Recombinant human Tankyrase 2/TNKS2 protein is a Human Fragment protein, in the 667 to 1166 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
P D N V N C R D T Q G R H S T P L H L A A G Y N N L E V A E Y L L Q H G A D V N A Q D K G G L I P L H N A A S Y G H V D V A A L L I K Y N A C V N A T D K W A F T P L H E A A Q K G R T Q L C A L L L A H G A D P T L K N Q E G Q T P L D L V S A D D V S A L L T A A M P P S A L P S C Y K P Q V L N G V R S P G A T A D A L S S G P S S P S S L S A A S S L D N L S G S F S E L S S V V S S S G T E G A S S L E K K E V P G V D F S I T Q F V R N L G L E H L M D I F E R E Q I T L D V L V E M G H K E L K E I G I N A Y G H R H K L I K G V E R L I S G Q Q G L N P Y L T L N T S G S G T I L I D L S P D D K E F Q S V E E E M Q S T V R E H R D G G H A G G I F N R Y N I L K I Q K V C N K K L W E R Y T H R R K E V S E E N H N H A N E R M L F H G S P F V N A I I H K G F D E R H A Y I G G M F G A G I Y F A E N S S K S N Q Y V Y G I G G G T G C P V H K D R S C Y I C H R Q L L F C R V T L G K S F L Q F S A M K M A H S P P G H H S V T G R P S V N G L A L A E Y V I Y R G E Q A Y P E Y L I T Y Q I M R P E G M V D G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed:11739745, PubMed:11802774, PubMed:19759537, PubMed:21478859, PubMed:23622245, PubMed:25043379). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed:19759537, PubMed:21478859). Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed:21478859). Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length (PubMed:11739745). Stimulates 26S proteasome activity (PubMed:23622245).
Alternative names
PARP5B, TANK2, TNKL, TNKS2, Poly [ADP-ribose] polymerase tankyrase-2, ADP-ribosyltransferase diphtheria toxin-like 6, Poly [ADP-ribose] polymerase 5B, Protein poly-ADP-ribosyltransferase tankyrase-2, TNKS-2, TRF1-interacting ankyrin-related ADP-ribose polymerase 2, Tankyrase II, Tankyrase-2, Tankyrase-like protein, Tankyrase-related protein, ARTD6
Recommended products
Recombinant human Tankyrase 2/TNKS2 protein is a Human Fragment protein, in the 667 to 1166 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: ≥16259 U/μg.
Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Assay Conditions: Enzyme reaction is conducted using a Tankyrase 2/TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+).
- Accession
- Q9H2K2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- P D N V N C R D T Q G R H S T P L H L A A G Y N N L E V A E Y L L Q H G A D V N A Q D K G G L I P L H N A A S Y G H V D V A A L L I K Y N A C V N A T D K W A F T P L H E A A Q K G R T Q L C A L L L A H G A D P T L K N Q E G Q T P L D L V S A D D V S A L L T A A M P P S A L P S C Y K P Q V L N G V R S P G A T A D A L S S G P S S P S S L S A A S S L D N L S G S F S E L S S V V S S S G T E G A S S L E K K E V P G V D F S I T Q F V R N L G L E H L M D I F E R E Q I T L D V L V E M G H K E L K E I G I N A Y G H R H K L I K G V E R L I S G Q Q G L N P Y L T L N T S G S G T I L I D L S P D D K E F Q S V E E E M Q S T V R E H R D G G H A G G I F N R Y N I L K I Q K V C N K K L W E R Y T H R R K E V S E E N H N H A N E R M L F H G S P F V N A I I H K G F D E R H A Y I G G M F G A G I Y F A E N S S K S N Q Y V Y G I G G G T G C P V H K D R S C Y I C H R Q L L F C R V T L G K S F L Q F S A M K M A H S P P G H H S V T G R P S V N G L A L A E Y V I Y R G E Q A Y P E Y L I T Y Q I M R P E G M V D G
- Accession
- Q9H2K2
- Protein length
- Fragment
- Predicted molecular weight
- 80.5 kDa
- Amino acids
- 667 to 1166
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed:11739745, PubMed:11802774, PubMed:19759537, PubMed:21478859, PubMed:23622245, PubMed:25043379). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed:19759537, PubMed:21478859). Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed:21478859). Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length (PubMed:11739745). Stimulates 26S proteasome activity (PubMed:23622245).
- Sequence similarities
Belongs to the ARTD/PARP family.
- Post-translational modifications
Ubiquitinated at 'Lys-48' and 'Lys-63' by RNF146 when auto-poly-ADP-ribosylated; this leads to degradation. Deubiquitinated by USP25; leading to stabilization (By similarity).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Previously labelled as Tankyrase 2.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tankyrase 2 also known as TNKS2 functions as a poly(ADP-ribose) polymerase adding ADP-ribose polymers to target proteins. It has a molecular weight of approximately 135 kDa. Expression of TNKS2 occurs in various tissues including the brain heart and skeletal muscle. This protein consists of several domains such as an ankyrin repeat domain and a sterile alpha motif (SAM) domain allowing it to interact with different substrates and mediate protein-protein interactions.
- Biological function summary
TNKS2 plays a significant role in telomere maintenance and Wnt signaling. It is a component of the telomere-associated complex which helps regulate telomere length and stability by interacting with TRF1 a telomere-binding factor. In the context of Wnt signaling it functions by modulating the stability of Axin a scaffold protein thereby affecting the degradation of β-catenin. By ensuring proper telomere function and Wnt signaling TNKS2 supports cellular processes such as proliferation and differentiation.
- Pathways
TNKS2 contributes notably to telomere maintenance and Wnt/β-catenin signaling pathways. In telomere maintenance its activity influences telomerase access to telomeres cooperating with proteins like TRF1 and TIN2. Within the Wnt/β-catenin signaling pathway TNKS2 interacts with Axin promoting β-catenin accumulation which in turn stimulates gene transcription necessary for cell growth and development. These two pathways demonstrate how TNKS2 is involved in regulating fundamental aspects of cell biology and growth.
- Associated diseases and disorders
TNKS2 associates with conditions such as cancer and fibrosis. In various cancers aberrant TNKS2 activity results in dysregulation of Wnt signaling leading to improper cell proliferation and tumor growth. For example its interaction with β-catenin contributes to oncogenesis in colorectal cancer. In fibrosis altered TNKS2 function disrupts normal signaling processes which can lead to the excessive connective tissue accumulation characteristic of fibrotic diseases. Therefore TNKS2 appears as a promising target in therapeutic strategies aiming to modulate these pathways and control disease progression.
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2 product images
Functional Studies - Recombinant human Tankyrase 2/TNKS2 protein (ab198755)
Activity assay using ab198755.
SDS-PAGE - Recombinant human Tankyrase 2/TNKS2 protein (ab198755)
4-20% SDS-PAGE analysis of 2.5 μg ab198755 with Coomassie staining.
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