Recombinant Human Surfactant protein D/SP-D (His tag) is a Human Full Length protein, in the 22 to 375 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A D P E M K T Y S H R T M P S A C T L V M C S S V E S G L P G R D G R D G R E G P R G E K G D P G L P G A A G Q A G M P G Q A G P V G P K G D N G S V G E P G P K G D T G P S G P P G P P G V P G P A G R E G P L G K Q G N I G P Q G K P G P K G E A G P K G E V G A P G M Q G S A G A R G L A G P K G E R G V P G E R G V P G N T G A A G S A G A M G P Q G S P G A R G P P G L K G D K G I P G D K G A K G E S G L P D V A S L R Q Q V E A L Q G Q V Q H L Q A A F S Q Y K K V E L F P N G Q S V G E K I F K T A G F V K P F T E A Q L L C T Q A G G Q L A S P R S A A E N A A L Q Q L V V A K N E A A F L S M T D S K T E G K F T Y P T G E S L V Y S N W A P G E P N D D G G S E D C V E I F T N G K W N D R A C G E K R L V V C E F H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Alternative names
COLEC7, PSPD, SFTP4, SFTPD, Pulmonary surfactant-associated protein D, PSP-D, SP-D, Collectin-7, Lung surfactant protein D
Recommended products
Recombinant Human Surfactant protein D/SP-D (His tag) is a Human Full Length protein, in the 22 to 375 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P35247-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A D P E M K T Y S H R T M P S A C T L V M C S S V E S G L P G R D G R D G R E G P R G E K G D P G L P G A A G Q A G M P G Q A G P V G P K G D N G S V G E P G P K G D T G P S G P P G P P G V P G P A G R E G P L G K Q G N I G P Q G K P G P K G E A G P K G E V G A P G M Q G S A G A R G L A G P K G E R G V P G E R G V P G N T G A A G S A G A M G P Q G S P G A R G P P G L K G D K G I P G D K G A K G E S G L P D V A S L R Q Q V E A L Q G Q V Q H L Q A A F S Q Y K K V E L F P N G Q S V G E K I F K T A G F V K P F T E A Q L L C T Q A G G Q L A S P R S A A E N A A L Q Q L V V A K N E A A F L S M T D S K T E G K F T Y P T G E S L V Y S N W A P G E P N D D G G S E D C V E I F T N G K W N D R A C G E K R L V V C E F H H H H H H
- Accession
- P35247
- Protein length
- Full Length
- Predicted molecular weight
- 36.5 kDa
- Amino acids
- 22 to 375
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
- Sequence similarities
Belongs to the SFTPD family.
- Post-translational modifications
The N-terminus is blocked.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as Surfactant protein D.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Surfactant protein D (SP-D) also known as protein D functions as an important component of the innate immune system. It weighs around 43 kDa and is primarily expressed in pulmonary alveoli where lung cells synthesize it. SP-D belongs to the collectin family and has a C-type lectin domain that allows binding to carbohydrate structures on microbial surfaces. This ability enables SP-D to interact directly with various pathogens and infected cells targeting them for clearance. Researchers often study SP-D with 'anti-surfactant' antibodies using methods like the ELISA protocol including versions like ELISA Sp and ELISA Denise.
- Biological function summary
SP-D contributes to host defense by enhancing the uptake and elimination of pathogens by immune cells such as macrophages. Part of a larger protein complex SP-D can opsonize bacteria and viruses facilitating phagocytosis. Its role extends to modulating inflammation by affecting the production of pro-inflammatory cytokines. By maintaining homeostasis in surfactant composition it indirectly supports lung function and protects against lung injury. SP-D’s function involves communication and transmission of signals within the immune system making it integral to pulmonary immune processes.
- Pathways
Surfactant protein D is involved in the regulation of the complement and coagulation cascades as well as the pathogen recognition pathway. It is closely related to other collectins such as surfactant protein A (SP-A) which share similar mechanisms in the immune response. SP-D's interaction within these pathways highlights its role in identifying and neutralizing pathogens. Through its connections with related proteins SP-D plays an important part in modulating immune responses ensuring effective pathogen elimination without excessive inflammation.
- Associated diseases and disorders
SP-D has been linked with respiratory diseases such as chronic obstructive pulmonary disease (COPD) and pulmonary fibrosis. These conditions may involve impaired SP-D function or expression levels potentially due to genetic factors or environmental influences. SP-D also interacts with proteins like SP-A in these diseases participating in lung immune responses. Disrupted regulation of SP-D can contribute to chronic inflammatory states in the lungs exacerbating disease progression and leading to compromised lung function. Understanding SP-D's role in these contexts offers potential therapeutic insights for targeting pathological inflammation.
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1 product image
SDS-PAGE - Recombinant Human Surfactant protein D/SP-D (His tag) (ab229373)
15% SDS-PAGE analysis of 3 μg ab229373.
MW: 40-57 kDa (SDS-PAGE under reducing conditions).
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