Recombinant Human Survivin protein is a Human Full Length protein, in the 1 to 142 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, sELISA.
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- Nature protocols 16:3522-35462021Probing low-copy-number proteins in single living cells using single-cell plasmonic immunosandwich assays.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Liu,Hui He,Dan Xie,Yanrong Wen,Zhen LiuPubMed 34089021
- Oncotarget 8:114722-1147352017Circulating CD9+/GFAP+/survivin+ exosomes in malignant glioma patients following survivin vaccination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhillip M Galbo et. al.PubMed 29383115
- Molecular therapy : the journal of the American Society of Gene Therapy 23:202-142014First-in-man study of western reserve strain oncolytic vaccinia virus: safety, systemic spread, and antitumor activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHerbert J Zeh,Stephanie Downs-Canner,J Andrea McCart,Zong Sheng Guo,Uma N M Rao,Lekshmi Ramalingam,Stephen H Thorne,Heather L Jones,Pawel Kalinski,Eva Wieckowski,Mark E O'Malley,Manijeh Daneshmand,Kang Hu,John C Bell,Tae-Ho Hwang,Anne Moon,Caroline J Breitbach,David H Kirn,David L BartlettPubMed 25292189
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Calmodulin tag N-Terminus
- Applications
- SDS-PAGE, sELISA
- Biologically active
- No
Amino acid sequence
M G A P T L P P A W Q P F L K D H R I S T F K N W P F L E G C A C T P E R M A E A G F I H C P T E N E P D L A Q C F F C F K E L E G W E P D D D P I E E H K K H S S G C A F L S V K K Q F E E L T L G E F L K L D R E R A K N K I A K E T N N K K K E F E E T A K K V R R A I E Q L A A M D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:20627126, PubMed:21364656, PubMed:25778398, PubMed:28218735, PubMed:9859993). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Essential for the maintenance of mitochondrial integrity and function (PubMed:25778398). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).
Alternative names
API4, IAP4, BIRC5, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4, Apoptosis inhibitor survivin
Recommended products
Recombinant Human Survivin protein is a Human Full Length protein, in the 1 to 142 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, sELISA.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Calmodulin tag N-Terminus
- Applications
- SDS-PAGE, sELISA
- Biologically active
- No
- Accession
- O15392-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 0.58% Sodium chloride, 0.242% Tris
Sequence info
Amino acid sequence
- M G A P T L P P A W Q P F L K D H R I S T F K N W P F L E G C A C T P E R M A E A G F I H C P T E N E P D L A Q C F F C F K E L E G W E P D D D P I E E H K K H S S G C A F L S V K K Q F E E L T L G E F L K L D R E R A K N K I A K E T N N K K K E F E E T A K K V R R A I E Q L A A M D
- Accession
- O15392
- Protein length
- Full Length
- Predicted molecular weight
- 33 kDa
- Amino acids
- 1 to 142
- Nature
- Recombinant
- Tags
- Calmodulin tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab87202 is purified using conventional chromatography techniques.
General info
- Function
Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:20627126, PubMed:21364656, PubMed:25778398, PubMed:28218735, PubMed:9859993). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Essential for the maintenance of mitochondrial integrity and function (PubMed:25778398). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).
- Sequence similarities
Belongs to the IAP family.
- Post-translational modifications
Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting. Deubiquitinated by USP35 or USP38; leading to stabilization (PubMed:34438346).
- Subcellular localisation
- Nucleus, Cytoskeleton, Spindle
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Survivin also known as 'BIRC5 protein' is a small protein with a molecular weight of approximately 16.5 kDa. It belongs to the Inhibitor of Apoptosis (IAP) family carrying out its functions through inhibiting caspases and averting apoptosis. Survivin is expressed highly in embryonic tissues and various cancers but its expression in adult differentiated tissues is low. This selective expression pattern makes Survivin an attractive target for cancer research and therapeutic development.
- Biological function summary
Survivin aids cell division and regulates apoptosis. It plays a major role as a chromosomal passenger protein functioning in concert with other members of the chromosomal passenger complex including Aurora B kinase INCENP and Borealin. During mitosis Survivin helps ensure proper chromosome alignment segregation and cytokinesis. Its ability to inhibit apoptosis allows it to enhance cell survival contributing to tumor progression when deregulated.
- Pathways
Survivin is part of the cell cycle regulation and apoptosis pathways. It plays an integral role in the mitotic spindle checkpoint working closely with Aurora B kinase to regulate mitosis. Additionally the protein interfaces with the extrinsic apoptosis pathway interacting with members like XIAP to prevent caspase activation and cell death. These interactions reveal Survivin's dual function in promoting mitosis and regulating apoptosis which is essential for maintaining cellular homeostasis.
- Associated diseases and disorders
Survivin is highly connected to cancer and neurodegenerative disorders. Its overexpression links with cancers such as colorectal cancer where it promotes malignancy through inhibiting apoptosis and ensuring unchecked cell division. In neurodegenerative disorders abnormal regulation of Survivin could lead to insufficient cellular survival contributing to diseases like Alzheimer's. The relationship between Survivin and XIAP in cancer highlights its potential as a therapeutic target aiming to induce apoptosis in cancer cells by overcoming Survivin-mediated resistance.
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2 product images
Sandwich ELISA - Recombinant Human Survivin protein (ab87202)
Standard curve for Survivin (Analyte: ab87202); dilution range 1pg/ml to 1μg/ml using Capture Antibody ab27468 at 1μg/ml and Detector Antibody Rabbit monoclonal [EP2880Y] to Survivin (Anti-Survivin antibody [EP2880Y] ab76424) at 0.5μg/ml. Concentration of Rabbit monoclonal [EP2880Y] to Survivin (Anti-Survivin antibody [EP2880Y] ab76424) may vary from lot to lot; please use this curve as guideline.
SDS-PAGE - Recombinant Human Survivin protein (ab87202)
15% SDS-PAGE showing ab87202 at approximately 33kDa (3μg).
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