Recombinant Human TAF1 protein is a Human Fragment protein, in the 1400 to 1518 aa range, expressed in Escherichia coli, with >=95% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >=95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M H H H H H H T D P M V T L S S I L E S I I N D M R D L P N T Y P F H T P V N A K V V K D Y Y K I I T R P M D L Q T L R E N V R K R L Y P S R E E F R E H L E L I V K N S A T Y N G P K H S L T Q I S Q S M L D L C D E K L K E K E D K L A R L E K A I N P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473). TAF1 is the largest component and core scaffold of the TFIID complex, involved in nucleating complex assembly (PubMed:25412659, PubMed:27007846, PubMed:33795473). TAF1 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF2 (PubMed:33795473). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors (PubMed:25412659, PubMed:8625415). Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53 (PubMed:25412659). Phosphorylates GTF2A1 and GTF2F1 on Ser residues (PubMed:25412659). Possesses DNA-binding activity (PubMed:25412659). Essential for progression of the G1 phase of the cell cycle (PubMed:11278496, PubMed:15053879, PubMed:2038334, PubMed:8450888, PubMed:8625415, PubMed:9660973, PubMed:9858607). Exhibits histone acetyltransferase activity towards histones H3 and H4 (PubMed:15870300).
Alternative names
BA2R, CCG1, CCGS, TAF2A, TAF1, Transcription initiation factor TFIID subunit 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa, Transcription initiation factor TFIID 250 kDa subunit, p250, TAF(II)250, TAFII-250, TAFII250
Recommended products
Recombinant Human TAF1 protein is a Human Fragment protein, in the 1400 to 1518 aa range, expressed in Escherichia coli, with >=95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P21675-2
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Tris HCl, 0.63% Sodium chloride, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M H H H H H H T D P M V T L S S I L E S I I N D M R D L P N T Y P F H T P V N A K V V K D Y Y K I I T R P M D L Q T L R E N V R K R L Y P S R E E F R E H L E L I V K N S A T Y N G P K H S L T Q I S Q S M L D L C D E K L K E K E D K L A R L E K A I N P
- Accession
- P21675
- Protein length
- Fragment
- Predicted molecular weight
- 14.9 kDa
- Amino acids
- 1400 to 1518
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473). TAF1 is the largest component and core scaffold of the TFIID complex, involved in nucleating complex assembly (PubMed:25412659, PubMed:27007846, PubMed:33795473). TAF1 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF2 (PubMed:33795473). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors (PubMed:25412659, PubMed:8625415). Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53 (PubMed:25412659). Phosphorylates GTF2A1 and GTF2F1 on Ser residues (PubMed:25412659). Possesses DNA-binding activity (PubMed:25412659). Essential for progression of the G1 phase of the cell cycle (PubMed:11278496, PubMed:15053879, PubMed:2038334, PubMed:8450888, PubMed:8625415, PubMed:9660973, PubMed:9858607). Exhibits histone acetyltransferase activity towards histones H3 and H4 (PubMed:15870300).
- Sequence similarities
Belongs to the TAF1 family.
- Post-translational modifications
Phosphorylated by casein kinase II in vitro.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Notes
ab198086 is useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TAF1 also known as TATA-box binding protein associated factor 1 has a mechanical role as a transcription factor in the initiation process of transcription. This protein forms a part of the larger transcription factor IID (TFIID) complex weighing approximately 200 kDa. TAF1 expresses broadly in tissues where it plays an important role in mediating transcriptional regulation. It is a general transcription factor that serves as a molecular scaffold essential for recruiting the necessary machinery to specific promotor regions of DNA for transcription activation.
- Biological function summary
TAF1 interacts extensively with other TFIID components to control the formation of the pre-initiation complex on core promoters. This protein is integral to the regulation of gene expression by modulating transcriptional activity in cells. TAF1 acts as a histone acetyltransferase and a kinase modifying other proteins' functions and structural capabilities in the transcription process. Its enzymatic activities make it essential for the phosphorylation and acetylation of histones directly impacting the accessibility of chromatin for transcription.
- Pathways
TAF1 functions prominently in the RNA polymerase II transcription initiation pathway. Its interactions with TATA-binding protein and other TAFs facilitate the robust expression of genes essential for various cellular functions. TAF1 establishes connections with proteins like TBP acting within the canonical pathway that guides precise transcription initiation. Additionally TAF1 partakes in the p53 signaling pathway where it interacts with several p53 regulators influencing cell cycle regulation and apoptosis.
- Associated diseases and disorders
TAF1 mutations are linked to X-linked dystonia-parkinsonism and certain neurodegenerative disorders. Aberrations in TAF1 function impact transcriptional regulation leading to dysregulation in neuronal gene expression. Furthermore TAF1 associations with Huntington's disease have been documented highlighting its role in neuronal survival and stress response. In these diseases altered pathways implicate TAF1 with other proteins such as Huntingtin underlining its importance in maintaining neuronal health and function.
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1 product image
SDS-PAGE - Recombinant Human TAF1 protein (ab198086)
4-20% SDS PAGE analysis of ab198086 (1.9 μg).
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