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Recombinant Human Superoxide Dismutase 3/EC-SOD protein (His tag) is a Human Full Length protein, in the 19 to 240 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human Superoxide Dismutase 3/EC-SOD protein (His tag) (AB236182), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

W T G E D S A E P N S D S A E W I R D M Y A K V T E I W Q E V M Q R R D D D G A L H A A C Q V Q P S A T L D A A Q P R V T G V V L F R Q L A P R A K L D A F F A L E G F P T E P N S S S R A I H V H Q F G D L S Q G C E S T G P H Y N P L A V P H P Q H P G D F G N F A V R D G S L W R Y R A G L A A S L A G P H S I V G R A V V V H A G E D D L G R G G N Q A S V E N G N A G R R L A C C V V G V C G P G L W E R Q A R E H S E R K K R R R E S E C K A A

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.

Alternative names

Recommended products

Recombinant Human Superoxide Dismutase 3/EC-SOD protein (His tag) is a Human Full Length protein, in the 19 to 240 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE

Accession
P08294-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Sequence info

Amino acid sequence

W T G E D S A E P N S D S A E W I R D M Y A K V T E I W Q E V M Q R R D D D G A L H A A C Q V Q P S A T L D A A Q P R V T G V V L F R Q L A P R A K L D A F F A L E G F P T E P N S S S R A I H V H Q F G D L S Q G C E S T G P H Y N P L A V P H P Q H P G D F G N F A V R D G S L W R Y R A G L A A S L A G P H S I V G R A V V V H A G E D D L G R G G N Q A S V E N G N A G R R L A C C V V G V C G P G L W E R Q A R E H S E R K K R R R E S E C K A A

Accession

P08294

Protein length

Full Length

Predicted molecular weight

28.1 kDa

Amino acids

19 to 240

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

General info

Function

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.

Sequence similarities

Belongs to the Cu-Zn superoxide dismutase family.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

Notes

Previously labelled as Superoxide Dismutase 3.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Superoxide Dismutase 3 also known as EC-SOD is an antioxidant enzyme with a molecular weight of approximately 135 kDa. It's an extracellular enzyme that catalyzes the dismutation of superoxide radicals into hydrogen peroxide and molecular oxygen. EC-SOD is found mostly in the extracellular matrix tissues such as lungs blood vessels and heart. Its activity depends on the presence of metal ions copper and zinc leading its classification as a Cu/Zn SOD enzyme. Scientists often study EC-SOD using ELISA assays because of its importance in neutralizing reactive oxygen species.

Biological function summary

The enzyme acts as an important regulator of oxidative stress by reducing levels of superoxide radicals. EC-SOD forms a complex with heparan sulfate proteoglycans in the extracellular matrix which influences its stability and distribution. This enzyme plays a role in maintaining the redox balance influencing cell signaling inflammation and tissue damage which makes it important in managing oxidative stress in different tissues including the cardiovascular and respiratory systems.

Pathways

Superoxide dismutase 3 participates in antioxidant pathways important for cellular defense against oxidative stress. It is particularly important in the oxidative stress response pathway. By reducing superoxide radicals EC-SOD influences pathways involving other antioxidant proteins like catalase and glutathione peroxidase. These interactions help prevent the accumulation of harmful oxidants and maintain cellular homeostasis. EC-SOD's action connects closely with other superoxide dismutases such as Cu/Zn SOD and MnSOD within these protective pathways.

Associated diseases and disorders

Enhanced or impaired EC-SOD function associates with various health issues such as cardiovascular diseases and chronic obstructive pulmonary disease (COPD). EC-SOD has been shown to protect vascular health by reducing oxidative stress related damages which can mitigate the risk of atherosclerosis. In the context of respiratory conditions like COPD alterations in EC-SOD expression can disrupt lung tissue function and inflammatory responses. Its interactions with proteins like catalase further contribute to its protective role against cell and tissue damage during disease progression.

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1 product image

  • SDS-PAGE - Recombinant Human Superoxide Dismutase 3/EC-SOD protein (His tag) (ab236182), expandable thumbnail

    SDS-PAGE - Recombinant Human Superoxide Dismutase 3/EC-SOD protein (His tag) (ab236182)

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab236182.

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