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AB139615

Recombinant human STK36 protein

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Recombinant human STK36 protein is a Human Fragment protein, in the 1 to 457 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

KIAA1278, STK36, Serine/threonine-protein kinase 36, Fused homolog

3 Images
Functional Studies - Recombinant human STK36 protein (AB139615)
  • FuncS

Unknown

Functional Studies - Recombinant human STK36 protein (AB139615)

The specific activity of STK36 (ab139615) was determined to be 0.6 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human STK36 protein (AB139615)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human STK36 protein (AB139615)

SDS PAGE analysis of ab139615

SDS-PAGE - Recombinant human STK36 protein (AB139615)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human STK36 protein (AB139615)

SDS PAGE analysis of ab139615

Key facts

Purity

>80% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, WB

applications

Biologically active

Yes

Biological activity

The specific activity of ab139615 was determined to be 0.6 nmol/min/mg.

Accession

Q9NRP7

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"sequence":"MEKYHVLEMIGEGSFGRVYKGRRKYSAQVVALKFIPKLGRSEKELRNLQREIEIMRGLRHPNIVHMLDSFETDKEVVVVTDYAEGELFQILEDDGKLPEDQVQAIAAQLVSALYYLHSHRILHRDMKPQNILLAKGGGIKLCDFGFARAMSTNTMVLTSIKGTPLYMSPELVEERPYDHTADLWSVGCILYELAVGTPPFYATSIFQLVSLILKDPVRWPSTISPCFKNFLQGLLTKDPRQRLSWPDLLYHPFIAGHVTIITEPAGPDLGTPFTSRLPPELQVLKDEQAHRLAPKGNQSRILTQAYKRMAEEAMQKKHQNTGPALEQEDKTSKVAPGTAPLPRLGATPQESSLLAGILASELKSSWAKSGTGEVPSAPRENRTTPDCERAFPEERPEVLGQRSTDVVDLENEEPDSDNEWQHLLETTEPVPIQLKAPLTLLCNPDFCQRIQSQLHEA","proteinLength":"Fragment","predictedMolecularWeight":"87 kDa","actualMolecularWeight":null,"aminoAcidEnd":457,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9NRP7","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STK36 also known as serine/threonine kinase 36 is a protein with a molecular weight of approximately 134 kDa. It functions primarily as a kinase transferring phosphate groups from ATP to serine or threonine residues on target proteins. STK36 is expressed in various tissues with higher expression levels observed in the brain and testis. This expression suggests a significant role in cellular processes in these areas. STK36's activity is important for maintaining proper signaling pathways within cells.
Biological function summary

STK36 plays an essential role in the sonic hedgehog (SHH) signaling pathway which regulates embryonic development and cell differentiation. It acts as part of a complex that includes GLI transcription factors influencing their activity through phosphorylation. By regulating the activation of GLI proteins STK36 affects the transcription of genes involved in growth and morphogenesis. This regulatory mechanism highlights its influence on cellular development and division.

Pathways

The protein interacts mainly within the SHH signaling axis. It stabilizes the activity of the GLI transcription factors important for the expression of genes like PTCH1 and BCL2. Additionally STK36 operates within the context of the primary cilia a cellular structure where it works alongside proteins such as Smoothened (SMO) to mediate signal transduction. Through its pathway interactions STK36 directly affects the development and function of various cellular processes.

Mutations or dysregulation of STK36 have been linked to conditions such as medulloblastoma and hydrocephalus. In these contexts its interactions with proteins like the Suppressor of Fused (SUFU) become disrupted leading to aberrant SHH pathway activity. These diseases highlight the importance of STK36 in maintaining normal cellular signaling illustrating how its disruption can have profound pathological effects.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Serine/threonine protein kinase which plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors (PubMed : 10806483). Controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization (PubMed : 10806483). GLI2 requires an additional function of STK36 to become transcriptionally active, but the enzyme does not need to possess an active kinase catalytic site for this to occur (PubMed : 10806483). Required for postnatal development, possibly by regulating the homeostasis of cerebral spinal fluid or ciliary function. Essential for construction of the central pair apparatus of motile cilia.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Serine/threonine protein kinase which plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors (PubMed : 10806483). Controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization (PubMed : 10806483). GLI2 requires an additional function of STK36 to become transcriptionally active, but the enzyme does not need to possess an active kinase catalytic site for this to occur (PubMed : 10806483). Required for postnatal development, possibly by regulating the homeostasis of cerebral spinal fluid or ciliary function. Essential for construction of the central pair apparatus of motile cilia.
See full target information STK36
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Complementary Products

Primary Antibodies

AB103807

Anti-STK36 antibody

Western blot - Anti-STK36 antibody (AB103807)

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