Recombinant Human TAF15 protein is a Human Fragment protein, in the 148 to 406 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S S Y H S Q R E N Y S H H T Q D D R R D V S R Y G E D N R G Y G G S Q G G G R G R G G Y D K D G R G P M T G S S G G D R G G F K N F G G H R D Y G P R T D A D S E S D N S D N N T I F V Q G L G E G V S T D Q V G E F F K Q I G I I K T N K K T G K P M I N L Y T D K D T G K P K G E A T V S F D D P P S A K A A I D W F D G K E F H G N I I K V S F A T R R P E F M R G G G S G G G R R G R G G Y R G R G G F Q G R G G D P K S G D W V C P N P S C G N M N F A R R N S C N Q C N E P R P E D S R P S G G D F R G R G Y G G E R G Y R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
Alternative names
RBP56, TAF2N, TAF15, TATA-binding protein-associated factor 2N, 68 kDa TATA-binding protein-associated factor, RNA-binding protein 56, TAF(II)68, TAFII68
Recommended products
Recombinant Human TAF15 protein is a Human Fragment protein, in the 148 to 406 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q92804-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S S Y H S Q R E N Y S H H T Q D D R R D V S R Y G E D N R G Y G G S Q G G G R G R G G Y D K D G R G P M T G S S G G D R G G F K N F G G H R D Y G P R T D A D S E S D N S D N N T I F V Q G L G E G V S T D Q V G E F F K Q I G I I K T N K K T G K P M I N L Y T D K D T G K P K G E A T V S F D D P P S A K A A I D W F D G K E F H G N I I K V S F A T R R P E F M R G G G S G G G R R G R G G Y R G R G G F Q G R G G D P K S G D W V C P N P S C G N M N F A R R N S C N Q C N E P R P E D S R P S G G D F R G R G Y G G E R G Y R
- Accession
- Q92804
- Protein length
- Fragment
- Predicted molecular weight
- 30 kDa
- Amino acids
- 148 to 406
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab174418 is purified using conventional chromatography techniques.
General info
- Function
RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
- Sequence similarities
Belongs to the RRM TET family.
- Post-translational modifications
Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TAF15 also known as TATA-binding protein-associated factor 15 is a RNA polymerase II-associated factor with approximate mass of 56 kDa. It belongs to the FET family of proteins which include FUS and EWS. This protein expresses widely in various tissues including the brain and immune cells. Mechanically TAF15 participates in transcription by mediating interactions between transcription factors and the RNA polymerase II complex.
- Biological function summary
The protein helps regulate transcription initiation and elongation. TAF15 operates as a part of the transcription pre-initiation complex working closely with other transcription-associated factors to control gene expression. Its role extends beyond the nucleus as it may participate in RNA processing and transport due to its ability to bind RNA.
- Pathways
TAF15 plays a role in the regulation of transcription from RNA polymerase II promoters. It interacts with numerous other proteins such as TFIID and RNA polymerase II subunits facilitating transcriptional regulation. TAF15 also associates with pathways related to stress response and cellular growth emphasizing its role in maintaining cellular integrity and function.
- Associated diseases and disorders
TAF15 links to certain forms of cancer and neurodegenerative diseases. Its misregulation or mutation associates with some sarcomas indicating a role in tumorigenesis possibly through interactions with oncogenic proteins like EWS. In neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS) TAF15 might be implicated potentially through pathways shared with proteins like TDP-43 that are involved in RNA metabolism dysfunction.
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1 product image
SDS-PAGE - Recombinant Human TAF15 protein (ab174418)
15% SDS-PAGE analysis of ab174418.
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