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AB131680

Recombinant Human Syntaxin 4 protein (His tag N-Terminus)

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Recombinant Human Syntaxin 4 protein (His tag N-Terminus) is a Human Fragment protein, in the 1 to 275 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

STX4A, STX4, Syntaxin-4, Renal carcinoma antigen NY-REN-31

1 Images
SDS-PAGE - Recombinant Human Syntaxin 4 protein (His tag N-Terminus) (AB131680)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Syntaxin 4 protein (His tag N-Terminus) (AB131680)

15% SDS-PAGE analysis of 3 µg ab131680.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q12846

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMMRDRTHELRQGDDSSDEEDKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIRELHDIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKTALENQKKARKKKVL","proteinLength":"Fragment","predictedMolecularWeight":"34.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":275,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q12846","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 4 also known as STX4 is a member of the soluble NSF attachment protein receptor (SNARE) family. It has a molecular mass of approximately 33 kDa. Syntaxin 4 is integral to membrane fusion processes specifically docking and fusion of vesicles with target membranes. It expresses in a wide range of tissues but high levels are found in skeletal muscle and adipose tissue. Its function is necessary in cellular trafficking systems and exocytosis.
Biological function summary

Syntaxin 4 facilitates key transport events at the plasma membrane. It forms part of the SNARE complex which is essential for vesicle targeting and fusion. This complex includes other SNARE proteins such as VAMP2 and SNAP23 ensuring that vesicles deliver their content accurately within cells. This function is critical in processes such as insulin-stimulated glucose uptake and neurotransmitter release.

Pathways

Syntaxin 4 involves itself in the regulation of vesicular transport and insulin signaling. It plays an essential role in the GLUT4 translocation pathway where Syntaxin 4 interacts with proteins like Munc18c which is critical for the regulation of glucose transport in response to insulin. Another important pathway includes its involvement in neurotransmitter release where Syntaxin 4 interfaces with synaptotagmin highlighting its influence within signal transmission processes.

Dysfunctions related to Syntaxin 4 have implications in conditions such as type 2 diabetes and certain neuropathies. In the context of type 2 diabetes impaired Syntaxin 4-mediated uptake of glucose into cells can lead to insulin resistance. Syntaxin 4 also relates to neuromuscular disorders where its malfunction can alter neurotransmitter release. These conditions oftentimes involve Munc18c which stabilizes Syntaxin 4 and modulates its activity within the cell.
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Specifications

Form

Liquid

Additional notes

ab131680 was purified using conventional chromatography techniques.

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General info

Function

Plasma membrane t-SNARE that mediates docking of transport vesicles (By similarity). Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane (By similarity). In neurons, recruited at neurite tips to membrane domains rich in the phospholipid 1-oleoyl-2-palmitoyl-PC (OPPC) which promotes neurite tip surface expression of the dopamine transporter SLC6A3/DAT by facilitating fusion of SLC6A3-containing transport vesicles with the plasma membrane (By similarity). Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones (By similarity). Required for normal hearing (PubMed : 36355422).

Sequence similarities

Belongs to the syntaxin family.

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Product protocols

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Target data

Plasma membrane t-SNARE that mediates docking of transport vesicles (By similarity). Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane (By similarity). In neurons, recruited at neurite tips to membrane domains rich in the phospholipid 1-oleoyl-2-palmitoyl-PC (OPPC) which promotes neurite tip surface expression of the dopamine transporter SLC6A3/DAT by facilitating fusion of SLC6A3-containing transport vesicles with the plasma membrane (By similarity). Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones (By similarity). Required for normal hearing (PubMed : 36355422).
See full target information STX4
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Complementary Products

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AB184545

Anti-Syntaxin 4 antibody [EPR15473]

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Western blot - Anti-Syntaxin 4 antibody [EPR15473] (AB184545)

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Primary Antibodies

AB88056

Anti-Glucokinase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Glucokinase antibody (AB88056)

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Primary Antibodies

AB133673

Anti-Alpha SNAP antibody [EPR7372]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] (AB133673)

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Primary Antibodies

AB272736

Anti-Syntaxin 1a antibody [EPR23457-15]

BOND RX™ Validated|RabMAb|Recombinant

Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR23457-15] (AB272736)

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