Recombinant human Tartrate Resistant Acid Phosphatase protein (Active) is a Human Full Length protein, in the 22 to 325 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
A T P A L R F V A V G D W G G V P N A P F H T A R E M A N A K E I A R T V Q I L G A D F I L S L G D N F Y F T G V Q D I N D K R F Q E T F E D V F S D R S L R K V P W Y V L A G N H D H L G N V S A Q I A Y S K I S K R W N F P S P F Y R L H F K I P Q T N V S V A I F M L D T V T L C G N S D D F L S Q Q P E R P R D V K L A R T Q L S W L K K Q L A A A R E D Y V L V A G H Y P V W S I A E H G P T H C L V K Q L R P L L A T Y G V T A Y L C G H D H N L Q Y L Q D E N G V G Y V L S G A G N F M D P S K R H Q R K V P N G Y L R F H Y G T E D S L G G F A Y V E I S S K E M T V T Y I E A S G K S L F K T R L P R R A R P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
Alternative names
Tartrate-resistant acid phosphatase type 5, TR-AP, Tartrate-resistant acid ATPase, Type 5 acid phosphatase, TrATPase, ACP5
Recommended products
Recombinant human Tartrate Resistant Acid Phosphatase protein (Active) is a Human Full Length protein, in the 22 to 325 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity is >10,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37°C.
- Accession
- P13686-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A T P A L R F V A V G D W G G V P N A P F H T A R E M A N A K E I A R T V Q I L G A D F I L S L G D N F Y F T G V Q D I N D K R F Q E T F E D V F S D R S L R K V P W Y V L A G N H D H L G N V S A Q I A Y S K I S K R W N F P S P F Y R L H F K I P Q T N V S V A I F M L D T V T L C G N S D D F L S Q Q P E R P R D V K L A R T Q L S W L K K Q L A A A R E D Y V L V A G H Y P V W S I A E H G P T H C L V K Q L R P L L A T Y G V T A Y L C G H D H N L Q Y L Q D E N G V G Y V L S G A G N F M D P S K R H Q R K V P N G Y L R F H Y G T E D S L G G F A Y V E I S S K E M T V T Y I E A S G K S L F K T R L P R R A R P
- Accession
- P13686
- Protein length
- Full Length
- Predicted molecular weight
- 34.3 kDa
- Amino acids
- 22 to 325
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab222972 was purified by using conventional chromatography techniques.
General info
- Function
Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
- Sequence similarities
Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
- Subcellular localisation
- Lysosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tartrate Resistant Acid Phosphatase (TRAP) also known as ACP-5 and type 5 acid phosphatase is an enzyme with mass approximately 35 kDa. It is expressed in osteoclasts macrophages and dendritic cells playing a role in bone resorption and macrophage activation. TRAP exists in both a monomeric and proteolytically activated dimeric form. Its unique tartrate resistance distinguishes it from other acid phosphatases providing specificity for enzyme assays and studies.
- Biological function summary
TRAP contributes significantly to bone remodeling through its action in osteoclasts. It helps in degrading mineralized matrix components and may also serve a role in dendritic cell activation signaling and macrophage-related processes. TRAP forms part of a complex mechanism that includes catabolic enzymes which work together to ensure normal skeletal function and immune response.
- Pathways
TRAP plays an integral role in the bone resorption pathway along with proteins such as cathepsin K and matrix metalloproteinases. It is involved in osteoclast differentiation necessary for bone turnover and homeostasis. Additionally it participates in the immune response through its activity in macrophages affecting inflammation pathways and innate immune functions.
- Associated diseases and disorders
Abnormal TRAP activity associates with osteopetrosis and osteoporosis. These diseases affect bone density and strength with TRAP often showing elevated levels in conditions of high bone turnover. TRAP activity also connects to inflammatory disorders due to its expression in macrophages linking it to proteins like RANKL and osteoprotegerin which influence bone metabolism and immune signaling.
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1 product image
SDS-PAGE - Recombinant human Tartrate Resistant Acid Phosphatase protein (Active) (ab222972)
15% SDS-PAGE - Human recombinant Polyclonal to Tartrate Resistant Acid Phosphatase protein (3 μg ab222972).
28-40 KDa under reducing conditions.
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