTKPLQP","proteinLength":"Full Length","predictedMolecularWeight":"20.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":159,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q6W0C5","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STELLAR also known as 'STELLA' or 'DPPA2' acts mechanically as a regulator of pluripotency and early embryonic development. It weighs approximately 17 kDa. This protein expresses itself in pluripotent stem cells and germ cells. Researchers have found subcellular localization of STELLAR mainly in the nucleus suggesting critical functions in gene regulation during cell differentiation.
Biological function summary

The protein plays an important role in maintaining cell totipotency and germ cell specification. STELLAR interacts with several cellular complexes that are pivotal during embryogenesis. By regulating specific genes STELLAR contributes to the repression of inappropriate gene expression in pluripotent cells impacting cellular fate outcomes during development.

Pathways

STELLAR participates in the pluripotency regulatory network involving significant pathways like the Wnt signaling pathway. This protein's regulatory mechanisms align with other transcription factors such as NANOG and OCT4 highlighting its role in the maintenance of the pluripotent state. The interplay among these factors establishes a complex circuit essential for the retention of stem cell properties.

Aberrant expression or regulation of STELLAR associates with certain cancers. Its connection to disorders like germ cell tumors suggests an oncogenic potential when dysregulated. Moreover STELLAR's interaction with proteins such as LIN28 highlights its involvement in tumorigenic processes shedding light on its significance in both normal development and pathological states.
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Specifications

Form

Liquid

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General info

Function

Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization (PubMed : 35314832). In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process (By similarity). Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC : specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation (By similarity). Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark (By similarity). Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis (By similarity). May be important for the totipotent/pluripotent states continuing through preimplantation development (By similarity). Also involved in chromatin condensation in oocytogenesis (By similarity).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization (PubMed : 35314832). In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process (By similarity). Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC : specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation (By similarity). Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark (By similarity). Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis (By similarity). May be important for the totipotent/pluripotent states continuing through preimplantation development (By similarity). Also involved in chromatin condensation in oocytogenesis (By similarity).
See full target information DPPA3
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