Recombinant Human STEP / PTPN5 protein is a Human Full Length protein, in the 1 to 565 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M N Y E G A R S E R E N H A A D D S E G G A L D M C C S E R L P G L P Q P I V M E A L D E A E G L Q D S Q R E M P P P P P P S P P S D P A Q K P P P R G A G S H S L T V R S S L C L F A A S Q F L L A C G V L W F S G Y G H I W S Q N A T N L V S S L L T L L K Q L E P T A W L D S G T W G V P S L L L V F L S V G L V L V T T L V W H L L R T P P E P P T P L P P E D R R Q S V S R Q P S F T Y S E W M E E K I E D D F L D L D P V P E T P V F D C V M D I K P E A D P T S L T V K S M G L Q E R R G S N V S L T L D M C T P G C N E E G F G Y L M S P R E E S A R E Y L L S A S R V L Q A E E L H E K A L D P F L L Q A E F F E I P M N F V D P K E Y D I P G L V R K N R Y K T I L P N P H S R V C L T S P D P D D P L S S Y I N A N Y I R G Y G G E E K V Y I A T Q G P I V S T V A D F W R M V W Q E H T P I I V M I T N I E E M N E K C T E Y W P E E Q V A Y D G V E I T V Q K V I H T E D Y R L R L I S L K S G T E E R G L K H Y W F T S W P D Q K T P D R A P P L L H L V R E V E E A A Q Q E G P H C A P I I V H C S A G I G R T G C F I A T S I C C Q Q L R Q E G V V D I L K T T C Q L R Q D R G G M I Q T C E Q Y Q F V H H V M S L Y E K Q L S H Q S P E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
Alternative names
Tyrosine-protein phosphatase non-receptor type 5, Neural-specific protein-tyrosine phosphatase, Striatum-enriched protein-tyrosine phosphatase, STEP, PTPN5
Recommended products
Recombinant Human STEP / PTPN5 protein is a Human Full Length protein, in the 1 to 565 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P54829-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M N Y E G A R S E R E N H A A D D S E G G A L D M C C S E R L P G L P Q P I V M E A L D E A E G L Q D S Q R E M P P P P P P S P P S D P A Q K P P P R G A G S H S L T V R S S L C L F A A S Q F L L A C G V L W F S G Y G H I W S Q N A T N L V S S L L T L L K Q L E P T A W L D S G T W G V P S L L L V F L S V G L V L V T T L V W H L L R T P P E P P T P L P P E D R R Q S V S R Q P S F T Y S E W M E E K I E D D F L D L D P V P E T P V F D C V M D I K P E A D P T S L T V K S M G L Q E R R G S N V S L T L D M C T P G C N E E G F G Y L M S P R E E S A R E Y L L S A S R V L Q A E E L H E K A L D P F L L Q A E F F E I P M N F V D P K E Y D I P G L V R K N R Y K T I L P N P H S R V C L T S P D P D D P L S S Y I N A N Y I R G Y G G E E K V Y I A T Q G P I V S T V A D F W R M V W Q E H T P I I V M I T N I E E M N E K C T E Y W P E E Q V A Y D G V E I T V Q K V I H T E D Y R L R L I S L K S G T E E R G L K H Y W F T S W P D Q K T P D R A P P L L H L V R E V E E A A Q Q E G P H C A P I I V H C S A G I G R T G C F I A T S I C C Q Q L R Q E G V V D I L K T T C Q L R Q D R G G M I Q T C E Q Y Q F V H H V M S L Y E K Q L S H Q S P E
- Accession
- P54829
- Protein length
- Full Length
- Predicted molecular weight
- 89.9 kDa
- Amino acids
- 1 to 565
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
- Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.
- Post-translational modifications
Phosphorylation at Ser-245 by PKA deactivates PTPN5. Phosphorylation at Thr-255 and Ser-268 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STEP also known as PTPN5 is a protein tyrosine phosphatase with a molecular mass approximately 69-80 kDa. It regulates the activity of key signaling proteins by dephosphorylation. Expression of STEP occurs mainly in the central nervous system particularly in the striatum hippocampus and cortex regions of the brain. These areas of expression suggest its significant role in neuronal signaling and function. STEP exists in multiple isoforms which allow it to participate in various signaling pathways by altering substrate specificity.
- Biological function summary
STEP plays a significant role in modulating synaptic strength and plasticity acting as a negative regulator of synaptic signaling. Its main function involves dephosphorylating several kinase substrates like ERK1/2 and Fyn kinase thereby reducing their activity. This action controls synaptic sensitivity to neurotransmitters and impacts long-term potentiation a process essential for learning and memory. STEP does not form part of a large complex but interacts with other proteins through transient associations based on cellular needs.
- Pathways
STEP contributes significantly to the glutamatergic and dopaminergic signaling pathways. Within these pathways STEP dephosphorylates and inactivates specific receptors and kinases to maintain homeostasis in neuronal signaling. For example in the glutamatergic pathway STEP deactivates NMDA receptors affecting calcium influx and signaling cascades that rely on it. Its interactions with other proteins like ERK which is part of the MAPK pathway illustrate its integrated role in signal transduction cascades that influence cellular responses to neurotransmitters.
- Associated diseases and disorders
STEP has a relationship with neurodegenerative diseases such as Alzheimer's disease and schizophrenia. Increased activity of STEP leads to excessive dephosphorylation of proteins necessary for synaptic function contributing to the cognitive deficits observed in these disorders. In Alzheimer's disease for instance hyperactive STEP decreases the phosphorylation of the NMDA receptor and disrupts synaptic transmission. Additionally STEP's influence extends to dynamin-related protein 1 (Drp1) in neurodegenerative pathways indicating how altered STEP activity can escalate mitochondrial dysfunction commonly seen in Alzheimer's pathology.
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1 product image
SDS-PAGE - Recombinant Human STEP / PTPN5 protein (ab132095)
12.5% SDS-PAGE analysis of ab132095 stained with Coomassie Blue.
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