Recombinant Human TAGLN/Transgelin protein is a Human Full Length protein, in the 1 to 201 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS, WB.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS, WB
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A N K G P S Y G M S R E V Q S K I E K K Y D E E L E E R L V E W I I V Q C G P D V G R P D R G R L G F Q V W L K N G V I L S K L V N S L Y P D G S K P V K V P E N P P S M V F K Q M E Q V A Q F L K A A E D Y G V I K T D M F Q T V D L F E G K D M A A V Q R T L M A L G S L A V T K N D G H Y R G D P N W F M K K A Q E H K R E F T E S Q L Q E G K H V I G L Q M G S N R G A S Q A G M T G Y G R P R Q I I S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab101469 can be used as a WB positive control in conjunction with Anti-TAGLN/Transgelin antibody ab14106. |
Target data
Function
Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
Alternative names
SM22, WS3-10, TAGLN, Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha
Recommended products
Recombinant Human TAGLN/Transgelin protein is a Human Full Length protein, in the 1 to 201 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS, WB.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS, WB
- Biologically active
- No
- Accession
- Q01995-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A N K G P S Y G M S R E V Q S K I E K K Y D E E L E E R L V E W I I V Q C G P D V G R P D R G R L G F Q V W L K N G V I L S K L V N S L Y P D G S K P V K V P E N P P S M V F K Q M E Q V A Q F L K A A E D Y G V I K T D M F Q T V D L F E G K D M A A V Q R T L M A L G S L A V T K N D G H Y R G D P N W F M K K A Q E H K R E F T E S Q L Q E G K H V I G L Q M G S N R G A S Q A G M T G Y G R P R Q I I S
- Accession
- Q01995
- Protein length
- Full Length
- Predicted molecular weight
- 24.8 kDa
- Amino acids
- 1 to 201
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab101469 was purified using conventional chromatography techniques.
General info
- Function
Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
- Sequence similarities
Belongs to the calponin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as TAGLN
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TAGLN also known as Transgelin or SM22-alpha is a protein with a molecular weight of approximately 22 kDa. Structurally it belongs to the calponin family and contains an ACT domain which is critical for its actin-binding ability. It is expressed abundantly in smooth muscle cells where it stabilizes cytoskeletal dynamics by interacting with actin filaments. TAGLN also shows expression in fibroblasts and myofibroblasts supporting structural integrity and cellular movement.
- Biological function summary
The regulatory involvement of TAGLN in smooth muscle contraction influences cellular activities essential for vascular function. This protein modulates remodeling and differentiation within smooth muscle cells. It can associate with actin and calponin to form complexes that regulate the stability of cytoskeletal structures ensuring effective cellular responses to mechanical stimuli. By linking signal transduction to cytoskeletal mechanics TAGLN facilitates the maintenance of cell shape and tension.
- Pathways
TAGLN plays a role in the regulation of the RhoA/Rho kinase pathway contributing to actin cytoskeleton organization and smooth muscle contraction. Additionally it is involved in the WNT signaling pathway influencing cell proliferation and differentiation. In these pathways TAGLN works with proteins like RhoA and beta-catenin to transmit signals from the extracellular environment to the nucleus impacting gene expression and cellular responses.
- Associated diseases and disorders
Disruptions in TAGLN expression associate with vascular diseases and certain cancers. Downregulation or altered expression patterns can lead to smooth muscle cell dysfunction contributing to atherosclerosis. Similarly TAGLN interacts with proteins such as fibronectin and MMP2 in cancer where its altered expression could promote metastasis and tumor progression. Understanding these connections highlights TAGLN's potential as a target for therapeutic interventions in these diseases.
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2 product images
Western blot - Recombinant Human TAGLN/Transgelin protein (ab101469)
All lanes: Western blot - Anti-TAGLN/Transgelin antibody (Anti-TAGLN/Transgelin antibody ab14106) at 1 µg/mL
Lane 1: Western blot - Recombinant Human TAGLN/Transgelin protein (ab101469) at 0.01 µg
Lane 1: Western blot - Recombinant Human SPG21/Maspardin protein (Recombinant Human SPG21/Maspardin protein ab86438)
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa, 35 kDa
Exposure time: 30s
SDS-PAGE - Recombinant Human TAGLN/Transgelin protein (ab101469)
SDS-PAGE analysis of ab101469 (3µg).
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