Recombinant human STK25 protein is a Human Full Length protein, in the 1 to 426 aa range, expressed in Baculovirus infected Sf9, with =90% purity and suitable for SDS-PAGE, WB, FuncS.
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Key facts
- Purity
- =90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed:18782753).
Alternative names
SOK1, YSK1, STK25, Serine/threonine-protein kinase 25, Ste20-like kinase, Sterile 20/oxidant stress-response kinase 1, SOK-1, Ste20/oxidant stress response kinase 1
Recommended products
Recombinant human STK25 protein is a Human Full Length protein, in the 1 to 426 aa range, expressed in Baculovirus infected Sf9, with =90% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- =90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab125588 was determined to be 11 nmol/min/mg.
- Accession
- O00506-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- Accession
- O00506
- Protein length
- Full Length
- Predicted molecular weight
- 76 kDa
- Amino acids
- 1 to 426
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed:18782753).
- Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Native Pig Myelin Basic Protein ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STK25 also known as Serine/Threonine Kinase 25 or YSK1 is an enzyme with a molecular weight of approximately 59 kDa. It functions as a serine/threonine kinase and plays a role in the regulation of various cellular processes. STK25 is present in many tissues but its expression levels are high in the brain liver and testis. This kinase is involved in several signaling pathways that modulate cell dynamics and organization influencing cellular architecture and function.
- Biological function summary
STK25 participates in signaling pathways that govern structural cellular dynamics. It acts independently or as part of a larger protein complex to control cell polarity and morphological changes. STK25 can influence actin cytoskeleton reorganization which affects cell shape and movement. It holds importance for processes related to cell adhesion and cell-matrix interactions important for maintaining proper cell structures and responses to environmental stimuli.
- Pathways
STK25 integrates into key signaling cascades. It plays a role in the Hippo signaling pathway which regulates organ size and suppresses tumor growth. STK25 interacts with other proteins such as MST1/2 to influence this pathway's activity. Additionally it associates with the MAPK signaling pathway modulating cellular responses like proliferation and stress resistance. Through these interactions STK25 connects to broader networks influencing cellular behavior and responses.
- Associated diseases and disorders
STK25 has links to neurodegenerative disorders and diabetes. Studies have shown its contribution to the development of Alzheimer's disease where it may interact with tau protein leading to tau hyperphosphorylation and aggregation. Moreover STK25 relates to metabolic syndromes particularly insulin resistance by associating with pathways affecting glucose homeostasis. These relationships suggest STK25's involvement in both neurological and metabolic health.
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2 product images
SDS-PAGE - Recombinant human STK25 protein (ab125588)
SDS Page analysis of ab125588
Functional Studies - Recombinant human STK25 protein (ab125588)
The specific activity of ab125588 was determined to be 11 nmol/min/mg
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