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AB139624

Recombinant human STK19/G11 protein

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Recombinant human STK19/G11 protein is a Human Full Length protein, in the 1 to 364 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

G11, RP1, STK19, TWH19, Winged helix repair factor 1, Inactive serine/threonine-protein kinase 19, Protein G11, Protein RP1, Tandem winged helix protein formerly known as STK19

2 Images
Functional Studies - Recombinant human STK19/G11 protein (AB139624)
  • FuncS

Supplier Data

Functional Studies - Recombinant human STK19/G11 protein (AB139624)

The specific activity of STK19/G11 (ab139624) was determined to be 1.7 nmol /min/mg as per activity assay protocol.

SDS-PAGE - Recombinant human STK19/G11 protein (AB139624)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human STK19/G11 protein (AB139624)

SDS PAGE analysis of ab139624

Key facts

Purity

>70% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab139624 was determined to be 2 nmol/min/mg.

Accession

P49842

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Complementary Products

Primary Antibodies

AB251814

Anti-STK19/G11 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK19/G11 antibody (AB251814)

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  • 0
Primary Antibodies

AB171978

Anti-TIMMDC1/C3orf1 antibody [EPR11807]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TIMMDC1/C3orf1 antibody [EPR11807] (AB171978)

  • 0
  • 0
Assay Kits

AB267631

Human HGFA ELISA Kit

Sandwich ELISA - Human HGFA ELISA Kit (AB267631)

  • 0
  • 0
Primary Antibodies

AB308612

Anti-CP2c antibody [14/LSF] - BSA and Azide free

Recombinant

Immunoprecipitation - Anti-CP2c antibody [14/LSF] - BSA and Azide free (AB308612)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

Previously labelled as STK19.

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Sequence info

[{"sequence":"MQKWFSAFDDAIIQRQWRANPSRGGGGVSFTKEVDTNVATGAPPRRQRVPGRACPWREPIRGRRGARPGGGDAGGTPGETVRHCSAPEDPIFRFSSLHSYPFPGTIKSRDMSWKRHHLIPETFGVKRRRKRGPVESDPLRGEPGSARAAVSELMQLFPRGLFEDALPPIVLRSQVYSLVPDRTVADRQLKELQEQGEIRIVQLGFDLDAHGIIFTEDYRTRVLKACDGRPYAGAVQKFLASVLPACGDLSFQQDQMTQTFGFRDSEITHLVNAGVLTVRDAGSWWLAVPGAGRFIKYFVKGRQAVLSMVRKAKYRELLLSELLGRRAPVVVRLGLTYHVHDLIGAQLVDCISTTSGTLLRLPET","proteinLength":"Full Length","predictedMolecularWeight":"70 kDa","actualMolecularWeight":null,"aminoAcidEnd":364,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P49842","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STK19 also known as G11 is a serine/threonine kinase that plays a mechanical role in phosphorylating specific protein substrates. It has an approximate molecular mass of 38 kDa. Expression of STK19 occurs in a variety of tissues with higher expression levels found in immune cells and certain areas of the brain. The kinase activity of STK19 modulates several cellular processes through phosphorylation acting as a regulatory switch for protein function.
Biological function summary

STK19 participates in signal transduction pathways by phosphorylating proteins that are involved in cell signaling and proliferation. It is part of a larger protein complex that includes scaffolding proteins which help in localizing and stabilizing STK19 within the cell. In immune cells STK19 plays a role in modulating the cellular immune response influencing both the adaptive and innate immune pathways.

Pathways

The action of STK19 is significant in the MAPK/ERK signaling pathway which is essential for cell growth and differentiation. This pathway interacts with proteins such as RAF1 and MEK1 where STK19's kinase activity is important for propagating signaling cascades that impact cellular outcomes. STK19 also interfaces with the PI3K/AKT pathway engaging in cell survival and metabolism regulation through phosphorylation events.

STK19 has been associated with autoimmune diseases and certain cancers. Aberrant expression or mutation of STK19 can contribute to immune system dysfunction linking it with proteins like STAT3 in the context of autoimmunity. Additionally STK19 mutations or dysregulation may influence oncogenic processes interacting with other key proteins such as p53 which is known to regulate cell cycle and tumorigenesis. Scientists continue to study STK19 to better understand its full role and potential as a therapeutic target in these disorders.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

DNA-binding protein which is required for efficient transcription-coupled nucleotide excision repair (TC-NER) (PubMed : 38252411, PubMed : 38890355, PubMed : 39353615, PubMed : 39547223, PubMed : 39547228, PubMed : 39547229). Acts as part of a TC-NER complex which assembles and interacts with RNA polymerase II (RNAPII) when it stalls at DNA lesions (PubMed : 39547223, PubMed : 39547228, PubMed : 39547229). TC-NER complex subunit UVSSA binds to the GTF2H1/p62 subunit of the TFIIH transcription factor complex, tethering TFIIH to the TC-NER complex (PubMed : 39547228). WHR1/STK19 then interacts with the XPD helicase subunit of TFIIH which guides TFIIH to DNA downstream of the stalled RNAPII, ensuring DNA repair (PubMed : 39353615, PubMed : 39547228, PubMed : 39547229). Directly interacts with RNAPII and also binds to downstream DNA (PubMed : 39547229). Promotes the timely removal of DNA damage-stalled RNAPII, allowing downstream NER factors to access DNA lesions (PubMed : 39547228, PubMed : 39547229). Required for monoubiquitination of UVSSA (PubMed : 39353615). Regulates repositioning and stabilization of UVSSA within the TC-NER complex (PubMed : 39547223). Stimulates ubiquitination of RNAPII complex member RBP1 (PubMed : 39547223). Also binds to RNA and regulates the expression levels of many mRNAs (PubMed : 38252411, PubMed : 38890355).

Sequence similarities

Belongs to the STK19 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

DNA-binding protein which is required for efficient transcription-coupled nucleotide excision repair (TC-NER) (PubMed : 38252411, PubMed : 38890355, PubMed : 39353615, PubMed : 39547223, PubMed : 39547228, PubMed : 39547229). Acts as part of a TC-NER complex which assembles and interacts with RNA polymerase II (RNAPII) when it stalls at DNA lesions (PubMed : 39547223, PubMed : 39547228, PubMed : 39547229). TC-NER complex subunit UVSSA binds to the GTF2H1/p62 subunit of the TFIIH transcription factor complex, tethering TFIIH to the TC-NER complex (PubMed : 39547228). WHR1/STK19 then interacts with the XPD helicase subunit of TFIIH which guides TFIIH to DNA downstream of the stalled RNAPII, ensuring DNA repair (PubMed : 39353615, PubMed : 39547228, PubMed : 39547229). Directly interacts with RNAPII and also binds to downstream DNA (PubMed : 39547229). Promotes the timely removal of DNA damage-stalled RNAPII, allowing downstream NER factors to access DNA lesions (PubMed : 39547228, PubMed : 39547229). Required for monoubiquitination of UVSSA (PubMed : 39353615). Regulates repositioning and stabilization of UVSSA within the TC-NER complex (PubMed : 39547223). Stimulates ubiquitination of RNAPII complex member RBP1 (PubMed : 39547223). Also binds to RNA and regulates the expression levels of many mRNAs (PubMed : 38252411, PubMed : 38890355).
See full target information STK19
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