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AB198639

Recombinant human Tankyrase protein (GST tag N-Terminus)

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Recombinant human Tankyrase protein (GST tag N-Terminus) is a Human Fragment protein, in the 1001 to 1327 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PARP5A, PARPL, TIN1, TINF1, TNKS1, TNKS, Poly [ADP-ribose] polymerase tankyrase-1, ADP-ribosyltransferase diphtheria toxin-like 5, Poly [ADP-ribose] polymerase 5A, Protein poly-ADP-ribosyltransferase tankyrase-1, TNKS-1, TRF1-interacting ankyrin-related ADP-ribose polymerase, Tankyrase I, Tankyrase-1, ARTD5, TANK1

2 Images
Functional Studies - Recombinant human Tankyrase protein (GST tag N-Terminus) (AB198639)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Tankyrase protein (GST tag N-Terminus) (AB198639)

Specific activity of ab198639.

SDS-PAGE - Recombinant human Tankyrase protein (GST tag N-Terminus) (AB198639)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Tankyrase protein (GST tag N-Terminus) (AB198639)

4-20% SDS-PAGE with Coomassie staining

Lane 1 : 3.8 μg ab198639
Lane 2 : Protein marker

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.

Accession

O95271

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.48% Tris buffer, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.017% Potassium chloride

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"ELAVGGASNAGDGAAGTERKEGEVAGLDMNISQFLKSLGLEHLRDIFETEQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERLLGGQQGTNPYLTFHCVNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRIQKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPEAPSQTATAAEQKT","proteinLength":"Fragment","predictedMolecularWeight":"62.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":1327,"aminoAcidStart":1001,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O95271","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tankyrase also known as TNKS and tankyrase-1 is an enzyme belonging to the poly(ADP-ribose) polymerase (PARP) family with a molecular mass of about 142 kDa. It is expressed in various tissues including brain heart and liver as well as in certain types of cancer cells. Mechanically tankyrase catalyzes the addition of ADP-ribose polymers to protein substrates a process known as poly(ADP-ribosyl)ation. This activity modulates the stability localization or interactions of target proteins.
Biological function summary

Tankyrase functions in diverse cellular processes. It participates in telomere maintenance by ADP-ribosylating TRF1 a telomere-binding protein leading to TRF1's disassembly from telomeres. Moreover tankyrase acts in the regulation of Wnt/β-catenin signaling where it associates with components like AXIN influencing the stability of the β-catenin destruction complex. Tankyrase can also be part of larger protein complexes such as the shelterin complex playing a role in safeguarding chromosome ends.

Pathways

Tankyrase integrates into key signaling networks. In the Wnt/β-catenin pathway tankyrase's interaction with AXIN controls the availability of β-catenin an important transcription co-activator in cell proliferation and differentiation. This enzyme also connects with the telomere maintenance pathway engaging TRF1 and contributing to telomere elongation and stability. Through these pathways tankyrase engages in processes vital for cell growth and genome integrity.

Tankyrase is implicated in cancer and heritable disorders of telomere dysfunction. Abnormal tankyrase activity correlates with increased β-catenin levels promoting oncogenesis in colorectal and other cancers. Additionally disruptions in tankyrase-mediated telomere regulation can lead to conditions like dyskeratosis congenita. Tankyrase links with proteins such as β-catenin in cancer while in telomere-related disorders it connects with TRF1 indicating its significance in maintaining cellular homeostasis.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed : 10988299, PubMed : 11739745, PubMed : 16076287, PubMed : 19759537, PubMed : 21478859, PubMed : 22864114, PubMed : 23622245, PubMed : 25043379, PubMed : 28619731). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex : poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed : 19759537, PubMed : 21478859). Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed : 21478859). Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length (PubMed : 11739745). Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI (PubMed : 22864114). May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles (PubMed : 10988299). May be involved in spindle pole assembly through PARsylation of NUMA1 (PubMed : 16076287). Stimulates 26S proteasome activity (PubMed : 23622245).

Sequence similarities

Belongs to the ARTD/PARP family.

Post-translational modifications

Phosphorylated on serine residues by MAPK kinases upon insulin stimulation. Phosphorylated during mitosis.. Ubiquitinated by RNF146 when auto-poly-ADP-ribosylated, leading to its degradation. Deubiquitinated by USP25; leading to stabilization (PubMed:28619731).. ADP-ribosylated (-auto). Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed : 10988299, PubMed : 11739745, PubMed : 16076287, PubMed : 19759537, PubMed : 21478859, PubMed : 22864114, PubMed : 23622245, PubMed : 25043379, PubMed : 28619731). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex : poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed : 19759537, PubMed : 21478859). Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed : 21478859). Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length (PubMed : 11739745). Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI (PubMed : 22864114). May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles (PubMed : 10988299). May be involved in spindle pole assembly through PARsylation of NUMA1 (PubMed : 16076287). Stimulates 26S proteasome activity (PubMed : 23622245).
See full target information TNKS
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