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AB127160

Recombinant Human STK16 protein

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(2 Publications)

Recombinant Human STK16 protein is a Human Full Length protein, in the 1 to 305 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

MPSK1, PKL12, TSF1, STK16, Serine/threonine-protein kinase 16, Myristoylated and palmitoylated serine/threonine-protein kinase, Protein kinase PKL12, TGF-beta-stimulated factor 1, Tyrosine-protein kinase STK16, hPSK, MPSK, TSF-1

1 Images
SDS-PAGE - Recombinant Human STK16 protein (AB127160)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human STK16 protein (AB127160)

SDS-PAGE analysis of ab127160.

Key facts

Purity

>95% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

O75716

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"58 kDa","actualMolecularWeight":null,"aminoAcidEnd":305,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O75716","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STK16 also known as Serine/threonine-protein kinase 16 or KRCT is a protein kinase that has a mass of approximately 33 kDa. It phosphorylates proteins on serine and threonine residues playing a role in several cellular processes. STK16 is expressed in many body tissues with higher levels detected in the brain liver and spleen. Its widespread expression suggests it has a function in different cellular contexts.
Biological function summary

STK16 influences various processes at the cellular level. It participates in vesicular trafficking and Golgi function ensuring proper sorting and delivery of proteins within cells. Although not typically seen as part of large complexes it interacts with several other proteins to exert its biological roles. The protein's activity affects cell cycle progression and can influence cellular growth and motility.

Pathways

STK16 integrates into key regulatory networks such as the Golgi-associated vesicle budding pathway and cell signaling pathways. These pathways are important for maintaining cellular organization and communication. STK16's interactions with proteins like COPII in vesicle trafficking and possibly components of the MAPK signaling cascade highlight its connectivity within cellular pathways and its regulatory potential.

STK16 has been associated with conditions related to cell cycle dysregulation and tumor growth. Dysfunctions in STK16 expression or activity might contribute to the progression of cancers such as breast cancer by impacting cell proliferation and survival. Other kinases like Akt may interact with STK16 or similar pathways influencing disease pathways and offering potential therapeutic intervention points.
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Specifications

Form

Liquid

Additional notes

Purity was determined to be >95% by densitometry. Affinity purified.

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General info

Function

Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Post-translational modifications

Mainly autophosphorylated on serine/threonine residues. Also autophosphorylated on Tyr-198.. It is uncertain whether palmitoylation is on Cys-6 and/or Cys-8.

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Product protocols

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Target data

Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins.
See full target information STK16
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 16:58 PubMed30851725

2019

Intrathecal triamcinolone acetonide exerts anti-inflammatory effects on Lewis rat experimental autoimmune neuritis and direct anti-oxidative effects on Schwann cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kalliopi Pitarokoili,Melissa Sgodzai,Thomas Grüter,Hussein Bachir,Jeremias Motte,Björn Ambrosius,Xiomara Pedreiturria,Min-Suk Yoon,Ralf Gold

Journal of neuroimmunology 309:58-67 PubMed28601289

2017

Anti-inflammatory and immunomodulatory potential of human immunoglobulin applied intrathecally in Lewis rat experimental autoimmune neuritis.

Applications

Unspecified application

Species

Unspecified reactive species

Kalliopi Pitarokoili,Felix Kohle,Jeremias Motte,Oluwaseun Fatoba,Xiomara Pedreiturria,Ralf Gold,Min-Suk Yoon
View all publications
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Complementary Products

Primary Antibodies

AB228608

Anti-STK16 antibody

Western blot - Anti-STK16 antibody (AB228608)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Secondary Antibodies

AB150191

Rabbit Anti-Human IgM mu chain (Alexa Fluor® 647)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Human IgM mu chain (Alexa Fluor® 647) (AB150191)

  • 0
  • 0
Secondary Antibodies

AB99811

Mouse monoclonal [JDC-12] Anti-Human lambda light chain (HRP)

VeriBlot

  • 5
  • 1

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