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AB132095

Recombinant Human STEP / PTPN5 protein (GST tag N-Terminus)

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Recombinant Human STEP / PTPN5 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 565 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Tyrosine-protein phosphatase non-receptor type 5, Neural-specific protein-tyrosine phosphatase, Striatum-enriched protein-tyrosine phosphatase, STEP, PTPN5

1 Images
SDS-PAGE - Recombinant Human STEP / PTPN5 protein (GST tag N-Terminus) (AB132095)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human STEP / PTPN5 protein (GST tag N-Terminus) (AB132095)

12.5% SDS-PAGE analysis of ab132095 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, SDS-PAGE, WB

applications

Biologically active

No

Accession

P54829

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB155329

Anti-STEP / PTPN5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEP / PTPN5 antibody (AB155329)

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Primary Antibodies

AB171870

Rabbit IgG, polyclonal - Isotype Control (ChIP Grade)

Western blot - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (AB171870)

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Primary Antibodies

AB37415

Rabbit IgG, polyclonal - Isotype Control

Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (AB37415)

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Primary Antibodies

AB208334

Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free)

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (AB208334)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MNYEGARSERENHAADDSEGGALDMCCSERLPGLPQPIVMEALDEAEGLQDSQREMPPPPPPSPPSDPAQKPPPRGAGSHSLTVRSSLCLFAASQFLLACGVLWFSGYGHIWSQNATNLVSSLLTLLKQLEPTAWLDSGTWGVPSLLLVFLSVGLVLVTTLVWHLLRTPPEPPTPLPPEDRRQSVSRQPSFTYSEWMEEKIEDDFLDLDPVPETPVFDCVMDIKPEADPTSLTVKSMGLQERRGSNVSLTLDMCTPGCNEEGFGYLMSPREESAREYLLSASRVLQAEELHEKALDPFLLQAEFFEIPMNFVDPKEYDIPGLVRKNRYKTILPNPHSRVCLTSPDPDDPLSSYINANYIRGYGGEEKVYIATQGPIVSTVADFWRMVWQEHTPIIVMITNIEEMNEKCTEYWPEEQVAYDGVEITVQKVIHTEDYRLRLISLKSGTEERGLKHYWFTSWPDQKTPDRAPPLLHLVREVEEAAQQEGPHCAPIIVHCSAGIGRTGCFIATSICCQQLRQEGVVDILKTTCQLRQDRGGMIQTCEQYQFVHHVMSLYEKQLSHQSPE","proteinLength":"Full Length","predictedMolecularWeight":"89.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":565,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P54829","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STEP also known as PTPN5 is a protein tyrosine phosphatase with a molecular mass approximately 69-80 kDa. It regulates the activity of key signaling proteins by dephosphorylation. Expression of STEP occurs mainly in the central nervous system particularly in the striatum hippocampus and cortex regions of the brain. These areas of expression suggest its significant role in neuronal signaling and function. STEP exists in multiple isoforms which allow it to participate in various signaling pathways by altering substrate specificity.
Biological function summary

STEP plays a significant role in modulating synaptic strength and plasticity acting as a negative regulator of synaptic signaling. Its main function involves dephosphorylating several kinase substrates like ERK1/2 and Fyn kinase thereby reducing their activity. This action controls synaptic sensitivity to neurotransmitters and impacts long-term potentiation a process essential for learning and memory. STEP does not form part of a large complex but interacts with other proteins through transient associations based on cellular needs.

Pathways

STEP contributes significantly to the glutamatergic and dopaminergic signaling pathways. Within these pathways STEP dephosphorylates and inactivates specific receptors and kinases to maintain homeostasis in neuronal signaling. For example in the glutamatergic pathway STEP deactivates NMDA receptors affecting calcium influx and signaling cascades that rely on it. Its interactions with other proteins like ERK which is part of the MAPK pathway illustrate its integrated role in signal transduction cascades that influence cellular responses to neurotransmitters.

STEP has a relationship with neurodegenerative diseases such as Alzheimer's disease and schizophrenia. Increased activity of STEP leads to excessive dephosphorylation of proteins necessary for synaptic function contributing to the cognitive deficits observed in these disorders. In Alzheimer's disease for instance hyperactive STEP decreases the phosphorylation of the NMDA receptor and disrupts synaptic transmission. Additionally STEP's influence extends to dynamin-related protein 1 (Drp1) in neurodegenerative pathways indicating how altered STEP activity can escalate mitochondrial dysfunction commonly seen in Alzheimer's pathology.
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Specifications

Form

Liquid

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General info

Function

May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.

Post-translational modifications

Phosphorylation at Ser-245 by PKA deactivates PTPN5. Phosphorylation at Thr-255 and Ser-268 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase.

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Product protocols

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Target data

May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
See full target information PTPN5
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Product promise

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