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AB93936

Recombinant Human SULT2B1 protein (Tag Free)

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Recombinant Human SULT2B1 protein (Tag Free) is a Human Full Length protein, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

HSST2, SULT2B1, Sulfotransferase 2B1, Alcohol sulfotransferase, Hydroxysteroid sulfotransferase 2, Sulfotransferase family 2B member 1, Sulfotransferase family cytosolic 2B member 1, ST2B1

1 Images
SDS-PAGE - Recombinant Human SULT2B1 protein (Tag Free) (AB93936)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SULT2B1 protein (Tag Free) (AB93936)

15% SDS-PAGE analysis of ab93936 (3μg)

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O00204

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Complementary Products

Primary Antibodies

AB276055

Anti-SULT2B1 antibody [007] - BSA and Azide free

Recombinant

Immunoprecipitation - Anti-SULT2B1 antibody [007] - BSA and Azide free (AB276055)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB196235

Anti-Mesothelin antibody [EPR19025-42]

RabMAb|Recombinant

Flow Cytometry - Anti-Mesothelin antibody [EPR19025-42] (AB196235)

  • 0
  • 0
Primary Antibodies

AB87117

Anti-ALDH1L1 antibody - Astrocyte Marker

Lab Essentials

Western blot - Anti-ALDH1L1 antibody - Astrocyte Marker (AB87117)

  • 4
  • 11
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MDGPAEPQIPGLWDTYEDDISEISQKLPGEYFRYKGVPFPVGLYSLESISLAENTQDVRDDDIFIITYPKSGTTWMIEIICLILKEGDPSWIRSVPIWERAPWCETIVGAFSLPDQYSPRLMSSHLPIQIFTKAFFSSKAKVIYMGRNPRDVVVSLYHYSKIAGQLKDPGTPDQFLRDFLKGEVQFGSWFDHIKGWLRMKGKDNFLFITYEELQQDLQGSVERICGFLGRPLGKEALGSVVAHSTFSAMKANTMSNYTLLPPSLLDHRRGAFLRKGVCGDWKNHFTVAQSEAFDRAYRKQMRGMPTFPWDEDPEEDGSPDPEPSPEPEPKPSLEPNTSLEREPRPNSSPSPSPGQASETPHPRPS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O00204","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SULT2B1 also known as sulfotransferase family 2B member 1 functions as a cytosolic enzyme that adds sulfate groups to various molecules. This post-translational modification is important for the metabolism of hormones neurotransmitters drugs and xenobiotic compounds. SULT2B1 has a molecular mass of approximately 42 kDa. It is expressed in a variety of tissues with higher levels in the skin brain and reproductive organs indicating its diverse physiological roles across different systems in the body.
Biological function summary

The role of SULT2B1 involves the sulfation of cholesterol and dehydroepiandrosterone (DHEA) which are important for maintaining lipid homeostasis and steroid hormone biosynthesis. The enzyme is not part of a complex but works optimally in its standalone cytosolic environment. It participates actively in regulating the availability of active hormones and lipids making it a significant player in cellular metabolic processes.

Pathways

SULT2B1 operates within two main biological frameworks: the steroid hormone biosynthesis pathway and lipid metabolic pathway. In these pathways the enzyme interacts closely with cholesterol and DHEA to modulate their sulfation status influencing precursor availability for steroidogenesis. It works in conjunction with other sulfotransferases like SULT1A1 that also help manage the sulfate pool important for hormone regulation and metabolic balance.

Aberrations in SULT2B1 function can link to conditions like polycystic ovary syndrome (PCOS) and certain cancers due to its role in steroid hormone metabolism. Disruptions in the enzyme's activity could alter DHEA and cholesterol sulfation leading to hormone imbalances that contribute to these disorders. In the context of PCOS associated enzymes might include subtypes of cytochrome P450 which function alongside SULT2B1 in steroid biosynthesis pathways to maintain hormone equilibrium.
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Specifications

Form

Liquid

Additional notes

Purified using conventional chromatography techniques.

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General info

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation. Responsible for the sulfation of cholesterol (PubMed : 12145317, PubMed : 19589875). Catalyzes sulfation of the 3beta-hydroxyl groups of steroids, such as, pregnenolone and dehydroepiandrosterone (DHEA) (PubMed : 12145317, PubMed : 16855051, PubMed : 21855633, PubMed : 9799594). Preferentially sulfonates cholesterol, while it has also significant activity with pregnenolone and DHEA (PubMed : 12145317, PubMed : 21855633). Plays a role in epidermal cholesterol metabolism and in the regulation of epidermal proliferation and differentiation (PubMed : 28575648).. Isoform 2. Sulfonates pregnenolone but not cholesterol.

Sequence similarities

Belongs to the sulfotransferase 1 family.

Post-translational modifications

Phosphorylated.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation. Responsible for the sulfation of cholesterol (PubMed : 12145317, PubMed : 19589875). Catalyzes sulfation of the 3beta-hydroxyl groups of steroids, such as, pregnenolone and dehydroepiandrosterone (DHEA) (PubMed : 12145317, PubMed : 16855051, PubMed : 21855633, PubMed : 9799594). Preferentially sulfonates cholesterol, while it has also significant activity with pregnenolone and DHEA (PubMed : 12145317, PubMed : 21855633). Plays a role in epidermal cholesterol metabolism and in the regulation of epidermal proliferation and differentiation (PubMed : 28575648).. Isoform 2. Sulfonates pregnenolone but not cholesterol.
See full target information SULT2B1
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