JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB198755

Recombinant human Tankyrase 2/TNKS2 protein (GST tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human Tankyrase 2/TNKS2 protein (GST tag N-Terminus) is a Human Fragment protein, in the 667 to 1166 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PARP5B, TANK2, TNKL, TNKS2, Poly [ADP-ribose] polymerase tankyrase-2, ADP-ribosyltransferase diphtheria toxin-like 6, Poly [ADP-ribose] polymerase 5B, Protein poly-ADP-ribosyltransferase tankyrase-2, TNKS-2, TRF1-interacting ankyrin-related ADP-ribose polymerase 2, Tankyrase II, Tankyrase-2, Tankyrase-like protein, Tankyrase-related protein, ARTD6

2 Images
Functional Studies - Recombinant human Tankyrase 2/TNKS2 protein (GST tag N-Terminus) (AB198755)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Tankyrase 2/TNKS2 protein (GST tag N-Terminus) (AB198755)

Activity assay using ab198755.

SDS-PAGE - Recombinant human Tankyrase 2/TNKS2 protein (GST tag N-Terminus) (AB198755)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Tankyrase 2/TNKS2 protein (GST tag N-Terminus) (AB198755)

4-20% SDS-PAGE analysis of 2.5 μg ab198755 with Coomassie staining.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific Activity: ≥16259 U/μg.

Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.

Assay Conditions: Enzyme reaction is conducted using a Tankyrase 2/TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+).

Accession

Q9H2K2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

style
left-column

Complementary Products

Proteins & Peptides

AB198639

Recombinant human Tankyrase protein (GST tag N-Terminus)

Functional Studies - Recombinant human Tankyrase protein (GST tag N-Terminus) (AB198639)

  • 0
  • 0
Proteins & Peptides

AB271546

Recombinant human TNFRSF14/HVEM protein (Active) (Fc tag C-Terminus)

Functional Studies - Recombinant human TNFRSF14/HVEM protein (Active) (AB271546)

  • 0
  • 0
Proteins & Peptides

AB79640

Recombinant human PARP6 protein (GST tag N-Terminus)

Functional Studies - Recombinant human PARP6 protein (GST tag N-Terminus) (AB79640)

  • 0
  • 0
Proteins & Peptides

AB271721

Recombinant human PRMT5 + MEP50 protein (Active)

Functional Studies - Recombinant human PRMT5 + MEP50 protein (Active) (AB271721)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Previously labelled as Tankyrase 2.
style
left-column

Sequence info

[{"sequence":"PDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQTPLDLVSADDVSALLTAAMPPSALPSCYKPQVLNGVRSPGATADALSSGPSSPSSLSAASSLDNLSGSFSELSSVVSSSGTEGASSLEKKEVPGVDFSITQFVRNLGLEHLMDIFEREQITLDVLVEMGHKELKEIGINAYGHRHKLIKGVERLISGQQGLNPYLTLNTSGSGTILIDLSPDDKEFQSVEEEMQSTVREHRDGGHAGGIFNRYNILKIQKVCNKKLWERYTHRRKEVSEENHNHANERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIMRPEGMVDG","proteinLength":"Fragment","predictedMolecularWeight":"80.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":1166,"aminoAcidStart":667,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9H2K2","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tankyrase 2 also known as TNKS2 functions as a poly(ADP-ribose) polymerase adding ADP-ribose polymers to target proteins. It has a molecular weight of approximately 135 kDa. Expression of TNKS2 occurs in various tissues including the brain heart and skeletal muscle. This protein consists of several domains such as an ankyrin repeat domain and a sterile alpha motif (SAM) domain allowing it to interact with different substrates and mediate protein-protein interactions.
Biological function summary

TNKS2 plays a significant role in telomere maintenance and Wnt signaling. It is a component of the telomere-associated complex which helps regulate telomere length and stability by interacting with TRF1 a telomere-binding factor. In the context of Wnt signaling it functions by modulating the stability of Axin a scaffold protein thereby affecting the degradation of β-catenin. By ensuring proper telomere function and Wnt signaling TNKS2 supports cellular processes such as proliferation and differentiation.

Pathways

TNKS2 contributes notably to telomere maintenance and Wnt/β-catenin signaling pathways. In telomere maintenance its activity influences telomerase access to telomeres cooperating with proteins like TRF1 and TIN2. Within the Wnt/β-catenin signaling pathway TNKS2 interacts with Axin promoting β-catenin accumulation which in turn stimulates gene transcription necessary for cell growth and development. These two pathways demonstrate how TNKS2 is involved in regulating fundamental aspects of cell biology and growth.

TNKS2 associates with conditions such as cancer and fibrosis. In various cancers aberrant TNKS2 activity results in dysregulation of Wnt signaling leading to improper cell proliferation and tumor growth. For example its interaction with β-catenin contributes to oncogenesis in colorectal cancer. In fibrosis altered TNKS2 function disrupts normal signaling processes which can lead to the excessive connective tissue accumulation characteristic of fibrotic diseases. Therefore TNKS2 appears as a promising target in therapeutic strategies aiming to modulate these pathways and control disease progression.
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified.

style
left-column

General info

Function

Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed : 11739745, PubMed : 11802774, PubMed : 19759537, PubMed : 21478859, PubMed : 23622245, PubMed : 25043379). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex : poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed : 19759537, PubMed : 21478859). Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed : 21478859). Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length (PubMed : 11739745). Stimulates 26S proteasome activity (PubMed : 23622245).

Sequence similarities

Belongs to the ARTD/PARP family.

Post-translational modifications

Ubiquitinated at 'Lys-48' and 'Lys-63' by RNF146 when auto-poly-ADP-ribosylated; this leads to degradation. Deubiquitinated by USP25; leading to stabilization (By similarity).. ADP-ribosylated (-auto). Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.. The crystallographic evidence suggests that the 3-hydroxyhistidine may be the (3S) stereoisomer.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed : 11739745, PubMed : 11802774, PubMed : 19759537, PubMed : 21478859, PubMed : 23622245, PubMed : 25043379). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex : poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed : 19759537, PubMed : 21478859). Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed : 21478859). Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length (PubMed : 11739745). Stimulates 26S proteasome activity (PubMed : 23622245).
See full target information TNKS2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com