Recombinant human STK3/MST-2 protein is a Human Full Length protein, in the 2 to 491 aa range, expressed in Baculovirus infected Sf9, with >41% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >41% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
E Q P P A P K S K L K K L S E D S L T K Q P E E V F D V L E K L G E G S Y G S V F K A I H K E S G Q V V A I K Q V P V E S D L Q E I I K E I S I M Q Q C D S P Y V V K Y Y G S Y F K N T D L W I V M E Y C G A G S V S D I I R L R N K T L I E D E I A T I L K S T L K G L E Y L H F M R K I H R D I K A G N I L L N T E G H A K L A D F G V A G Q L T D T M A K R N T V I G T P F W M A P E V I Q E I G Y N C V A D I W S L G I T S I E M A E G K P P Y A D I H P M R A I F M I P T N P P P T F R K P E L W S D D F T D F V K K C L V K N P E Q R A T A T Q L L Q H P F I K N A K P V S I L R D L I T E A M E I K A K R H E E Q Q R E L E E E E E N S D E D E L D S H T M V K T S V E S V G T M R A T S T M S E G A Q T M I E H N S T M L E S D L G T M V I N S E D E E E E D G T M K R N A T S P Q V Q R P S F M D Y F D K Q D F K N K S H E N C N Q N M H E P F P M S K N V F P D N W K V P Q D G D F D F L K N L S L E E L Q M R L K A L D P M M E R E I E E L R Q R Y T A K R Q P I L D A M D A K K R R Q Q N F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation (PubMed:11278283, PubMed:8566796, PubMed:8816758). Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:15688006, PubMed:16930133, PubMed:23972470, PubMed:28087714, PubMed:29063833, PubMed:30622739). Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:15688006, PubMed:16930133, PubMed:23972470, PubMed:28087714). STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250 (PubMed:21076410, PubMed:21723128). In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation (PubMed:21104395). Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259' (PubMed:20212043). Phosphorylates MOBKL1A and RASSF2 (PubMed:19525978). Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38 (PubMed:18328708, PubMed:18362890).
Alternative names
KRS1, MST2, STK3, Serine/threonine-protein kinase 3, Mammalian STE20-like protein kinase 2, STE20-like kinase MST2, Serine/threonine-protein kinase Krs-1, MST-2
Recommended products
Recombinant human STK3/MST-2 protein is a Human Full Length protein, in the 2 to 491 aa range, expressed in Baculovirus infected Sf9, with >41% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >41% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity of ab195160 was determined to be 31 pmol/min/μg. Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35 and 2 μM substrate (Ser/Thr Peptide 7) at 30°C for 1 hour.
- Accession
- Q13188-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 3% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.72% Sodium chloride, 0.71% Tris HCl, 0.17% Glutathione, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- E Q P P A P K S K L K K L S E D S L T K Q P E E V F D V L E K L G E G S Y G S V F K A I H K E S G Q V V A I K Q V P V E S D L Q E I I K E I S I M Q Q C D S P Y V V K Y Y G S Y F K N T D L W I V M E Y C G A G S V S D I I R L R N K T L I E D E I A T I L K S T L K G L E Y L H F M R K I H R D I K A G N I L L N T E G H A K L A D F G V A G Q L T D T M A K R N T V I G T P F W M A P E V I Q E I G Y N C V A D I W S L G I T S I E M A E G K P P Y A D I H P M R A I F M I P T N P P P T F R K P E L W S D D F T D F V K K C L V K N P E Q R A T A T Q L L Q H P F I K N A K P V S I L R D L I T E A M E I K A K R H E E Q Q R E L E E E E E N S D E D E L D S H T M V K T S V E S V G T M R A T S T M S E G A Q T M I E H N S T M L E S D L G T M V I N S E D E E E E D G T M K R N A T S P Q V Q R P S F M D Y F D K Q D F K N K S H E N C N Q N M H E P F P M S K N V F P D N W K V P Q D G D F D F L K N L S L E E L Q M R L K A L D P M M E R E I E E L R Q R Y T A K R Q P I L D A M D A K K R R Q Q N F
- Accession
- Q13188
- Protein length
- Full Length
- Predicted molecular weight
- 78.4 kDa
- Amino acids
- 2 to 491
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation (PubMed:11278283, PubMed:8566796, PubMed:8816758). Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:15688006, PubMed:16930133, PubMed:23972470, PubMed:28087714, PubMed:29063833, PubMed:30622739). Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:15688006, PubMed:16930133, PubMed:23972470, PubMed:28087714). STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250 (PubMed:21076410, PubMed:21723128). In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation (PubMed:21104395). Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259' (PubMed:20212043). Phosphorylates MOBKL1A and RASSF2 (PubMed:19525978). Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38 (PubMed:18328708, PubMed:18362890).
- Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
- Post-translational modifications
Autophosphorylated on two residues Thr-174 and Thr-180, leading to activation (PubMed:23972470, PubMed:29063833). Phosphorylation at Thr-117 and Thr-384 by PKB/AKT1, leads to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation, and increase in its binding to RAF1. Phosphorylated at Ser-15 by PLK1, leading to activation (PubMed:21723128). When autophosphorylated at Thr-180, recruits STRIPAK complex and promotes PP2A-mediated dephosphorylation and inactivation of STK3 (PubMed:29063833, PubMed:30622739).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Previously labelled as STK3.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STK3 also known as MST-2 is a serine/threonine protein kinase with a molecular mass of around 60 kDa. It plays an integral role in cell signaling by phosphorylating and activating downstream targets. MST-2 localizes mainly in the cytoplasm and is expressed broadly across various tissues with notable activity in liver kidney and heart tissues. This kinase contributes to the regulation of cell shape and apoptosis reinforcing its importance in cellular homeostasis.
- Biological function summary
STK3/MST-2 is a core component of the Hippo signaling pathway which controls organ size by regulating cell proliferation and apoptosis. MST-2 often interacts with other proteins and participates in functional complexes notably with MOB1 and LATS1/2. These interactions are essential for transmitting growth inhibitory signals. Through its kinase activity MST-2 phosphorylates and activates these molecules initiating a cascade that ultimately influences gene expression impacting cell cycle and cell death processes.
- Pathways
MST-2 plays a pivotal role not only in the Hippo pathway but also in the MAPK signaling network. In the Hippo pathway MST-2 phosphorylates components like LATS1/2 subsequently affecting the translocation and activity of YAP/TAZ transcription factors which control cell proliferation and survival. In the MAPK pathway MST-2 can interact with other kinases such as ASK1 to influence stress response signaling. These interactions demonstrate how MST-2 integrates signals between pathways to mediate critical decisions in cell life and death.
- Associated diseases and disorders
MST-2 has implications in cancer and cardiac diseases. Dysregulation of the Hippo pathway involving MST-2 is frequently observed in various cancers where impaired MST-2 function contributes to unchecked cell growth and tumor progression. Moreover MST-2 connects with other proteins like YAP often overactive in cancers emphasizing its role in controlling cell division. In cardiac context alterations in MST-2 activity relate to cardiac hypertrophy as proper functioning of the kinase is necessary to maintain cardiac tissue architecture and response to stress.
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2 product images
Functional Studies - Recombinant human STK3/MST-2 protein (ab195160)
Specific activity of ab195160 was determined to be 31 pmol/min/μg.
SDS-PAGE - Recombinant human STK3/MST-2 protein (ab195160)
10% SDS-PAGE using of 5 μg ab195160 stained with Coomassie Blue.
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