Recombinant Human TADA3L protein is a Human Full Length protein, in the 1 to 432 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S E L K D C P L Q F H D F K S V D H L K V C P R Y T A V L A R S E D D G I G I E E L D T L Q L E L E T L L S S A S R R L R V L E A E T Q I L T D W Q D K K G D R R F L K L G R D H E L G A P P K H G K P K K Q K L E G K A G H G P G P G P G R P K S K N L Q P K I Q E Y E F T D D P I D V P R I P K N D A P N R F W A S V E P Y C A D I T S E E V R T L E E L L K P P E D E A E H Y K I P P L G K H Y S Q R W A Q E D L L E E Q K D G A R A A A V A D K K K G L M G P L T E L D T K D V D A L L K K S E A Q H E Q P E D G C P F G A L T Q R L L Q A L V E E N I I S P M E D S P I P D M S G K E S G A D G A S T S P R N Q N K P F S V P H T K S L E S R I K E E L I A Q G L L E S E D R P A E D S E D E V L A E L R K R Q A E L K A L S A H N R T K K H D L L R L A K E E V S R Q E L R Q R V R M A D N E V M D A F R K I M A A R Q K K R T P T K K E K D Q A W K T L K E R E S I L K L L D G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Also known as a coactivator for p53/TP53-dependent transcriptional activation. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.
Alternative names
ADA3, TADA3L, TADA3, Transcriptional adapter 3, ADA3 homolog, STAF54, Transcriptional adapter 3-like, hADA3, ADA3-like protein
Recommended products
Recombinant Human TADA3L protein is a Human Full Length protein, in the 1 to 432 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O75528-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S E L K D C P L Q F H D F K S V D H L K V C P R Y T A V L A R S E D D G I G I E E L D T L Q L E L E T L L S S A S R R L R V L E A E T Q I L T D W Q D K K G D R R F L K L G R D H E L G A P P K H G K P K K Q K L E G K A G H G P G P G P G R P K S K N L Q P K I Q E Y E F T D D P I D V P R I P K N D A P N R F W A S V E P Y C A D I T S E E V R T L E E L L K P P E D E A E H Y K I P P L G K H Y S Q R W A Q E D L L E E Q K D G A R A A A V A D K K K G L M G P L T E L D T K D V D A L L K K S E A Q H E Q P E D G C P F G A L T Q R L L Q A L V E E N I I S P M E D S P I P D M S G K E S G A D G A S T S P R N Q N K P F S V P H T K S L E S R I K E E L I A Q G L L E S E D R P A E D S E D E V L A E L R K R Q A E L K A L S A H N R T K K H D L L R L A K E E V S R Q E L R Q R V R M A D N E V M D A F R K I M A A R Q K K R T P T K K E K D Q A W K T L K E R E S I L K L L D G
- Accession
- O75528
- Protein length
- Full Length
- Predicted molecular weight
- 51.3 kDa
- Amino acids
- 1 to 432
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab171703 is purified using conventional chromatography techniques.
General info
- Function
Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Also known as a coactivator for p53/TP53-dependent transcriptional activation. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.
- Sequence similarities
Belongs to the NGG1 family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TADA3L also known as Transcriptional Adaptor 3-Like is part of the larger transcriptional coactivator complexes. The protein has a mass of approximately 55 kDa and is expressed in various tissues including liver and kidney. Mechanically TADA3L acts as a transcriptional adaptor interacting with DNA-binding transcription factors and assisting the recruitment of additional coactivators necessary for gene transcription.
- Biological function summary
The protein assists in regulating gene expression by serving as a component of the human Ada-Two-A containing (hATAC) complex. The hATAC complex which includes TADA3L plays a role in histone acetylation allowing chromatin to be more accessible for transcriptional machinery. TADA3L's involvement in this complex underlines its role in modulating transcription and influencing cell cycle and proliferation processes.
- Pathways
The TADA3L protein integrates into networks influencing transcriptional regulation and chromatin remodeling pathways. It associates with GCN5 and PCAF which are critical for histone acetyl transferase (HAT) activity within these pathways. Through its interaction with these proteins TADA3L contributes to the control of gene expression and is an important part of maintaining homeostasis in cellular function.
- Associated diseases and disorders
Alterations in TADA3L function or expression link to cancer and developmental disorders. Mutations or deregulation of this protein can disrupt normal transcriptional regulation leading to oncogenesis. In cancer pathways TADA3L shows connections with proteins like p53 a widely studied tumor suppressor where its impairment could affect apoptotic and cell cycle arrest pathways contributing to tumor development.
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1 product image
SDS-PAGE - Recombinant Human TADA3L protein (ab171703)
15% SDS-PAGE analysis of ab171703 (3μg).
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