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AB140585

Recombinant Human Synaptotagmin 4 protein

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Recombinant Human Synaptotagmin 4 protein is a Human Fragment protein, in the 38 to 425 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

KIAA1342, SYT4, Synaptotagmin-4, Synaptotagmin IV, SytIV

1 Images
SDS-PAGE - Recombinant Human Synaptotagmin 4 protein (AB140585)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Synaptotagmin 4 protein (AB140585)

15% SDS-PAGE analysis of ab140585 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9H2B2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMCQRKSSKSNKTPPYKFVHVLKGVDIYPENLNSKKKFGADDKNEVKNKPAVPKNSLHLDLEKRDLNGNFPKTNLKPGSPSDLENATPKLFLEGEKESVSPESLKSSTSLTSEEKQEKLGTLFFSLEYNFERKAFVVNIKEARGLPAMDEQSMTSDPYIKMTILPEKKHKVKTRVLRKTLDPAFDETFTFYGIPYTQIQELALHFTILSFDRFSRDDIIGEVLIPLSGIELSEGKMLMNREIIKRNVRKSSGRGELLISLCYQSTTNTLTVVVLKARHLPKSDVSGLSDPYVKVNLYHAKKRISKKKTHVKKCTPNAVFNELFVFDIPCEGLEDISVEFLVLDSERGSRNEVIGQLVLGAAAEGTGGEHWKEICDYPRRQIAKWHVLCDG","proteinLength":"Fragment","predictedMolecularWeight":"46.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":425,"aminoAcidStart":38,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H2B2","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synaptotagmin 4 also known as SYT4 is a calcium-dependent protein involved in the regulation of synaptic vesicle exocytosis. It belongs to the synaptotagmin family and exhibits a molecular weight of approximately 47 kDa. This protein primarily localizes in the brain where it is associated with the synaptic membrane. Synaptotagmin 4 features two C2 domains C2A and C2B which enable it to bind to phospholipids in a calcium-dependent manner suggesting its important role in synaptic transmission and signal modulation.
Biological function summary

Synaptotagmin 4 modulates neurotransmitter release at synapses by acting as a calcium sensor. It does not directly trigger vesicle fusion but appears to regulate the process fine-tuning synaptic plasticity and strength. It functions as part of a complex that includes other synaptotagmins and participates in the regulation of neuronal communication and plasticity. Additionally this protein influences learning and memory processes by affecting the short-term dynamics of neurotransmitter release.

Pathways

Synaptotagmin 4 participates in the synaptic vesicle cycle where it influences the docking and priming of synaptic vesicles before fusion. It plays a role in the calcium signaling pathway essential for neurotransmitter release. Within these pathways SYT4 interacts with proteins such as SNAP-25 and syntaxin which are critical for vesicle fusion and neurotransmission. Its regulatory capacity helps maintain synaptic efficacy and contributes to the adaptability of neural networks.

Synaptotagmin 4 has been associated with neurological conditions such as autism spectrum disorders and schizophrenia. Alterations in the expression or function of this protein can lead to impaired synaptic communication which is a characteristic observed in these disorders. During the pathological conditions SYT4 may interact with other proteins like neurexins and neuroligins which are also implicated in synaptic dysfunction present in these disorders. Understanding SYT4's function and interactions could provide insights into potential therapeutic strategies.
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Specifications

Form

Liquid

Additional notes

ab140585 is purified using conventional chromatography techniques.

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General info

Function

Synaptotagmin family member which does not bind Ca(2+) (By similarity) (PubMed : 23999003). Involved in neuronal dense core vesicles (DCVs) mobility through its interaction with KIF1A. Upon increased neuronal activity, phosphorylation by MAPK8/JNK1 destabilizes the interaction with KIF1A and captures DCVs to synapses (By similarity). Plays a role in dendrite formation by melanocytes (PubMed : 23999003).

Sequence similarities

Belongs to the synaptotagmin family.

Post-translational modifications

Phosphorylation at Ser-135 by MAPK8/JNK1 reduces interaction with KIF1A and neuronal dense core vesicles mobility.

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Product protocols

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Target data

Synaptotagmin family member which does not bind Ca(2+) (By similarity) (PubMed : 23999003). Involved in neuronal dense core vesicles (DCVs) mobility through its interaction with KIF1A. Upon increased neuronal activity, phosphorylation by MAPK8/JNK1 destabilizes the interaction with KIF1A and captures DCVs to synapses (By similarity). Plays a role in dendrite formation by melanocytes (PubMed : 23999003).
See full target information SYT4
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