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Recombinant human TAF1 protein is a Human Fragment protein, in the 1400 to 1651 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
Alternative names=Transcription initiation factor TFIID subunit 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa, Transcription initiation factor TFIID 250 kDa subunit, p250, TAF(II)250, TAFII-250, TAFII250, TAF2A, TAF1, BA2R, CCG1, CCGS
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Sequence
T D P M V T L S S I L E S I I N D M R D L P N T Y P F H T P V N A K V V K D Y Y K I I T R P M D L Q T L R E N V R K R L Y P S R E E F R E H L E L I V K N S A T Y N G P K H S L T Q I S Q S M L D L C D E K L K E K E D K L A R L E K A I N P L L D D D D Q V A F S F I L D N I V T Q K M M A V P D S W P F H H P V N K K F V P D Y Y K V I V N P M D L E T I R K N I S K H K Y Q S R E S F L D D V N L I L A N S V K Y N G P E S Q Y T K T A Q E I V N V C Y Q T L T E Y D E H L T Q L E K D I C T A K E A A L E E A E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human TAF1 protein is a Human Fragment protein, in the 1400 to 1651 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
Alternative names=Transcription initiation factor TFIID subunit 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa, Transcription initiation factor TFIID 250 kDa subunit, p250, TAF(II)250, TAFII-250, TAFII250, TAF2A, TAF1, BA2R, CCG1, CCGS
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
ab198139 is incubated with biotinylated acetyl-histone peptide substrate in TAF1 assay buffer in a 10 μl reaction for 1 hour at RT. GSH acceptor beads were then added, and the mixture was incubated for 30 minutes at room temperature (RT). Streptavidin-conjugated donor beads were then added. After 10 minute incubation at RT, Alphacounts are measured.
- Predicted molecular weight
56.1 kDa
- Amino acids
1400 to 1651
- Sequence
T D P M V T L S S I L E S I I N D M R D L P N T Y P F H T P V N A K V V K D Y Y K I I T R P M D L Q T L R E N V R K R L Y P S R E E F R E H L E L I V K N S A T Y N G P K H S L T Q I S Q S M L D L C D E K L K E K E D K L A R L E K A I N P L L D D D D Q V A F S F I L D N I V T Q K M M A V P D S W P F H H P V N K K F V P D Y Y K V I V N P M D L E T I R K N I S K H K Y Q S R E S F L D D V N L I L A N S V K Y N G P E S Q Y T K T A Q E I V N V C Y Q T L T E Y D E H L T Q L E K D I C T A K E A A L E E A E
- Accession
- P21675-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride
Specifications
- Form
Liquid
General info
- Function
Largest component and core scaffold of the TFIID basal transcription factor complex (PubMed:25412659, PubMed:27007846). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity (PubMed:25412659). Essential for progression of the G1 phase of the cell cycle (PubMed:11278496, PubMed:15053879, PubMed:2038334, PubMed:8450888, PubMed:8625415, PubMed:9660973, PubMed:9858607). Exhibits histone acetyltransferase activity towards histones H3 and H4 (PubMed:15870300).
- Sequence similarities
Belongs to the TAF1 family.
- Post-translational modifications
Phosphorylated by casein kinase II in vitro.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
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