JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB159637

Recombinant Human TAP1 protein (GST tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human TAP1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 808 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

ABCB2, PSF1, RING4, Y3, TAP1, Antigen peptide transporter 1, APT1, ATP-binding cassette sub-family B member 2, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter TAP1, Peptide transporter involved in antigen processing 1, Really interesting new gene 4 protein, PSF-1

1 Images
SDS-PAGE - Recombinant Human TAP1 protein (GST tag N-Terminus) (AB159637)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human TAP1 protein (GST tag N-Terminus) (AB159637)

ab159637 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q03518

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

style
left-column

Complementary Products

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

  • 0
  • 0
Proteins & Peptides

AB104637

Recombinant Human NELFe protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human NELFe protein (His tag N-Terminus) (AB104637)

  • 0
  • 0
Primary Antibodies

AB307282

Anti-TAP2 antibody [EPR26237-82]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry - Anti-TAP2 antibody [EPR26237-82] (AB307282)

  • 0
  • 0
Primary Antibodies

AB137013

Anti-SEC14L2/TAP antibody [EPR8612]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC14L2/TAP antibody [EPR8612] (AB137013)

  • 0
  • 0
Primary Antibodies

AB31645

Anti-hnRNP A2B1 antibody

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A2B1 antibody (AB31645)

  • 5
  • 5
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MAELLASAGSACSWDFPRAPPSFPPPAASRGGLGGTRSFRPHRGAESPRPGRDRDGVRVPMASSRCPAPRGCRCLPGASLAWLGTVLLLLADWVLLRTALPRIFSLLVPTALPLLRVWAVGLSRWAVLWLGACGVLRATVGSKSENAGAQGWLAALKPLAAALGLALPGLALFRELISWGAPGSADSTRLLHWGSHPTAFVVSYAAALPAAALWHKLGSLWVPGGQGGSGNPVRRLLGCLGSETRRLSLFLVLVVLSSLGEMAIPFFTGRLTDWILQDGSADTFTRNLTLMSILTIASAVLEFVGDGIYNNTMGHVHSHLQGEVFGAVLRQETEFFQQNQTGNIMSRVTEDTSTLSDSLSENLSLFLWYLVRGLCLLGIMLWGSVSLTMVTLITLPLLFLLPKKVGKWYQLLEVQVRESLAKSSQVAIEALSAMPTVRSFANEEGEAQKFREKLQEIKTLNQKEAVAYAVNSWTTSISGMLLKVGILYIGGQLVTSGAVSSGNLVTFVLYQMQFTQAVEVLLSIYPRVQKAVGSSEKIFEYLDRTPRCPPSGLLTPLHLEGLVQFQDVSFAYPNRPDVLVLQGLTFTLRPGEVTALVGPNGSGKSTVAALLQNLYQPTGGQLLLDGKPLPQYEHRYLHRQVAAVGQEPQVFGRSLQENIAYGLTQKPTMEEITAAAVKSGAHSFISGLPQGYDTEVDEAGSQLSGGQRQAVALARALIRKPCVLILDDATSALDANSQLQVEQLLYESPERYSRSVLLITQHLSLVEQADHILFLEGGAIREGGTHQQLMEKKGCYWAMVQAPADAPE","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":808,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q03518","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The transporter associated with antigen processing 1 (TAP1) also known as ABCB2 is an ATP-binding cassette transporter with an important role in cellular immunity. TAP1 functions mechanically by transporting peptides from the cytosol into the endoplasmic reticulum. These peptides are typically 8-16 amino acids long and are processed for MHC class I molecules presentation. TAP1 has a molecular mass of approximately 75 kDa. Expression of TAP1 occurs primarily in cells of the immune system including lymphocytes and dendritic cells.
Biological function summary

TAP1 participates in the immune system by facilitating the presentation of peptide antigens to T cells. It forms a complex with TAP2 to transport antigenic peptides necessary for the proper functioning of the MHC class I pathway. This complex is essential for the immune system's ability to detect and respond to pathogens. Proper functioning of TAP1 ensures that peptides presented on MHC class I molecules are of endogenous origin allowing T cells to monitor cellular health and detect infected or transformed cells.

Pathways

TAP1 plays a role in the antigen processing and presentation pathway which is critical for adaptive immunity. This pathway involves specific steps in peptide processing where TAP1 along with TAP2 is essential for translocating peptides for binding to MHC class I complexes. Other related proteins in this pathway include calreticulin and tapasin which assist in peptide loading onto MHC class I molecules. Additionally TAP1 is involved in regulating cytosolic proteins' turnover through its role in transporting peptides that result from degraded proteins.

TAP1 dysfunction or variants can lead to immune system-related diseases such as Bare Lymphocyte Syndrome (BLS) a condition characterized by the lack of MHC class I molecule expression. This results in compromised immune surveillance and increased susceptibility to infections. TAP1 is also linked to autoimmune diseases where abnormal peptide presentation may occur. It is associated with proteins like HLA molecules which are often implicated in autoimmune pathogenesis. Analyzing TAP1 expression and function provides insight into these disorders' underlying mechanisms.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

ABC transporter associated with antigen processing. In complex with TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules (PubMed : 25377891, PubMed : 25656091). Uses the chemical energy of ATP to export peptides against the concentration gradient (PubMed : 25377891). During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle (PubMed : 11274390, PubMed : 25377891, PubMed : 25656091). Typically transports intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome. Binds peptides with free N- and C-termini, the first three and the C-terminal residues being critical. Preferentially selects peptides having a highly hydrophobic residue at position 3 and hydrophobic or charged residues at the C-terminal anchor. Proline at position 2 has the most destabilizing effect (PubMed : 11274390, PubMed : 7500034, PubMed : 9256420). As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation (PubMed : 1538751, PubMed : 25377891, PubMed : 26611325).

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.

style
left-column

Product protocols

style
left-column

Target data

ABC transporter associated with antigen processing. In complex with TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules (PubMed : 25377891, PubMed : 25656091). Uses the chemical energy of ATP to export peptides against the concentration gradient (PubMed : 25377891). During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle (PubMed : 11274390, PubMed : 25377891, PubMed : 25656091). Typically transports intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome. Binds peptides with free N- and C-termini, the first three and the C-terminal residues being critical. Preferentially selects peptides having a highly hydrophobic residue at position 3 and hydrophobic or charged residues at the C-terminal anchor. Proline at position 2 has the most destabilizing effect (PubMed : 11274390, PubMed : 7500034, PubMed : 9256420). As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation (PubMed : 1538751, PubMed : 25377891, PubMed : 26611325).
See full target information TAP1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com