Recombinant human STK36 protein is a Human Fragment protein, in the 1 to 457 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, WB, FuncS.
Images
Key facts
- Purity
- >80% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M E K Y H V L E M I G E G S F G R V Y K G R R K Y S A Q V V A L K F I P K L G R S E K E L R N L Q R E I E I M R G L R H P N I V H M L D S F E T D K E V V V V T D Y A E G E L F Q I L E D D G K L P E D Q V Q A I A A Q L V S A L Y Y L H S H R I L H R D M K P Q N I L L A K G G G I K L C D F G F A R A M S T N T M V L T S I K G T P L Y M S P E L V E E R P Y D H T A D L W S V G C I L Y E L A V G T P P F Y A T S I F Q L V S L I L K D P V R W P S T I S P C F K N F L Q G L L T K D P R Q R L S W P D L L Y H P F I A G H V T I I T E P A G P D L G T P F T S R L P P E L Q V L K D E Q A H R L A P K G N Q S R I L T Q A Y K R M A E E A M Q K K H Q N T G P A L E Q E D K T S K V A P G T A P L P R L G A T P Q E S S L L A G I L A S E L K S S W A K S G T G E V P S A P R E N R T T P D C E R A F P E E R P E V L G Q R S T D V V D L E N E E P D S D N E W Q H L L E T T E P V P I Q L K A P L T L L C N P D F C Q R I Q S Q L H E A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serine/threonine protein kinase which plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors (PubMed:10806483). Controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization (PubMed:10806483). GLI2 requires an additional function of STK36 to become transcriptionally active, but the enzyme does not need to possess an active kinase catalytic site for this to occur (PubMed:10806483). Required for postnatal development, possibly by regulating the homeostasis of cerebral spinal fluid or ciliary function. Essential for construction of the central pair apparatus of motile cilia.
Alternative names
KIAA1278, STK36, Serine/threonine-protein kinase 36, Fused homolog
Recommended products
Recombinant human STK36 protein is a Human Fragment protein, in the 1 to 457 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >80% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab139615 was determined to be 0.6 nmol/min/mg.
- Accession
- Q9NRP7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M E K Y H V L E M I G E G S F G R V Y K G R R K Y S A Q V V A L K F I P K L G R S E K E L R N L Q R E I E I M R G L R H P N I V H M L D S F E T D K E V V V V T D Y A E G E L F Q I L E D D G K L P E D Q V Q A I A A Q L V S A L Y Y L H S H R I L H R D M K P Q N I L L A K G G G I K L C D F G F A R A M S T N T M V L T S I K G T P L Y M S P E L V E E R P Y D H T A D L W S V G C I L Y E L A V G T P P F Y A T S I F Q L V S L I L K D P V R W P S T I S P C F K N F L Q G L L T K D P R Q R L S W P D L L Y H P F I A G H V T I I T E P A G P D L G T P F T S R L P P E L Q V L K D E Q A H R L A P K G N Q S R I L T Q A Y K R M A E E A M Q K K H Q N T G P A L E Q E D K T S K V A P G T A P L P R L G A T P Q E S S L L A G I L A S E L K S S W A K S G T G E V P S A P R E N R T T P D C E R A F P E E R P E V L G Q R S T D V V D L E N E E P D S D N E W Q H L L E T T E P V P I Q L K A P L T L L C N P D F C Q R I Q S Q L H E A
- Accession
- Q9NRP7
- Protein length
- Fragment
- Predicted molecular weight
- 87 kDa
- Amino acids
- 1 to 457
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Serine/threonine protein kinase which plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors (PubMed:10806483). Controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization (PubMed:10806483). GLI2 requires an additional function of STK36 to become transcriptionally active, but the enzyme does not need to possess an active kinase catalytic site for this to occur (PubMed:10806483). Required for postnatal development, possibly by regulating the homeostasis of cerebral spinal fluid or ciliary function. Essential for construction of the central pair apparatus of motile cilia.
- Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Native Pig Myelin Basic Protein ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STK36 also known as serine/threonine kinase 36 is a protein with a molecular weight of approximately 134 kDa. It functions primarily as a kinase transferring phosphate groups from ATP to serine or threonine residues on target proteins. STK36 is expressed in various tissues with higher expression levels observed in the brain and testis. This expression suggests a significant role in cellular processes in these areas. STK36's activity is important for maintaining proper signaling pathways within cells.
- Biological function summary
STK36 plays an essential role in the sonic hedgehog (SHH) signaling pathway which regulates embryonic development and cell differentiation. It acts as part of a complex that includes GLI transcription factors influencing their activity through phosphorylation. By regulating the activation of GLI proteins STK36 affects the transcription of genes involved in growth and morphogenesis. This regulatory mechanism highlights its influence on cellular development and division.
- Pathways
The protein interacts mainly within the SHH signaling axis. It stabilizes the activity of the GLI transcription factors important for the expression of genes like PTCH1 and BCL2. Additionally STK36 operates within the context of the primary cilia a cellular structure where it works alongside proteins such as Smoothened (SMO) to mediate signal transduction. Through its pathway interactions STK36 directly affects the development and function of various cellular processes.
- Associated diseases and disorders
Mutations or dysregulation of STK36 have been linked to conditions such as medulloblastoma and hydrocephalus. In these contexts its interactions with proteins like the Suppressor of Fused (SUFU) become disrupted leading to aberrant SHH pathway activity. These diseases highlight the importance of STK36 in maintaining normal cellular signaling illustrating how its disruption can have profound pathological effects.
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3 product images
Functional Studies - Recombinant human STK36 protein (ab139615)
The specific activity of STK36 (ab139615) was determined to be 0.6 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human STK36 protein (ab139615)
SDS PAGE analysis of ab139615
SDS-PAGE - Recombinant human STK36 protein (ab139615)
SDS PAGE analysis of ab139615
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