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AB171492

Recombinant Human Synaptotagmin V protein (His tag N-Terminus)

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Recombinant Human Synaptotagmin V protein (His tag N-Terminus) is a Human Fragment protein, in the 109 to 386 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Synaptotagmin-5, Synaptotagmin V, SytV, SYT5

1 Images
SDS-PAGE - Recombinant Human Synaptotagmin V protein (His tag N-Terminus) (AB171492)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Synaptotagmin V protein (His tag N-Terminus) (AB171492)

15% SDS-PAGE analysis of 3µg ab171492.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O00445

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSLGRLQYSLDYDFQSGQLLVGILQAMGLAALDLGGSSDPYVRVYLLPDKRRRYETKVHRQTLNPHFGETFAFKVPYVELGGRVLVMAVYDFDRFSRNDAIGEVRVPMSSVDLGRPVQAWRELQAAPREEQEKLGDICFSLRYVPTAGKLTVIVLEAKNLKKMDVGGLSDPYVKVHLLQGGKKVRKKKTTIKKNTLNPYYNEAFSFEVPCDQVQKVQVELTVLDYDKLGKNEAIGRVAVGAAAGGAGLRHWADMLANPRRPIAQWHSLRPPDRVRLLPAP","proteinLength":"Fragment","predictedMolecularWeight":"33.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":386,"aminoAcidStart":109,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O00445","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synaptotagmin V also known as SYT5 is a member of the synaptotagmin family of proteins. These proteins act as calcium sensors that regulate synaptic vesicle exocytosis in neurons. Synaptotagmin V has a molecular mass of approximately 47 kDa and contains a transmembrane domain which allows it to anchor to vesicle membranes. It expresses widely in neuronal tissues contributing to neurotransmitter release by responding to calcium influx.
Biological function summary

Synaptotagmin V participates in the synaptic vesicle cycle specifically in synaptic vesicle docking and fusion processes at the presynaptic membrane. It does not work alone but rather as part of a larger protein complex involving SNARE proteins. These interactions allow for precise regulation and timing of neurotransmitter release which is essential for proper synaptic transmission.

Pathways

Synaptotagmin V significantly contributes to neurotransmitter release by mediating calcium-dependent membrane fusion in synaptic vesicle cycling. It plays a critical role within the calcium signaling pathway affecting how neurons communicate. Related proteins such as SNAP25 and syntaxin-1 facilitate its function in this pathway interacting with synaptotagmin V to ensure efficient synaptic transmission.

Synaptotagmin V has connections with neurodegenerative conditions like Alzheimer's disease and Huntington's disease. Altered synaptotagmin V expression or function can impact synaptic transmission potentially contributing to these disorders by disrupting normal neuronal communication. The protein links to amyloid precursor protein (APP) and tau both associated with Alzheimer's disease through its role in vesicle fusion and neurotransmitter release.
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Specifications

Form

Liquid

Additional notes

ab171492 was purified using conventional chromatography techniques.

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General info

Function

May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface (By similarity).

Sequence similarities

Belongs to the synaptotagmin family.

Subcellular localisation

Recycling endosome membrane

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Product protocols

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Target data

May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface (By similarity).
See full target information Synaptotagmin-5
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