Recombinant Human STRAD protein (denatured) is a Human Full Length protein, in the 1 to 314 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S F L V S K P E R I R T N D A S S E S I A S F S K Q E V M S S F L P E G G C Y E L L T V I G K G F E D L M T V N L A R Y K P T G E Y V T V R R I N L E A C S N E M V T F L Q G E L H V S K L F N H P N I V P Y R A T F I A D N E L W V V T S F M A Y G S A K D L I C T H F M D G M N E L A I A Y I L Q G V L K A L D Y I H H M G Y V H R S V K A S H I L I S V D G K V Y L S G L R S N L S M I S H G Q R Q R V V H D F P K Y S V K V L P W L S P E V L Q Q N L Q G Y D A K S D I Y S V G I T A C E L A N G H V P F K D M P A T Q M L L E K L N G T V P C L L D T S T I P A E E L T M S P S R S V A N S G L S D S L T T S T P R P S N G P V P A P S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.
Alternative names
LYK5, STRAD, STRADA, STE20-related kinase adapter protein alpha, STRAD alpha, STE20-related adapter protein, Serologically defined breast cancer antigen NY-BR-96
Recommended products
Recombinant Human STRAD protein (denatured) is a Human Full Length protein, in the 1 to 314 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q7RTN6-6
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S F L V S K P E R I R T N D A S S E S I A S F S K Q E V M S S F L P E G G C Y E L L T V I G K G F E D L M T V N L A R Y K P T G E Y V T V R R I N L E A C S N E M V T F L Q G E L H V S K L F N H P N I V P Y R A T F I A D N E L W V V T S F M A Y G S A K D L I C T H F M D G M N E L A I A Y I L Q G V L K A L D Y I H H M G Y V H R S V K A S H I L I S V D G K V Y L S G L R S N L S M I S H G Q R Q R V V H D F P K Y S V K V L P W L S P E V L Q Q N L Q G Y D A K S D I Y S V G I T A C E L A N G H V P F K D M P A T Q M L L E K L N G T V P C L L D T S T I P A E E L T M S P S R S V A N S G L S D S L T T S T P R P S N G P V P A P S
- Accession
- Q7RTN6
- Protein length
- Full Length
- Predicted molecular weight
- 37 kDa
- Amino acids
- 1 to 314
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.
- Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as LYK5
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The STE20-related adaptor (STRAD) is a pseudokinase that functions in cellular processes by regulating the LKB1 kinase activity. Also known as STRADA its molecular mass varies but it typically weighs around 50 kDa. STRAD is expressed in various tissues including the brain liver and muscle. This wide expression suggests it plays multiple roles in cellular function.
- Biological function summary
STRAD interacts with the serine/threonine-protein kinase LKB1 to promote its catalytic activity. It forms a complex with LKB1 and MO25 which stabilizes LKB1 in an active conformation. This complex plays an important role in regulating cellular energy balance and cell polarity. STRAD’s interaction is essential for localizing LKB1 to the cytoplasm where LKB1 exerts its function.
- Pathways
STRAD plays a significant role in the AMP-activated protein kinase (AMPK) pathway which is important for maintaining energy homeostasis. It helps activate AMPK through its relationship with LKB1. STRAD is also involved in the PI3K/AKT pathway which affects cell growth and survival demonstrating its impact on critical signaling processes. The interplay of STRAD with these pathways involves interactions with related proteins such as AMPK subunits and AKT.
- Associated diseases and disorders
Malfunction or mutation of the STRAD protein has been implicated in Peutz-Jeghers syndrome a disorder characterized by gastrointestinal polyps and an increased cancer risk. This association is largely due to its interaction with LKB1 mutations of which are known to drive the disease. STRAD alterations have also been associated with certain forms of epilepsy given its role in neuron function and brain expression. The involvement in these health conditions underlines the importance of STRAD in human health.
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1 product image
SDS-PAGE - Recombinant Human STRAD protein (denatured) (ab181925)
15% SDS PAGE analysis of ab181925 (3μg).
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