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AB171687

Recombinant Human TAF10 protein (His tag N-Terminus)

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Recombinant Human TAF10 protein (His tag N-Terminus) is a Human Fragment protein, in the 84 to 218 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

TAF2A, TAF2H, TAFII30, TAF10, Transcription initiation factor TFIID subunit 10, STAF28, Transcription initiation factor TFIID 30 kDa subunit, TAF(II)30, TAFII-30

1 Images
SDS-PAGE - Recombinant Human TAF10 protein (His tag N-Terminus) (AB171687)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human TAF10 protein (His tag N-Terminus) (AB171687)

15% SDS-PAGE analysis of ab171687 (3µg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q12962

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSGAAPPEGAISNGVYVLPSAANGDVKPVVSSTPLVDFLMQLEDYTPTIPDAVTGYYLNRAGFEASDPRIIRLISLAAQKFISDIANDALQHCKMKGTASGSSRSKSKDRKYTLTMEDLTPALSEYGINVKKPHYFT","proteinLength":"Fragment","predictedMolecularWeight":"16.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":218,"aminoAcidStart":84,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q12962","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAF10 also known as TATA-box binding protein associated factor 10 is a component of the transcription machinery. It has a molecular weight of approximately 21 kDa. This protein is involved in assembling the general transcription factors necessary for the transcription of class II genes. TAF10 is broadly expressed in many tissues particularly in cells that undergo frequent transcriptional activity such as those in the liver and kidney.
Biological function summary

TAF10 acts as an integral part of the transcription factor IID (TFIID) complex which is essential for RNA polymerase II-driven transcription initiation. TAF10 contributes to the recognition and binding of promoter DNA sequences facilitating transcription initiation. It also serves a regulatory role in mediating interactions between the TFIID complex and other transcription factors enhancing transcriptional control and efficiency.

Pathways

TAF10 plays an important role in the regulation of gene expression and RNA polymerase II transcription pathways. It interacts with various components of the transcription initiation complex to initiate and regulate transcription. TAF10 associates with other TAFs and TBP (TATA-binding protein) in the TFIID complex together forming a scaffold for the correct assembly of RNA polymerase II.

TAF10 has been associated with neurodegenerative disorders and certain cancers. Mutations or dysregulation of TAF10 can lead to disruptions in gene expression contributing to the pathogenesis of these conditions. In the case of cancer TAF10 intersects with tumor suppressor proteins such as p53 influencing cell cycle regulation and tumor growth. These interactions highlight TAF10's importance in maintaining cellular homeostasis and its potential role in disease mechanisms.
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Specifications

Form

Liquid

Additional notes

ab171687 is purified using conventional chromatography techniques.

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General info

Function

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 33795473). TAF10 is also component of the PCAF histone acetylase complex, the TATA-binding protein-free TAF complex (TFTC) and the STAGA transcription coactivator-HAT complex (PubMed : 10373431, PubMed : 11564863, PubMed : 12601814, PubMed : 18206972, PubMed : 9885574). May regulate cyclin E expression (By similarity).

Sequence similarities

Belongs to the TAF10 family.

Post-translational modifications

Monomethylated at Lys-189 by SETD7, leading to increased affinity for RNA polymerase II.. Lysine deamination at Lys-189 to form allysine is mediated by LOXL2. Allysine formation by LOXL2 results in release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription.

Subcellular localisation

Nucleus

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Product protocols

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Target data

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 33795473). TAF10 is also component of the PCAF histone acetylase complex, the TATA-binding protein-free TAF complex (TFTC) and the STAGA transcription coactivator-HAT complex (PubMed : 10373431, PubMed : 11564863, PubMed : 12601814, PubMed : 18206972, PubMed : 9885574). May regulate cyclin E expression (By similarity).
See full target information TAF10
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