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AB140420

Recombinant Human Sumo 2 protein

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Recombinant Human Sumo 2 protein is a Human Full Length protein, in the 1 to 93 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

SMT3B, SMT3H2, SUMO2, Small ubiquitin-related modifier 2, SUMO-2, HSMT3, SMT3 homolog 2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Smt3B

5 Images
Western blot - Recombinant Human Sumo 2 protein (AB140420)
  • WB

Lab

Western blot - Recombinant Human Sumo 2 protein (AB140420)

**Blocking buffer : ** 5% NFDM/TBST This data was developed using ab109005, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (<a href='/en-us/products/primary-antibodies/sumo-2-sumo-3-antibody-epr4602-ab109005'>ab109005</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human Sumo 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-sumo-1-protein-ab140417'>ab140417</a>) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg

Lane 3:

Recombinant Human Sumo 3 protein (<a href='/en-us/products/unavailable/recombinant-human-sumo-3-protein-ab140414'>ab140414</a>) at 0.01 µg

Lane 4:

Western blot - Recombinant Human Sumo 4 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-sumo-4-protein-ab157025'>ab157025</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 16 kDa

true

Exposure time: 20s

Western blot - Recombinant Human Sumo 2 protein (AB140420)
  • WB

Lab

Western blot - Recombinant Human Sumo 2 protein (AB140420)

**Blocking buffer : ** 5% NFDM/TBST This data was developed using ab109196, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (<a href='/en-us/products/primary-antibodies/sumo-2-sumo-3-sumo-4-antibody-epr3002-ab109196'>ab109196</a>) at 1/200 dilution

Lane 1:

Western blot - Recombinant Human Sumo 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-sumo-1-protein-ab140417'>ab140417</a>) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg

Lane 3:

Recombinant Human Sumo 3 protein (<a href='/en-us/products/unavailable/recombinant-human-sumo-3-protein-ab140414'>ab140414</a>) at 0.01 µg

Lane 4:

Western blot - Recombinant Human Sumo 4 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-sumo-4-protein-ab157025'>ab157025</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 16 kDa

true

Exposure time: 20s

Western blot - Recombinant Human Sumo 2 protein (AB140420)
  • WB

Lab

Western blot - Recombinant Human Sumo 2 protein (AB140420)

**Blocking buffer : ** 5% NFDM/TBST This data was developed using ab133352, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sumo 1 antibody [EP298] (<a href='/en-us/products/primary-antibodies/sumo-1-antibody-ep298-ab133352'>ab133352</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human Sumo 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-sumo-1-protein-ab140417'>ab140417</a>) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg

Lane 3:

Recombinant Human Sumo 3 protein (<a href='/en-us/products/unavailable/recombinant-human-sumo-3-protein-ab140414'>ab140414</a>) at 0.01 µg

Lane 4:

Western blot - Recombinant Human Sumo 4 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-sumo-4-protein-ab157025'>ab157025</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

Observed band size: 16 kDa

true

Exposure time: 10s

Western blot - Recombinant Human Sumo 2 protein (AB140420)
  • WB

Lab

Western blot - Recombinant Human Sumo 2 protein (AB140420)

**Blocking buffer : ** 5% NFDM/TBST This data was developed using ab126606, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (<a href='/en-us/products/primary-antibodies/sumo-2-sumo-3-sumo-4-antibody-epr7163-ab126606'>ab126606</a>) at 1/5000 dilution

Lane 1:

Western blot - Recombinant Human Sumo 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-sumo-1-protein-ab140417'>ab140417</a>) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg

Lane 3:

Recombinant Human Sumo 3 protein (<a href='/en-us/products/unavailable/recombinant-human-sumo-3-protein-ab140414'>ab140414</a>) at 0.01 µg

Lane 4:

Western blot - Recombinant Human Sumo 4 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-sumo-4-protein-ab157025'>ab157025</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

Observed band size: 16 kDa

true

Exposure time: 3s

SDS-PAGE - Recombinant Human Sumo 2 protein (AB140420)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Sumo 2 protein (AB140420)

SDS-PAGE analysis of ab140420.

Key facts

Purity

>95% Densitometry

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P61956

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

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Complementary Products

Reagents, Controls & Accessories

AB325996

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 12 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 12 bands) (AB325996)

  • 0
  • 0
Proteins & Peptides

AB140417

Recombinant Human Sumo 1 protein

Western blot - Recombinant Human Sumo 1 protein (AB140417)

  • 0
  • 0
Proteins & Peptides

AB269103

Recombinant human UBE2I / UBC9 protein (Active)

Functional Studies - Recombinant human UBE2I / UBC9 protein (Active) (AB269103)

  • 0
  • 0
Primary Antibodies

AB81371

Anti-Sumo 2 + Sumo 3 antibody [8A2]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] (AB81371)

  • 4
  • 7
Proteins & Peptides

AB269130

Recombinant human VCP protein (Active)

Functional Studies - Recombinant human VCP protein (Active) (AB269130)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG","proteinLength":"Full Length","predictedMolecularWeight":"17.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":93,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P61956","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sumo 2 also known as SUMO-2 or SMT3A is a small ubiquitin-like modifier protein with a molecular mass of approximately 11 kDa. It belongs to the SUMO protein family which includes SUMO-1 SUMO-3 and others. Sumo 2 is widely expressed in the nucleus and cytoplasm of eukaryotic cells. It is involved in the post-translational modification process known as SUMOylation where it covalently attaches to specific lysine residues on substrate proteins influencing their activity localization and stability.
Biological function summary

Sumo 2 influences various cellular processes including nuclear-cytosolic transport transcriptional regulation and protein stability. It often forms part of multi-protein complexes showing versatility in regulating cellular responses to stress and maintaining protein homeostasis. Sumo 2's dynamic attachment and detachment from its target proteins allow fine-tuning of cellular pathways reflecting its essential role in managing cellular conditions.

Pathways

Sumo 2 participates in the DNA damage response and the heat shock response pathways. Its involvement helps ensure proper cell cycle progression and stress response through interaction with key proteins like p53 and HSF1. Sumo 2 modification alters the activity of these proteins and others affecting cell survival under stress conditions and facilitating DNA repair mechanisms showing its comprehensive integration in these important cellular processes.

Alterations in Sumo 2 function have links to neurodegenerative diseases and cancer. Abnormal SUMOylation patterns involving Sumo 2 correlate with the progression of Alzheimer's disease and various types of tumors. In these contexts Sumo 2 modulates proteins such as tau and MDM2 which are critical in disease development and progression. Exploring the role of Sumo 2 in these conditions highlights its potential as a target for therapeutic intervention and diagnostic applications.
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Specifications

Form

Liquid

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General info

Function

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed : 26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed : 18408734, PubMed : 18538659, PubMed : 21965678, PubMed : 9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed : 24105744).

Sequence similarities

Belongs to the ubiquitin family. SUMO subfamily.

Post-translational modifications

Polymeric chains can be formed through Lys-11 cross-linking. Polymeric SUMO2 chains undergo 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4.. Cleavage of precursor form by SENP1 or SENP2 is necessary for function.. Monoubiquitinated N-terminally by UBE2W, which primes it for RNF4-dependent polyubiquitination by the UBE2V1-UBE2N heterodimer.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed : 26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed : 18408734, PubMed : 18538659, PubMed : 21965678, PubMed : 9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed : 24105744).
See full target information SUMO2
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